LOCUS       sequence-192420-       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Nkx2.5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Nkx2.5
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1116..1140)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1210..1671
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1266..1283)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1676..2386
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1821..1839)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2089..2108
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2419..3007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2472..2492)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2890..2901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3010..3026)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3535..3715
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3741..3758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3747..3971
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4017..4445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4104..4123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4314..4335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4454..4474)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4459..4788
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4639..4774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4701..4720
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4836..4883
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4902..5276
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5406..5527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5443..5462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5497..5516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5576..5592)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5576..5592)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5589..5611)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5600..5616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5624..5630,5631..5648,5649..5654))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5669..5690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5807..5824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5978..6566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6058..6077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6737..7528,7529..7597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7360..7379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7598..7702)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7777..7796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7968..8347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8349..8547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8502..8522
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8565..8745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8792..8917
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9414..9647
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9832..9876
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10174..10291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10360..10378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10397..10415
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10434..10452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10471..10489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10508..10526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10545..10563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10582..10600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10619..10637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgggaccg atccagcctg ctagcatcaa caagtttgta caaaaaagca
      121 ggctccgaat tcgcccttcc accatgttcc ccagccctgc gctcacaccc acgcctttct
      181 cagtcaaaga catcctgaac ctggagcagc agcagcgtag cctggcgtct ggggacctgt
      241 ctgcgcgcct cgaggccacc ctggcccctg cctcctgcat gctggccgcc ttcaagcccg
      301 aggcctactc tggccccgag gcggcagcgt ccggcctggc agagctgcgc gcggagatgg
      361 gccccgcgcc ttcgcccccc aagtgctctc ctgctttccc agccgccccc acattttacc
      421 cgggagccta cggtgaccct gacccagcca aagaccctcg ggcggataaa aaagagctgt
      481 gcgcgctgca gaaggcagtg gagctggaca aagccgagac ggatggcgcc gagagaccac
      541 gcgcacggcg gcgacggaag ccacgcgtgc tcttctcgca ggcgcaggtc tacgagctgg
      601 agcggcgctt caagcaacag cggtacctgt cggcgccaga gcgcgaccag ctggccagcg
      661 tgctgaagct cacgtccacg caggtcaaga tctggttcca gaaccgtcgc tacaagtgca
      721 agcgacagcg gcaggaccag actctggagc ttctggggcc gccgccgccg cccgcgcgca
      781 ggatcgcggt gcccgtgctg gtgcgcgacg ggaagccctg cctgggggac cccgcggcct
      841 acgctcccgc ctacggcgtg ggtctcaatg cctatggcta caacgcctac ccctacccca
      901 gctacggcgg cgcggcctgc agtcccggct acagctgcgc cgcctacccc gctgcgcccc
      961 ccgccgcgca gccccccgcc gcctccgcca acagcaactt cgtgaacttt ggcgtcgggg
     1021 acttgaacac cgtgcagagt cccgggatgc cgcagggcaa ttcgggcgtc tccacgctgc
     1081 acggcatccg agcctggtag ggaagggcga attcgaccca gctttcttgt acaaagtggt
     1141 tgatgcggcc gcttcgagca gacatgagtc gacgttattt tccaccatat tgccgtcttt
     1201 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     1261 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     1321 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     1381 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     1441 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctcac
     1501 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     1561 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     1621 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatggt
     1681 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     1741 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1801 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1861 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacggga agcaccccgc
     1921 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1981 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     2041 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     2101 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     2161 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     2221 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     2281 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     2341 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagtcg accataattc
     2401 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2461 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2521 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2581 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2641 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2701 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2761 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2821 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2881 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2941 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3001 tccccgcatc gataccgtcg acctcgtcga gacctagaaa aacatggagc aatcacaagt
     3061 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     3121 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     3181 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3241 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     3301 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     3361 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     3421 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     3481 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     3541 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3601 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3661 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     3721 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3781 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3841 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3901 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3961 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4441 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4741 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4801 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4861 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     4921 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     4981 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     5041 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     5101 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     5161 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     5221 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     5281 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5341 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5401 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5461 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5521 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5581 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5641 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5701 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5761 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5821 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5881 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5941 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6001 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6061 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6661 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6721 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6781 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6841 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6901 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6961 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7021 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7081 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7141 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7201 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7261 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7321 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7381 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7441 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7501 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7561 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7741 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7801 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7861 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     7921 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     7981 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     8041 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8101 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8161 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8221 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8281 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8341 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8401 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8461 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8521 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     8581 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8641 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8701 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8761 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8821 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8881 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8941 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9001 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9061 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9121 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9181 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9241 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9301 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9361 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9421 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9481 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9541 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9601 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9661 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9721 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9781 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9841 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9901 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9961 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    10021 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    10081 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
    10141 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
    10201 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    10261 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    10321 agtttggtta cgcgtccccg aaaagtgcca cctgacgtct ccctatcagt gatagagaag
    10381 tcgacacgtc tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat
    10441 cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta
    10501 gaacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag
    10561 agaaggtacg tctagaacgt ctccctatca gtgatagaga agtcgacacg tctcgagctc
    10621 cctatcagtg atagagaagg taccccctat ataagc
//
