LOCUS       sequence-192435-       11255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Irx4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Irx4
FEATURES             Location/Qualifiers
     source          1..11255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1637..1661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1731..2192
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1787..1804)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             2197..2907
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2342..2360)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2610..2629
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2940..3528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2993..3013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3411..3422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3531..3547)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4056..4236
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4262..4279)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4268..4492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4538..4966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4625..4644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4835..4856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4975..4995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4980..5309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5160..5295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5222..5241
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5357..5404
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5423..5797
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5927..6048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5964..5983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6018..6037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6097..6113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6097..6113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6110..6132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6121..6137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6145..6151,6152..6169,6170..6175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6190..6211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6328..6345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6499..7087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6579..6598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7258..8049,8050..8118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7881..7900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8119..8223)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8298..8317)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8489..8868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8870..9068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9023..9043
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9086..9266
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9313..9438
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9935..10168
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10353..10397
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10695..10812
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10881..10899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10918..10936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10955..10973
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10992..11010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11029..11047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11066..11084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11103..11121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11140..11158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11180..11204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgatccagcc tgctagcatc aacaagtttg tacaaaaaag caggctccga attcgccctt
       61 accatgtcct acccgcagtt tggatacccc tactcctcgg ctccccagtt cttgatggcc
      121 accaactccc tgagcacgtg ctgcgagtcc ggaggccgca cgctggcgga ctccgggccc
      181 gccgcctcgg cccaggcgcc ggtctactgc ccggtctacg agagccggct gctggccacc
      241 gcgcgccacg agctcaactc ggccgcggcg ctgggcgtct atgggggtcc ctatggcgga
      301 tcgcagggct atggcaacta cgtgacctac ggctcggagg cgtccgcctt ctactcgctg
      361 aacagctttg attccaagga tggttcggga tctgcgcatg ggggcctggc accagccact
      421 gccgcctact acccttacga gccagctctg ggccagtacc cctatgacag gtatggaacc
      481 atggacagcg gcacgcggcg caagaacgcc acgcgcgaga ccaccagcac gctcaaggcc
      541 tggctgcagg agcaccgcaa gaacccctac cccaccaagg gcgagaagat catgctggcc
      601 atcatcacca agatgaccct cacacaggtc tccacctggt tcgccaacgc gcgccggcgc
      661 ctcaagaagg agaacaagat gacgtggccg ccgcggaaca agtgcgcaga cgagaagcgg
      721 ccctacgcgg agggcgagga ggaggagggg ggcgaggagg aggcgcggga ggagcccctc
      781 aagagctcca agaacgcaga gcccgtgggc aaagaggaga aggagctgga gcttagtgac
      841 ttggacgact tcgacccgct ggaagcagag ccgccggcgt gcgagctgaa gccgcccttc
      901 cactccctgg acggcggtct ggagcgcgtc cccgccgcgc ccgacggccc ggtcaaggag
      961 gcctcaggcg cgctccggat gtctctggcc gcgggtggcg gagctgctct ggacgaggac
     1021 ctggagaggg cccggagctg tctccgcagc gcggcggccg ggccggagcc actgccgggc
     1081 gcagagggcg gccctcaggt ctgcgaggcc aagctggggt ttgtgccggc gggggcgtcg
     1141 gcaggcctgg aggctaagcc gcgcatctgg tccctggccc acacagccac cgccgccgcc
     1201 gccgccgcca cctccctgag ccagactgag tttccgtcgt gcatgctcaa gcgccaaggt
     1261 cccgcggccc ctgcggctgt gtcctccgcg cccgccacgt ccccgtctgt ggcccttccc
     1321 cactctggcg ccctggacag gcaccaggac tccccggtaa ccagtctcag aaactgggtg
     1381 gacggggtct tccacgaccc catcctcagg cacagcactt tgaaccaggc ctgggccacc
     1441 gccaagggcg ccctcctgga ccccgggcct ctgggacgct cgctgggggc gggcgcgaac
     1501 gtgctgactg cacccctggc ccgcgccttt ccgcctgccg tgccccagga cgccccagct
     1561 gcaggcgccg ccagggagct gctcgccctg cccaaggccg gcggcaaacc cttctgcgcc
     1621 tgaaagggcg aattcgaccc agctttcttg tacaaagtgg ttgatgcggc cgcttcgagc
     1681 agacatgagt cgacgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     1741 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     1801 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     1861 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     1921 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     1981 gttgtgagtt ggatagttgt ggaaagagtc aaatggctca cctcaagcgt attcaacaag
     2041 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     2101 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
     2161 acgtggtttt cctttgaaaa acacgatgat aataccatgg tgagcaaggg cgaggaggat
     2221 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     2281 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2341 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     2401 cagttcatgt acggctccaa ggcctacggg aagcaccccg ccgacatccc cgactacttg
     2461 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     2521 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     2581 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     2641 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     2701 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     2761 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     2821 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     2881 ggcggcatgg acgagctgta caagtaagtc gaccataatt cgatatcaag cttatcgata
     2941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3301 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3481 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3541 gacctcgtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     3601 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     3661 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3721 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     3781 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     3841 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     3901 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     3961 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     4021 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     4081 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4141 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4201 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     4261 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4321 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4381 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4441 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4501 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4741 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4861 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4981 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5041 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5101 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5221 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5281 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5341 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     5401 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     5461 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     5521 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     5581 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     5641 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     5701 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     5761 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     5821 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5881 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5941 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6001 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     6061 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6121 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6181 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6241 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6301 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6901 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7141 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7201 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7261 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7321 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7381 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7441 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7501 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7561 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7621 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7681 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7741 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7801 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7861 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7921 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7981 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8041 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8101 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8161 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8221 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     8281 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     8341 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     8401 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     8461 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     8521 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     8581 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     8641 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     8701 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     8761 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     8821 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     8881 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     8941 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     9001 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     9061 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     9121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9241 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9301 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9361 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     9421 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     9481 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     9541 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     9601 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     9661 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     9721 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     9781 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     9841 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9901 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9961 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    10021 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    10081 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    10141 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    10201 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    10261 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    10321 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10381 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10441 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10501 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10561 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10621 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
    10681 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10741 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10801 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt acgcgtcccc
    10861 gaaaagtgcc acctgacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
    10921 ctatcagtga tagagaaggt acgtctagaa cgtctcccta tcagtgatag agaagtcgac
    10981 acgtctcgag ctccctatca gtgatagaga aggtacgtct agaacgtctc cctatcagtg
    11041 atagagaagt cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg
    11101 tctccctatc agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag
    11161 gtacccccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
    11221 cacgctgttt tgacctccat agaagacacc gggac
//
