LOCUS       sequence-192349-       11538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing Cpf1 from Francisella novicida 
            U112 (FnCpf1), codon optimized for expression in Saccharomyces 
            cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA
  TITLE     CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
  PUBMED    28886218
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2017
            Sep 8. doi: 10.1002/yea.3278."
COMMENT     Sequence Label: pCSN068
FEATURES             Location/Qualifiers
     source          1..11538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6812..6832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    7181..7214
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            7260..8616
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        7260..7603
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7604..8413
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7671..7690)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      8419..8616
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    8664..8697
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8764..8784)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8764..8782)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8803..8819)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8803..8819)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8816..8838)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8827..8843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8851..8857,8858..8875,8876..8881))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(9034..9051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9205..9793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9285..9304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9964..10755,10756..10824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10587..10606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10825..10929)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10966..11469
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     11511..11529
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtacct tttctttttt tgcggtcacc
     1921 cccatgtggc ggggaggcag aggagtaggt agagcaacga atcctactat ttatccaaat
     1981 tagtctagga actctttttc tagatttttt agatttgagg gcaagcgctg ttaacgactc
     2041 agaaatgtaa gcactacgga gtagaacgag aaatccgcca taggtggaaa tcctagcaaa
     2101 atcttgctta ccctagctag cctcaggtaa gctagcctta gcctgtcaaa tttttttcaa
     2161 aatttggtaa gtttctacta gcaaagcaaa cacggttcaa caaaccgaaa actccactca
     2221 ttatacgtgg aaaccgaaac aaaaaaacaa aaaccaaaat actcgccaat gagaaagttg
     2281 ctgcgtttct actttcgagg aagaggaact gagaggattg actacgaaag gggcaaaaac
     2341 gagtcgtatt ctcccattat tgtctgctac cacgcggtct agtagaataa gcaaccagtc
     2401 aacgctaaga caggtaatca aaataccagt ctgctggcta cgggctagtt tttacctctt
     2461 ttagaaccca ctgtaaaagt ccgttgtaaa gcccgttctc actgttggcg tttttttttt
     2521 tttggtttag tttcttattt ttcatttttt tctttcatga ccaaaaacaa acaaatctcg
     2581 cgatttgtac tgcggccact ggggcgtggc caaaaaaatg acaaatttag aaaccttagt
     2641 ttctgatttt tcctgttatg aggagatatg ataaaaaata ttactgcttt attgtttttt
     2701 ttttatctac tgaaatagag aaacttaccc aaggaggagg caaaaaaaag agtatatata
     2761 cagcagctac cattcagatt ttaatatatt cttttctctt cttctacact attattataa
     2821 taattttact atattcattt ttagcttaaa acctcataga atattattct tcagtcactc
     2881 gcttaaatac ttatcaaaaa tgtccatcta ccaagagttt gtcaacaaat actctttgtc
     2941 taagacttta cgtttcgaat tgattccaca aggtaagact ttggaaaaca ttaaggctcg
     3001 tggtttgatc ttggacgacg aaaagagagc caaggattac aagaaggcca agcaaatcat
     3061 cgataagtac caccaattct tcattgaaga aatcttatcc tctgtctgta tctccgaaga
     3121 tctattgcaa aactactccg atgtctactt caagttgaaa aagtctgacg atgacaactt
     3181 gcaaaaggat ttcaaatctg ccaaggacac catcaagaaa caaatttctg aatacataaa
     3241 ggactctgaa aaatttaaga acttattcaa ccaaaacttg atcgatgcta agaagggtca
     3301 agaatctgac ttgatcttgt ggttgaagca atctaaggac aacggtatcg aattgttcaa
