LOCUS       sequence-192426-       11126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Baf60c in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Baf60c
FEATURES             Location/Qualifiers
     source          1..11126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1229..1253)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1323..1784
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1379..1396)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1789..2499
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1934..1952)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2202..2221
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2532..3120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2585..2605)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3003..3014)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3123..3139)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3648..3828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3854..3871)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3860..4084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4130..4558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4217..4236)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4427..4448
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4567..4587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4572..4901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4752..4887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4814..4833
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4949..4996
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5015..5389
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5519..5640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5556..5575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5610..5629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5689..5705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5689..5705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5702..5724)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5713..5729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5737..5743,5744..5761,5762..5767))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5782..5803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5920..5937)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6091..6679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6171..6190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6850..7641,7642..7710))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7473..7492
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7711..7815)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7890..7909)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8081..8460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8462..8660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8615..8635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8678..8858
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8905..9030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9527..9760
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9945..9989
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10287..10404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10473..10491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10510..10528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10547..10565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10584..10602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10621..10639
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10658..10676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10695..10713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10732..10750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10772..10796
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10869..10893
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagccggtgc ccaccgcccc cgcgcggagc cgcagtgcca agaggaggaa gatggctgac
       61 aaaatcctcc ctcaaaggat tcgggagctg gtccccgagt cccaggctta catggacctc
      121 ttggcatttg agaggaaact ggatcaaacc atcatgcgga agcgggtgga catccaggag
      181 gctctgaaga ggcccatgaa gcaaaagcgg aagctgcgac tctatatctc caacactttt
      241 aaccctgcga agcctgatgc tgaggattcc gacggcagca ttgcctcctg ggagctacgg
      301 gtggagggga agctcctgga tgatcccagc aaacagaagc ggaagttctc ttctttcttc
      361 aagagtttgg tcatcgagct ggacaaagat ctttatggcc ctgacaacca cctcgttgag
      421 tggcatcgga cacccacgac ccaggagacg gacggcttcc aggtgaaacg gcctggggac
      481 ctgagtgtgc gctgcacgct gctcctcatg ctggactacc agcctcccca gttcaaactg
      541 gatccccgcc tagcccggct gctggggctg cacacacaga gccgctcagc cattgtccag
      601 gccctgtggc agtatgtgaa gaccaacagg ctgcaggact cccatgacaa ggaatacatc
      661 aatggggaca agtatttcca gcagattttt gattgtcccc ggctgaagtt ttctgagatt
      721 ccccagcgcc tcacagccct gctattgccc cctgacccaa ttgtcatcaa ccatgtcatc
      781 agcgtggacc cttcagacca gaagaagacg gcgtgctatg acattgacgt ggaggtggag
      841 gagccattaa aggggcagat gagcagcttc ctcctatcca cggccaacca gcaggagatc
      901 agtgctctgg acagtaagat ccatgagacg attgagtcca taaaccagct caagatccag
      961 agggacttca tgctaagctt ctccagagac cccaaaggct atgtccaaga cctgctccgc
     1021 tcccagagcc gggacctcaa ggtgatgaca gatgtagccg gcaaccctga agaggagcgc
     1081 cgggctgagt tctaccacca gccctggtcc caggaggccg tcagtcgcta cttctactgc
     1141 aagatccagc agcgcaggca ggagctggag cagtcgctgg ttgtgcgcaa cacctaggag
     1201 cccaaaaaca agcagaaggg cgaattcgac ccagctttct tgtacaaagt ggttgatgcg
     1261 gccgcttcga gcagacatga gtcgacgtta ttttccacca tattgccgtc ttttggcaat
     1321 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     1381 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     1441 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     1501 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     1561 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct cacctcaagc
     1621 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     1681 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc
     1741 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggtgagcaag
     1801 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1861 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1921 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1981 atcctgtccc ctcagttcat gtacggctcc aaggcctacg ggaagcaccc cgccgacatc
     2041 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2101 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2161 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2221 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2281 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2341 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     2401 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2461 cgccactcca ccggcggcat ggacgagctg tacaagtaag tcgaccataa ttcgatatca
     2521 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2581 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2641 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2701 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2761 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2821 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2881 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2941 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3001 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3061 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3121 atcgataccg tcgacctcgt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     3181 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     3241 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     3301 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3361 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     3421 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     3481 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     3541 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     3601 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     3661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     3841 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3901 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3961 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4021 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4081 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4321 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4441 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4561 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4621 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4681 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4801 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4861 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4921 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     4981 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     5041 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     5101 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     5161 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     5221 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     5281 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     5341 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     5401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5641 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7861 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7921 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     7981 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     8041 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     8101 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     8161 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8221 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8281 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8341 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8401 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8461 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8521 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8581 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8641 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     8701 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8761 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8881 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8941 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9001 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9061 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9121 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9181 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9241 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9301 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9361 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     9421 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     9481 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     9541 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     9601 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     9661 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     9721 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     9781 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     9841 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     9901 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     9961 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    10021 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    10081 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    10141 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
    10201 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
    10261 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    10321 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    10381 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    10441 ttacgcgtcc ccgaaaagtg ccacctgacg tctccctatc agtgatagag aagtcgacac
    10501 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
    10561 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc
    10621 tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt
    10681 acgtctagaa cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca
    10741 gtgatagaga aggtaccccc tatataagca gagctcgttt agtgaaccgt cagatcgcct
    10801 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctgc
    10861 tagcatcaac aagtttgtac aaaaaagcag gctccgaatt cgcccttacc atgactccag
    10921 gtcttcagca cccacccacc gtggtacagc gccccgggat gccgtctgga gcccggatgc
    10981 cccaccaggg ggcgcccatg ggccccccgg gctccccgta catgggcagc cccgccgtgc
    11041 gacccggcct ggcccccgcg ggcatggagc ccgcccgcaa gcgagcagcg cccccgcccg
    11101 ggcagagcca ggcacagagc cagggc
//
