LOCUS       sequence-192347-       11322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae 
            bacterium ND2006 (LbCpf1), codon optimized for expression in 
            Saccharomyces cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA
  TITLE     CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
  PUBMED    28886218
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2017
            Sep 8. doi: 10.1002/yea.3278."
COMMENT     Sequence Label: pCSN067
FEATURES             Location/Qualifiers
     source          1..11322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2900..6583
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             6596..6616
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    6965..6998
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            7044..8400
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        7044..7387
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7388..8197
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7455..7474)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      8203..8400
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    8448..8481
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8548..8568)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8548..8566)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8587..8603)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8587..8603)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8600..8622)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8611..8627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8635..8641,8642..8659,8660..8665))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8818..8835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8989..9577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9069..9088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9748..10539,10540..10608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10371..10390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10609..10713)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10750..11253
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     11295..11313
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtacct tttctttttt tgcggtcacc
     1921 cccatgtggc ggggaggcag aggagtaggt agagcaacga atcctactat ttatccaaat
     1981 tagtctagga actctttttc tagatttttt agatttgagg gcaagcgctg ttaacgactc
     2041 agaaatgtaa gcactacgga gtagaacgag aaatccgcca taggtggaaa tcctagcaaa
     2101 atcttgctta ccctagctag cctcaggtaa gctagcctta gcctgtcaaa tttttttcaa
     2161 aatttggtaa gtttctacta gcaaagcaaa cacggttcaa caaaccgaaa actccactca
     2221 ttatacgtgg aaaccgaaac aaaaaaacaa aaaccaaaat actcgccaat gagaaagttg
     2281 ctgcgtttct actttcgagg aagaggaact gagaggattg actacgaaag gggcaaaaac
     2341 gagtcgtatt ctcccattat tgtctgctac cacgcggtct agtagaataa gcaaccagtc
     2401 aacgctaaga caggtaatca aaataccagt ctgctggcta cgggctagtt tttacctctt
     2461 ttagaaccca ctgtaaaagt ccgttgtaaa gcccgttctc actgttggcg tttttttttt
     2521 tttggtttag tttcttattt ttcatttttt tctttcatga ccaaaaacaa acaaatctcg
     2581 cgatttgtac tgcggccact ggggcgtggc caaaaaaatg acaaatttag aaaccttagt
     2641 ttctgatttt tcctgttatg aggagatatg ataaaaaata ttactgcttt attgtttttt
     2701 ttttatctac tgaaatagag aaacttaccc aaggaggagg caaaaaaaag agtatatata
     2761 cagcagctac cattcagatt ttaatatatt cttttctctt cttctacact attattataa
     2821 taattttact atattcattt ttagcttaaa acctcataga atattattct tcagtcactc
     2881 gcttaaatac ttatcaaaaa tgtctaagtt ggaaaaattc accaactgtt actctttgtc
     2941 taagactttg agattcaagg ccatcccagt tggtaagacc caagaaaaca tcgacaacaa
     3001 gagactatta gttgaagatg aaaagagagc tgaagactac aagggtgtca agaaattgtt
     3061 ggacagatac tacttgtctt ttatcaacga cgttttgcat tccatcaagc taaagaactt
     3121 gaataactac atctctttgt tcagaaagaa gactagaact gaaaaggaaa ataaggaatt
     3181 ggaaaacttg gaaatcaact tgagaaagga aattgctaag gctttcaagg gtaatgaagg
     3241 ttacaagtct ttattcaaga aagacatcat tgaaaccatt ttgccagaat ttttggatga
     3301 taaggatgaa attgctttgg ttaactcttt caacggtttc accactgctt tcactggttt
     3361 cttcgacaac agagaaaaca tgttctccga ggaagctaaa tccacttcta ttgctttcag