     3361 ggctaactct gatatcactg acattgacga agctttggaa atcatcaagt ctttcaaagg
     3421 ttggactacc tatttcaagg gtttccacga aaaccgtaag aatgtctact cttccaacga
     3481 catcccaact tccatcattt acagaatcgt tgacgacaat ttgccaaagt tcctagaaaa
     3541 caaggccaaa tacgaatcct tgaaggacaa ggctccagaa gccattaact acgaacaaat
     3601 caagaaggac ttggctgaag aattaacttt cgacattgac tacaagactt ctgaagttaa
     3661 ccaaagagtt ttctctttgg acgaagtctt cgagattgct aacttcaaca actacttgaa
     3721 ccaatctggt atcaccaaat tcaacaccat catcggtggt aagttcgtca acggtgaaaa
     3781 caccaagaga aagggtatca acgaatacat taacttgtac tctcagcaaa tcaacgacaa
     3841 gactttaaag aaatacaaga tgtctgtttt gttcaagcaa attttgtctg acactgaatc
     3901 caagtctttt gtcattgata agttggaaga tgattccgac gtcgttacca ccatgcaatc
     3961 tttctacgag caaatcgctg ctttcaagac cgttgaagaa aagtctatta aggaaacttt
     4021 gtctttgttg ttcgacgatt tgaaggctca aaagttggat ttgtccaaga tttacttcaa
     4081 gaatgacaag tctttgactg atttgtctca acaagttttc gatgactact ccgttattgg
     4141 tactgctgtc ttggaataca tcacccaaca aatcgctcct aagaacttgg acaacccatc
     4201 caagaaggaa caagaattga ttgccaagaa gaccgaaaaa gctaaatact tgtctttgga
     4261 aaccattaaa ttggctttag aagagttcaa caagcacaga gatattgaca agcaatgtag
     4321 attcgaagaa attttggcta acttcgctgc tatcccaatg atcttcgacg aaattgctca
     4381 aaacaaggat aacttggctc aaatctccat caagtaccaa aaccagggta agaaggattt
     4441 gttgcaagcc tccgctgaag atgacgtcaa ggccatcaaa gatttattgg accaaactaa
     4501 caacttgttg cacaagctaa agatcttcca catctctcaa tctgaagata aagctaacat
     4561 tttggataag gacgaacact tctacttagt tttcgaagaa tgttacttcg aactagctaa
     4621 catcgtccca ttgtacaaca agatcagaaa ctacattact caaaaaccat actctgatga
     4681 aaagttcaag ttaaacttcg aaaattctac cttggctaac ggttgggaca agaacaaaga
     4741 accagacaac accgccatct tgttcattaa ggacgacaag tactacttgg gtgtcatgaa
     4801 caaaaagaac aataagattt tcgacgacaa agctatcaag gagaacaagg gtgaagggta
     4861 caagaagatt gtttataagt tgttgccagg tgctaacaaa atgttgccaa aggttttctt
     4921 ctccgctaag tctatcaagt tctataaccc ttctgaagac attttgagaa tcagaaacca
     4981 ctccacccac accaagaacg gttctccaca aaagggttac gaaaagttcg aattcaacat
     5041 cgaagactgt agaaagttca tcgatttcta caagcaatcc atttccaagc atccagaatg
     5101 gaaggatttc ggtttcagat tctctgacac tcaaagatac aactccattg atgaattcta
     5161 cagagaagtc gaaaaccaag gttacaagtt gactttcgaa aacatctctg aatcttacat
     5221 tgattccgtc gttaaccaag gtaagttgta cttgttccaa atctacaaca aggacttctc
     5281 cgcctactcc aagggtagac caaacttgca caccttgtac tggaaggctt tgtttgacga
     5341 aagaaacttg caagatgttg tctacaagct gaacggtgaa gctgaattgt tttacagaaa
     5401 gcaaagtatt ccaaagaaaa tcactcaccc agctaaggaa gccattgcca acaagaataa
     5461 agacaaccct aagaaggaat ctgtttttga atacgactta atcaaggata agagattcac
     5521 cgaagacaaa ttcttcttcc attgtccaat caccatcaac ttcaagtcct ctggagctaa
     5581 caagttcaac gatgaaatca acttgttatt gaaggaaaag gctaacgatg ttcacatctt
     5641 gtctatcgat cgtggtgaaa gacacttggc ttactacact ttggttgatg gtaagggtaa
     5701 catcattaag caagacacct ttaacattat cggtaacgac agaatgaaga ccaactacca
     5761 cgacaaattg gctgctattg aaaaggacag agactctgct agaaaggact ggaaaaagat
     5821 caataacatc aaggaaatga aggaaggtta cttgtctcaa gttgtccatg aaattgctaa
     5881 gttggttatc gaatacaatg ctatcgttgt cttcgaggat ttgaacttcg gttttaagag
     5941 aggtagattc aaggttgaaa agcaagttta ccaaaaattg gaaaagatgt tgattgaaaa
     6001 gttgaactac ttagtcttca aggacaatga atttgacaag actggtggtg tcttgagagc
     6061 ttaccaattg actgctccat tcgaaacttt caagaagatg ggtaagcaaa ccggtatcat
     6121 ctactacgtt ccagctggtt tcacttctaa aatctgtcca gttaccggtt tcgtcaacca
     6181 attgtaccca aagtacgaat ccgtttccaa gtcccaagaa tttttctcca agttcgacaa
     6241 gatctgttac aacttagaca agggttattt cgagttttcc ttcgattaca aaaactttgg
     6301 tgacaaagcc gctaagggta aatggactat cgcttctttc ggttctagat tgatcaactt
     6361 ccgtaactcc gataagaacc acaactggga cactagagaa gtttacccaa ccaaggaatt
     6421 agaaaaattg ttgaaggact actctattga atacggtcac ggtgaatgta tcaaggctgc
     6481 catctgtggt gaatctgata agaagttctt cgctaagcta acttccgtct tgaacaccat
     6541 tttgcaaatg agaaactcca agaccggtac tgaattggac tacttgattt ctccagttgc
