LOCUS       sequence-19237-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf160
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             370..411
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             421..438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(461..478)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    467..693
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcttgggtac cgagctcgga tcagtaccct tcaccgaatt ccagctgacc accatgaaac
       61 acagattgta ttctgaagga ttgagtatta gtaatgattt aaactcgata atcggtcaac
      121 aatctacaat ggatacggat atagaaatag acgaagatga catcatggaa cttcttaata
      181 tattgactga gttaggttgt gatgtcgact ttgatgaaaa ttttagcgat atagccgatg
      241 atattctaga atcgttgata gaacaggatg tataagcaat tcccggggat ccgaataaag
      301 ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
      361 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
      421 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt
      481 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      541 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      601 tctattctgg gggggtgggg gtggggcagg acagcaaggg ggaggattgg gaagaccata
      661 gcaggcatgc tggggatgcg gtgggctcta tggcttctg
//
