LOCUS       sequence-192414-       10415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_GFP
FEATURES             Location/Qualifiers
     source          1..10415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    115..133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     303..327
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    400..424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(442..444,445..447,448..1161)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(487..508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(748..767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1095..1116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1175..1199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1269..1730
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1325..1342)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1735..2445
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1880..1898)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2148..2167
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2478..3066
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2531..2551)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2949..2960)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3069..3085)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3594..3774
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3800..3817)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3806..4030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4076..4504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4163..4182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4373..4394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4513..4533)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4518..4847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4698..4833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4760..4779
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4895..4942
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4961..5335
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5465..5586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5502..5521)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5556..5575
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5635..5651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5635..5651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5648..5670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5659..5675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5683..5689,5690..5707,5708..5713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5728..5749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5866..5883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6037..6625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6117..6136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6796..7587,7588..7656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7419..7438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7657..7761)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7836..7855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8027..8406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8408..8606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8561..8581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8624..8804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8851..8976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9473..9706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9891..9935
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10233..10350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa
       61 ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta
      121 tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga agtcgacacg
      181 tctcgagctc cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata
      241 gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacccc ctatataagc
      301 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      361 catagaagac accgggaccg atccagcctg ctagcatcaa caagtttgta caaaaaagca
      421 ggctccgaat tcgcccttac catggtgagc aagggcgagg agctgttcac cggggtggtg
      481 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag
      541 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      601 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      661 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      721 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      781 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      841 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      901 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
      961 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1021 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1081 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1141 atggacgagc tgtacaagta aaagggcgaa ttcgacccag ctttcttgta caaagtggtt
     1201 gatgcggccg cttcgagcag acatgagtcg acgttatttt ccaccatatt gccgtctttt
     1261 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     1321 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     1381 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     1441 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     1501 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctcacc
     1561 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     1621 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag
     1681 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa taccatggtg
     1741 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     1801 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     1861 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     1921 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgggaa gcaccccgcc
     1981 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     2041 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     2101 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     2161 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     2221 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     2281 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     2341 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     2401 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagtcga ccataattcg
     2461 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2521 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2581 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2641 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2701 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2761 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2821 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2881 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2941 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3001 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3061 ccccgcatcg ataccgtcga cctcgtcgag acctagaaaa acatggagca atcacaagta
     3121 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     3181 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     3241 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3301 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     3361 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     3421 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     3481 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     3541 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     3601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     3781 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3841 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     3901 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     3961 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4021 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4501 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4861 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     4921 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     4981 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     5041 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     5101 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     5161 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     5221 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     5281 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     5341 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5401 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5461 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5521 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5581 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5641 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5701 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5761 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5821 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5881 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5941 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6001 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6061 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7321 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7501 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7681 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7741 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     7801 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7861 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     7921 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     7981 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     8041 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     8101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     8161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     8221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     8281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     8341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     8401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     8461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     8521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     8581 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     8641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8821 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8881 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8941 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9001 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9061 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9121 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9181 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9241 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9301 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9361 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9421 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9481 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9541 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     9601 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     9661 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     9721 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     9781 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     9841 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     9901 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     9961 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10021 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10081 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10141 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
    10201 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    10261 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    10321 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    10381 gtttggttac gcgtccccga aaagtgccac ctgac
//
