LOCUS       sequence-19238-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf161
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             602..643
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             653..670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(693..710)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 taccctttac cgaatttcca gctgaccacc atgaggagta ttgcggggct acataaatta
       61 aaaatggaaa tttttaatgt agaagaattg ataaatatga aaccttttaa gaatatgaat
      121 aaaataacaa ttaatcaaaa ggataattgt atattagcaa atcgatgctt tgttaaaata
      181 gatactccta gatacatacc atcgacatcc attagcagtt ctaatatcat cagaatacgg
      241 aatcatgatt ttacattatc tgaattattg tattcaccgt ttcattttca acagcctcag
      301 tttcaatatc tccttcctgg gtttgtatta acgtgtattg ataaagtttc gaaacagcaa
      361 aaaaaatgta aatattgtat ctctaatcgt ggagatgatg atagtttaag cattaatcta
      421 tttattccga ctattaacaa gtctatatat attattatcg gtttacggat gaaaaatttt
      481 tggaagccta aattcgaaat agaataagca attcccgggg atccgaataa agggtcaaga
      541 caattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
      601 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
      661 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
      721 atctgttgtt t
//
