LOCUS       sequence-19226-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf149
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             612..653
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             663..680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(703..720)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggtaccgagc tcggatccct agtccagtgt ggtggaattg tcttgaccct tcaccgaatt
       61 ccagctgacc accatgttca acatgaatat taactcacca gttagatttg ttaaggaaac
      121 taacagagct aaatctccta ctaggcaatc accttacgcc gccggatatg atttatatag
      181 cgcttacgat tatactatcc ctccaggaga acgacagtta attaagacag atattagtat
      241 gtccatgcct aagttctgct atggtagaat agctcctagg tctggtctgt ccctaaaagg
      301 cattgatata ggaggcggtg taatagacga agattatagg ggaaacatgg gagtcattct
      361 tattaataat ggaaaatgta cgtttaatgt aaatactgga gatagaatag ctcagctaat
      421 ctatcaacgt atatattatc cagaactgga agaagtacaa tctctagata gtacaaatag
      481 aggagatcaa gggtttggat caacaggact tagataagca attcccgggg atccgaataa
      541 agggtcaaga caattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
      601 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
      661 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
      721 gttg
//
