LOCUS       sequence-192408-       10749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Isl1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Isl1
FEATURES             Location/Qualifiers
     source          1..10749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    265..283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     305..329
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    402..426
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1231..1240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1511..1535)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1605..2066
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1661..1678)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             2071..2781
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2216..2234)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2484..2503
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2814..3402
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2867..2887)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3285..3296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3405..3421)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3930..4110
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4136..4153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4142..4366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4412..4840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4499..4518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4709..4730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4849..4869)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4854..5183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5034..5169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5096..5115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5231..5278
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5297..5671
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5801..5922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5838..5857)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5892..5911
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5971..5987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5971..5987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5984..6006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5995..6011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6019..6025,6026..6043,6044..6049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6064..6085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6202..6219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6373..6961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6453..6472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7132..7923,7924..7992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7755..7774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7993..8097)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8172..8191)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8363..8742
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8744..8942
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8897..8917
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8960..9140
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9187..9312
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9809..10042
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10227..10271
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10569..10686
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag
       61 aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc
      121 tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca
      181 cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga
      241 tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacc ccctatataa
      301 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      361 tccatagaag acaccgggac cgatccagcc tgctagcatc aacaagtttg tacaaaaaag
      421 caggctccga attcgccctt atgggagaca tgggcgatcc accaaaaaaa aaacgtctga
      481 tttccctgtg tgttggttgc ggcaatcaaa ttcacgacca gtatattctg agggtttctc
      541 cggatttgga gtggcatgca gcatgtttga aatgtgcgga gtgtaatcag tatttggacg
      601 aaagctgtac gtgctttgtt agggatggga aaacctactg taaaagagat tatatcaggt
      661 tgtacgggat caaatgcgcc aagtgcagca taggcttcag caagaacgac ttcgtgatgc
      721 gcgcccgctc taaggtgtac cacatcgagt gtttccgctg tgtagcctgc agccgacagc
      781 tcatcccggg agacgaattc gccctgcggg aggatgggct tttctgccgt gcagaccacg
      841 atgtggtgga gagagccagc ctgggagctg gagaccctct cagtcccttg catccagcgc
      901 ggcctctgca aatggcagcc gaacccatct cggctaggca gccagctctg cggccgcacg
      961 tccacaagca gccggagaag accacccgag tgcggactgt gctcaatgag aagcagctgc
     1021 acaccttgcg gacctgctat gccgccaacc ctcggccaga tgcgctcatg aaggagcaac
     1081 tagtggagat gacgggcctc agtcccagag tcatccgagt gtggtttcaa aacaagcggt
     1141 gcaaggacaa gaaacgcagc atcatgatga aacagctcca gcagcagcaa cccaacgaca
     1201 aaactaatat ccaggggatg acaggaactc ccatggtggc tgctagtccg gagagacatg
     1261 atggtggttt acaggctaac ccagtagagg tgcaaagtta ccagccgccc tggaaagtac
     1321 tgagtgactt cgccttgcaa agcgacatag atcagcctgc ttttcagcaa ctggtcaatt
     1381 tttcagaagg aggaccaggc tctaattcta ctggcagtga agtagcatcg atgtcctctc
     1441 agctcccaga tacacccaac agcatggtag ccagtcctat tgaggcatga ggaacataag
     1501 ggcgaattcg acccagcttt cttgtacaaa gtggttgatg cggccgcttc gagcagacat
     1561 gagtcgacgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     1621 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     1681 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     1741 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     1801 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     1861 agttggatag ttgtggaaag agtcaaatgg ctcacctcaa gcgtattcaa caaggggctg
     1921 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     1981 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     2041 ttttcctttg aaaaacacga tgataatacc atggtgagca agggcgagga ggataacatg
     2101 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     2161 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2221 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2281 atgtacggct ccaaggccta cgggaagcac cccgccgaca tccccgacta cttgaagctg
     2341 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2401 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2461 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2521 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     2581 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     2641 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     2701 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     2761 atggacgagc tgtacaagta agtcgaccat aattcgatat caagcttatc gataatcaac
     2821 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2881 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2941 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3001 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3061 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3121 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3181 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3241 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3301 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3361 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     3421 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     3481 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     3541 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3601 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     3661 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     3721 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     3781 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     3841 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     3901 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     3961 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4021 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4081 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     4141 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4201 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4261 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4321 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4381 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4441 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4501 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4561 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4621 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4681 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4741 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4801 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4861 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4921 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4981 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5041 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5101 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5161 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5221 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     5281 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     5341 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     5401 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     5461 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     5521 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     5581 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     5641 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     5701 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5761 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5881 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5941 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6001 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6061 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6121 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6181 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6781 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7261 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7381 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7441 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7501 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7561 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7621 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7681 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7741 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7801 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7861 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7921 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7981 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8041 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8101 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     8161 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     8221 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     8281 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     8341 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     8401 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8701 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     8761 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     8821 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     8881 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8941 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9001 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9061 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9121 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9181 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9241 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9301 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9361 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9421 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9481 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9541 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9601 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     9661 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     9721 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9781 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9841 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9901 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     9961 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10021 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10081 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10141 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10201 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10261 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10321 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10381 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10441 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10501 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
    10561 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10621 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10681 aattttcggg tttattacag ggacagcaga gatccagttt ggttacgcgt ccccgaaaag
    10741 tgccacctg
//
