LOCUS       sequence-192423-       11038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Gata4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Gata4
FEATURES             Location/Qualifiers
     source          1..11038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1498..1522)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1592..2053
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1648..1665)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             2058..2768
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2203..2221)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2471..2490
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2801..3389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2854..2874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3272..3283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3392..3408)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3917..4097
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4123..4140)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4129..4353
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4399..4827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4486..4505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4696..4717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4836..4856)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4841..5170
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5021..5156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5083..5102
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5218..5265
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5284..5658
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5788..5909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5825..5844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5879..5898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5958..5974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5958..5974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5971..5993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5982..5998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6006..6012,6013..6030,6031..6036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6051..6072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6189..6206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6360..6948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6440..6459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7119..7910,7911..7979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7742..7761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7980..8084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8159..8178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8350..8729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8731..8929
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8884..8904
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8947..9127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9174..9299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9796..10029
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10214..10258
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10556..10673
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10742..10760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10779..10797
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10816..10834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10853..10871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10890..10908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10927..10945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10964..10982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11001..11019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgggaccg atccagcctg ctagcatcaa caagtttgta caaaaaagca
      121 ggctccgaat tcgccctttc agagcttggg gcgatgtacc aaagcctggc catggccgcc
      181 aaccacggcc ccccgcccgg cgcctacgaa gcaggtggcc ctggcgcctt catgcacagc
      241 gcgggcgccg cgtcctcgcc cgtctacgtg cccactccgc gggtgccgtc ctctgtgctg
      301 ggcctgtcct acctgcaggg cggtggcagt gccgctgcag ctggaaccac ctcgggtggc
      361 agctccgggg ccggcccgtc gggtgcaggg cctgggaccc agcagggtag ccctggctgg
      421 agccaagctg gagccgaggg agccgcctac accccgccgc ccgtgtcccc gcgcttctct
      481 ttcccgggga ctactgggtc cctggcggcc gctgccgccg ctgccgcagc ccgggaagct
      541 gcagcctacg gcagtggcgg cggggcggcg ggcgctggtc tggctggccg agagcagtac
      601 gggcgtccgg gcttcgccgg ctcctactcc agcccctacc cagcctacat ggccgacgtg
      661 ggagcatcct gggccgcagc cgctgccgcc tctgccggcc ccttcgacag cccagtcctg
      721 cacagcctgc ctggacgggc caaccctgga agacacccca atctcgatat gtttgatgac
      781 ttctcagaag gcagagagtg tgtcaattgt ggggccatgt ccaccccact ctggaggcga
      841 gatgggacgg gacactacct gtgcaatgcc tgtggcctct atcacaagat gaacggcatc
      901 aaccggcccc tcattaagcc tcagcgccgc ctgtccgctt cccgccgggt aggcctctcc
      961 tgtgccaact gccagactac caccaccacg ctgtggcgtc gtaatgccga gggtgagcct
     1021 gtatgtaatg cctgcggcct ctacatgaag ctccatgggg ttcccaggcc tcttgcaatg
     1081 cggaaggagg ggattcaaac cagaaaacgg aagcccaaga acctgaataa atctaagacg
     1141 ccagcaggtc ctgctggtga gaccctccct ccctccagtg gtgcctccag cggtaactcc
     1201 agcaatgcca ctagcagcag cagcagcagt gaagagatgc gccccatcaa gacagagccc
     1261 gggctgtcat ctcactatgg gcacagcagc tccatgtccc agacattcag tactgtgtcc
     1321 ggccacgggc cctccatcca tccagtgctg tctgctctga agctgtcccc acaaggctat
     1381 gcatctcctg tcactcagac atcgcaggcc agctccaagc aggactcttg gaacagcctg
     1441 gtcctggctg acagtcatgg ggacataatc accgcgtaat cagcaagggc gaattcgacc
     1501 cagctttctt gtacaaagtg gttgatgcgg ccgcttcgag cagacatgag tcgacgttat
     1561 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     1621 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     1681 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     1741 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     1801 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     1861 tggaaagagt caaatggctc acctcaagcg tattcaacaa ggggctgaag gatgcccaga
     1921 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     1981 agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     2041 aacacgatga taataccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     2101 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     2161 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2221 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     2281 aggcctacgg gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     2341 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     2401 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     2461 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     2521 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     2581 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2641 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     2701 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2761 acaagtaagt cgaccataat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     2821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3181 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3361 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgtc gagacctaga
     3421 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     3481 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     3541 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3601 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     3661 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     3721 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     3781 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     3841 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     3901 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3961 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4021 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4081 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4141 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4201 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4261 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4321 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4381 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4441 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4501 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4561 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4621 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4681 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4741 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4801 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4861 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4921 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4981 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5041 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5101 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5161 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     5221 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     5281 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     5341 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     5401 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     5461 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     5521 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     5581 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     5641 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     5701 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5761 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5881 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8101 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     8161 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     8221 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     8281 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     8341 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     8401 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     8461 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     8521 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     8581 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     8641 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     8701 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     8761 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     8821 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     8881 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
     8941 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9001 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9061 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9121 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9181 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9241 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     9301 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     9361 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9421 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9481 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9541 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9601 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     9661 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     9721 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9781 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9841 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9901 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9961 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    10021 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10081 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10141 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10201 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10261 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10321 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10381 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    10441 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10501 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
    10561 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    10621 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    10681 attacaggga cagcagagat ccagtttggt tacgcgtccc cgaaaagtgc cacctgacgt
    10741 ctccctatca gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg
    10801 tacgtctaga acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc
    10861 agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc
    10921 tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga
    10981 gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtaccccct atataagc
//