     3421 atgtatcaac gaaaacttga cccgttacat ctctaacatg gacatttttg aaaaggtcga
     3481 cgccatcttt gacaagcacg aagtccaaga aatcaaggaa aagatcttaa actccgacta
     3541 cgatgtcgaa gatttcttcg aaggtgaatt cttcaacttt gttttaaccc aagaaggtat
     3601 cgatgtctac aacgccatta tcggtggttt tgtcactgaa tctggtgaaa agatcaaggg
     3661 tttgaacgaa tacattaact tgtacaacca aaagaccaaa caaaaattgc caaagttcaa
     3721 gccattgtac aagcaagttt tgtctgacag agaatctttg tctttttacg gtgaagggta
     3781 cacctctgac gaagaagtct tggaagtctt cagaaacact ttgaacaaga actctgaaat
     3841 cttctcctcc atcaagaagt tagaaaagtt gttcaagaac ttcgatgaat actcttctgc
     3901 tggtatcttc gttaagaacg gtccagccat ctctaccatt tctaaggata tctttggtga
     3961 atggaacgtc attagagaca aatggaacgc tgaatacgat gacatccatt tgaagaaaaa
     4021 ggctgttgtc accgaaaagt acgaagacga cagaagaaaa tccttcaaga agatcggttc
     4081 cttctccttg gaacaattac aagaatacgc cgatgccgat ttgtccgttg tcgaaaaatt
     4141 gaaggaaatt attattcaaa aggttgatga aatttacaaa gtttacggtt cctctgaaaa
     4201 gttattcgat gctgatttcg tcttggaaaa gtctttgaag aagaacgacg ctgttgtcgc
     4261 tatcatgaag gacttgttgg actctgtcaa atctttcgaa aactatatca aggccttctt
     4321 cggtgaaggt aaggaaacta acagagatga atccttctac ggtgactttg tcttggctta
     4381 cgatattttg ttgaaggttg accacatcta cgatgccatc agaaactacg ttactcaaaa
     4441 gccatactct aaggacaaat tcaagttgta cttccaaaac ccacaattca tgggtggttg
     4501 ggataaggac aaggaaactg actacagagc taccattttg agatacggtt ccaagtacta
     4561 cttggccatc atggacaaga agtacgccaa gtgtttgcaa aagattgaca aggacgatgt
     4621 caacggtaac tacgaaaaga ttaactacaa gttgttgcca ggtccaaaca agatgttgcc
     4681 aaaggttttc ttctccaaaa agtggatggc ttactacaac ccatctgaag acatccaaaa
     4741 gatctacaag aacggtactt tcaaaaaggg tgacatgttc aacttaaacg actgtcacaa
     4801 gttgatcgac ttcttcaagg actccatctc tagataccca aaatggtcca acgcttacga
     4861 tttcaacttc tctgaaactg aaaaatacaa ggatattgct ggtttctacc gtgaagtcga
     4921 ggaacaaggt tataaggttt ctttcgaatc cgcttctaag aaagaagttg acaaattagt
     4981 cgaagaaggt aagttgtaca tgttccaaat ctacaacaaa gatttctccg acaagtctca
     5041 cggtactcca aacttgcaca ccatgtactt caagttgcta ttcgatgaaa acaaccacgg
     5101 tcaaatcaga ttgtctggtg gtgctgaatt gttcatgaga cgtgcttctc taaagaagga
     5161 agaattagtc gtccacccag ctaactctcc aattgccaac aagaacccag acaaccctaa
     5221 gaagaccacc actttgtcct acgacgttta caaggacaag agattctccg aagaccaata
     5281 cgaattgcac attccaattg ctatcaacaa gtgtccaaag aacatcttca agatcaacac
     5341 tgaagtcaga gttttgttaa agcacgatga caacccttac gttattggta tcgaccgtgg
     5401 tgaaagaaat ttgttgtaca ttgttgttgt tgacggtaag ggtaacatcg ttgaacaata
     5461 ctccttgaac gaaatcatca acaacttcaa cggtattaga atcaagactg attaccactc
     5521 tttgttggat aagaaggaaa aggaacgttt tgaagctcgt caaaactgga cctctattga
     5581 aaacatcaaa gaattgaagg ctggttacat cagtcaagtt gtccacaaga tctgtgaatt
     5641 ggtcgagaag tacgatgccg ttattgcctt ggaagatttg aactctggtt ttaagaactc
     5701 tcgtgtcaag gttgaaaagc aagtctacca aaagttcgaa aagatgttaa tcgacaaatt
     5761 gaactacatg gttgacaaga aatccaaccc atgtgctacc ggtggtgctt tgaaaggtta
     5821 ccaaatcacc aacaaattcg aatctttcaa atctatgtcc actcaaaacg ggttcatctt
     5881 ctacattcca gcttggttga cctccaagat cgacccatct accggtttcg ttaacttgtt
     5941 gaagaccaag tacacttcca ttgctgattc caagaagttc atctcttctt tcgacagaat
     6001 catgtacgtt ccagaagaag acttgttcga attcgccttg gactataaga acttctccag
     6061 aaccgatgct gactacatta agaaatggaa attgtactcc tacggtaaca gaatcagaat
     6121 tttcagaaac ccaaagaaaa acaacgtttt cgattgggaa gaagtttgtt tgacttctgc
     6181 ctacaaggaa ttattcaaca aatacggtat caactaccaa caaggtgata tcagagcttt
     6241 gttgtgtgaa caatctgaca aggctttcta ctcttccttc atggctttga tgtccttgat
     6301 gttgcaaatg agaaactcca tcactggtag aactgatgtc gacttcctca tttctccagt
     6361 taagaattct gacggtattt tctacgactc tagaaattac gaagctcaag aaaacgctat
     6421 tttgccaaag aacgctgatg ctaacggtgc ttacaatatt gctagaaagg ttttgtgggc
     6481 tatcggtcaa ttcaagaagg ctgaagacga aaagctagac aaggtcaaga ttgctatttc
     6541 taacaaggaa tggttggaat acgctcaaac ctccgtcaag cactccagag ctgatccaaa
     6601 gaagaagaga aaggtataaa ggagttaaag gcaaagtttt cttttctaga gccgttccca