     6601 cgatgttaac ggtaacttct tcgactctag acaagctcca aagaacatgc cacaagacgc
     6661 tgatgctaac ggtgcctacc acattggttt gaagggtttg atgttgttgg gtcgtattaa
     6721 gaacaaccaa gaaggtaaga agttgaacct agtcattaag aacgaagaat acttcgaatt
     6781 tgttcaaaac agaaacaact ccagagctga cccaaagaag aagagaaaag tctaaaggag
     6841 ttaaaggcaa agttttcttt tctagagccg ttcccacaaa taattatacg tatatgcttc
     6901 ttttcgttta ctatatatct atatttacaa gcctttattc actgatgcaa tttgtttcca
     6961 aatacttttt tggagatctc ataactagat atcatgatgg cgcaacttgg cgctatctta
     7021 attactctgg ctgccaggcc cgtgtagagg gccgcaagac cttctgtacg ccatatagtc
     7081 tctaagaact tgaacaagtt tctagaccta ttgccgcctt tcggatcgct attgttgcgg
     7141 ccgccagctg aagcttcgta cgctgcaggt cgacgaattc taccgttcgt ataatgtatg
     7201 ctatacgaag ttatagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
     7261 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
     7321 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
     7381 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc
     7441 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
     7501 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
     7561 tgctaggata cagttctcac atcacatccg aacataaaca accatgggta aggaaaagac
     7621 tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     7681 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
     7741 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     7801 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     7861 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa cagcattcca
     7921 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     7981 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     8041 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     8101 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt
     8161 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     8221 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     8281 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     8341 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     8401 tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt
     8461 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt
     8521 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca
     8581 tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc
     8641 catccagtgt cgaaaacgag ctcataactt cgtataatgt atgctatacg aacggtagaa
     8701 ttcgaatcag atccactagt ggcctatgcg gccgccaccg cggtggagct ccagcttttg
     8761 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     8821 gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa
     8881 agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc
     8941 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9001 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9061 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9361 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9421 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9481 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9541 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9601 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9661 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9721 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10861 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10921 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa
    10981 aataattata atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa
    11041 agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg
    11101 ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt
    11161 tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc
    11221 gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag
    11281 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact
    11341 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca
    11401 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg
    11461 caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    11521 tcacgaggcc ctttcgtc
//
