LOCUS       sequence-19232-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf155
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             296..337
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             347..364
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(387..404)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    393..618
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accatggagg gatctaaacg caaacacgac agtcggcgac tacaacaaga acaggagcag
       61 cttcgtccac gtacaccgcc atcatatgaa gaaattgcaa aatatggaca ctcatttaac
      121 gtgaaaagat ttacgaatga agaaatgtgt cttaagaatg attatccacg aattatatca
      181 tataatcctc caccaaaata ggcaattccc ggggatccga ataaagggtc aagacaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
      301 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
      361 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      421 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      481 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
      541 tgggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
      601 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
      661 cccacgcgcc ctgtagcggc gcatta
//
