LOCUS       sequence-192429-       10511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Mesp1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Mesp1
FEATURES             Location/Qualifiers
     source          1..10511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        514..712
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             730..910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    957..1082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1579..1812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1997..2041
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2339..2456
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2525..2543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2562..2580
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2599..2617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2636..2654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2673..2691
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2710..2728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2747..2765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2784..2802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2921..2945
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3792..3816)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3886..4347
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3942..3959)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4352..5062
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4497..4515)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4765..4784
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5095..5683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5148..5168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5566..5577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5686..5702)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6211..6391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6417..6434)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6423..6647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6693..7121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6780..6799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6990..7011
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7130..7150)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7135..7464
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7315..7450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7377..7396
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7512..7559
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7578..7952
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8082..8203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8119..8138)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8173..8192
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8252..8268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8252..8268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8265..8287)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8276..8292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8300..8306,8307..8324,8325..8330))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8345..8366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8483..8500)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8654..9242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8734..8753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9413..10204,10205..10273))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10036..10055
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10274..10378)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10453..10472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
       61 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
      121 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
      721 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      901 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      961 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1021 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1081 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1141 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1201 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1261 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     1321 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1381 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     1441 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1501 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1561 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1621 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1681 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1741 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1801 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1861 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1921 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1981 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2041 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2101 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2161 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2221 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2281 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2341 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2401 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2461 tttattacag ggacagcaga gatccagttt ggttacgcgt ccccgaaaag tgccacctga
     2521 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     2581 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     2641 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     2701 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     2761 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     2821 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct gctagcatca acaagtttgt acaaaaaagc
     2941 aggctccgaa ttcgcccttg ccgccgctgc catggcccag cccctgtgcc cgccgctctc
     3001 cgagtcctgg atgctctctg cggcctgggg cccaactcgg cggccgccgc cctccgacaa
     3061 ggactgcggc cgctccctcg tctcgtcccc agactcatgg ggcagcaccc cagccgacag
     3121 ccccgtggcg agccccgcgc ggccaggcac cctccgggac ccccgcgccc cctccgtagg
     3181 taggcgcggc gcgcgcagca gccgcctggg cagcgggcag aggcagagcg ccagtgagcg
     3241 ggagaaactg cgcatgcgca cgctggcccg cgccctgcac gagctgcgcc gctttctacc
     3301 gccgtccgtg gcgcccgcgg gccagagcct gaccaagatc gagacgctgc gcctggctat
     3361 ccgctatatc ggccacctgt cggccgtgct aggcctcagc gaggagagtc tccagcgccg
     3421 gtgccggcag cgcggtgacg cggggtcccc tcggggctgc ccgctgtgcc ccgacgactg
     3481 ccccgcgcag atgcagacac ggacgcaggc tgaggggcag gggcaggggc gcgggctggg
     3541 cctggtatcc gccgtccgcg ccggggcgtc ctggggatcc ccgcctgcct gccccggagc
     3601 ccgagctgca cccgagccgc gcgacccgcc tgcgctgttc gccgaggcgg cgtgcccgga
     3661 agggcaggcg atggagccaa gcccaccgtc cccgctcctt ccgggcgacg tgctggctct
     3721 gttggagacc tggatgcccc tctcgcctct ggagtggctg cctgaggagc ccaagtgaaa
     3781 gggcgaattc gacccagctt tcttgtacaa agtggttgat gcggccgctt cgagcagaca
     3841 tgagtcgacg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3901 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3961 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4021 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4081 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4141 gagttggata gttgtggaaa gagtcaaatg gctcacctca agcgtattca acaaggggct
     4201 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4261 ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     4321 gttttccttt gaaaaacacg atgataatac catggtgagc aagggcgagg aggataacat
     4381 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     4441 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4501 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     4561 catgtacggc tccaaggcct acgggaagca ccccgccgac atccccgact acttgaagct
     4621 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4681 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4741 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4801 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4861 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4921 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4981 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     5041 catggacgag ctgtacaagt aagtcgacca taattcgata tcaagcttat cgataatcaa
     5101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     5701 cgtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     5761 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     5821 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5881 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     5941 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6001 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     6061 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     6121 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     6181 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     6241 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6301 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6361 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     6421 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6481 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6541 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6601 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6661 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6841 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6901 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6961 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7021 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7081 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7141 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7201 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7261 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7381 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7441 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7501 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     7561 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     7621 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     7681 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     7741 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     7801 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     7861 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     7921 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     7981 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8041 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8221 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10381 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    10441 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    10501 ttgtgtgttg g
//