     6661 caaataatta tacgtatatg cttcttttcg tttactatat atctatattt acaagccttt
     6721 attcactgat gcaatttgtt tccaaatact tttttggaga tctcataact agatatcatg
     6781 atggcgcaac ttggcgctat cttaattact ctggctgcca ggcccgtgta gagggccgca
     6841 agaccttctg tacgccatat agtctctaag aacttgaaca agtttctaga cctattgccg
     6901 cctttcggat cgctattgtt gcggccgcca gctgaagctt cgtacgctgc aggtcgacga
     6961 attctaccgt tcgtataatg tatgctatac gaagttatag atctgtttag cttgcctcgt
     7021 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
     7081 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
     7141 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
     7201 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
     7261 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
     7321 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
     7381 aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga
     7441 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     7501 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     7561 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     7621 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     7681 gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     7741 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     7801 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     7861 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     7921 agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     7981 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     8041 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     8101 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     8161 attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt
     8221 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa
     8281 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag
     8341 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg
     8401 ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctcata acttcgtata
     8461 atgtatgcta tacgaacggt agaattcgaa tcagatccac tagtggccta tgcggccgcc
     8521 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     8581 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8641 aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt
     8701 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8761 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9421 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9721 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9781 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9841 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9901 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9961 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10021 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10081 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10141 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10201 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10261 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10321 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10381 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10441 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10501 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10561 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10621 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10681 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
    10741 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
    10801 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
    10861 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
    10921 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
    10981 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
    11041 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
    11101 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
    11161 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
    11221 tacgaggcgc gtgtaagtta caggcaagcg atccgtccta agaaaccatt attatcatga
    11281 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
