LOCUS       sequence-192438-       11230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of SRF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_SRF
FEATURES             Location/Qualifiers
     source          1..11230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1382..1406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1476..1937
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1532..1549)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1942..2652
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2087..2105)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2355..2374
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2685..3273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2738..2758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3156..3167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3276..3292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3801..3981
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4007..4024)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4013..4237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4283..4711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4370..4389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4580..4601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4720..4740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4725..5054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4905..5040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4967..4986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5102..5149
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5168..5542
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5672..5793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5709..5728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5763..5782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5842..5858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5842..5858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5855..5877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5866..5882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5890..5896,5897..5914,5915..5920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5935..5956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6073..6090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6244..6832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6324..6343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7003..7794,7795..7863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7626..7645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7864..7968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8043..8062)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8234..8613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8615..8813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8768..8788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8831..9011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9058..9183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9680..9913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10098..10142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10440..10557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10626..10644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10663..10681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10700..10718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10737..10755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10774..10792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10811..10829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10848..10866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10885..10903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10925..10949
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11022..11046
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcctggagcg ggaggctgcg gcagcggcgg caaccacccc ggcgcccacc gcgggggccc
       61 tctacagcgg cagcgagggc gactcggagt cgggcgagga ggaggagctg ggcgccgagc
      121 ggcgcggcct gaagcggagc ctgagcgaga tggagatcgg tatggtggtc ggtgggcccg
      181 aggcgtcggc agcggccacc gggggctacg ggccggtgag cggcgcggtg agcggggcca
      241 agccgggtaa gaagacccgg ggccgcgtga agatcaagat ggagttcatc gacaacaagc
      301 tgcggcgcta cacgaccttc agcaagagga agacgggcat catgaagaag gcctatgagc
      361 tgtccacgct gacagggaca caggtgctgt tgctggtggc cagtgagaca ggccatgtgt
      421 atacctttgc cacccgaaaa ctgcagccca tgatcaccag tgagaccggc aaggcactga
      481 ttcagacctg cctcaactcg ccagactctc caccccgttc agaccccaca acagaccaga
      541 gaatgagtgc cactggcttt gaagagacag atctcaccta ccaggtgtcg gagtctgaca
      601 gcagtgggga gaccaaggac acactgaagc cggcgttcac agtcaccaac ctgccgggta
      661 caacctccac catccaaaca gcacctagca cctctaccac catgcaagtc agcagcggcc
      721 cctcctttcc catcaccaac tacctggcac cagtgtctgc tagtgtcagc cccagtgctg
      781 tcagcagtgc caatgggact gtgctgaaga gtacaggcag cggccctgtc tcctctgggg
      841 gccttatgca gctgcctacc agcttcaccc tcatgcctgg tggggcagtg gcccagcagg
      901 tcccagtgca ggccattcaa gtgcaccagg ccccacagca agcgtctccc tcccgtgaca
      961 gcagcacaga cctcacgcag acctcctcca gcgggacagt gacgctgccc gccaccatca
     1021 tgacgtcatc cgtgcccaca actgtgggtg gccacatgat gtaccctagc ccgcatgcgg
     1081 tgatgtatgc ccccacctcg ggcctgggtg atggcagcct caccgtgctg aatgccttct
     1141 cccaggcacc atccaccatg caggtgtcac acagccaggt ccaggagcca ggtggcgtcc
     1201 cccaggtgtt cctgacagca tcatctggga cagtgcagat ccctgtttca gcagttcagc
     1261 tccaccagat ggctgtgata gggcagcagg ccgggagcag cagcaacctc accgagctac
     1321 aggtggtgaa cctggacacc gcccacagca ccaagagtga atgatccgaa gggcgaattc
     1381 gacccagctt tcttgtacaa agtggttgat gcggccgctt cgagcagaca tgagtcgacg
     1441 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     1501 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     1561 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     1621 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     1681 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     1741 gttgtggaaa gagtcaaatg gctcacctca agcgtattca acaaggggct gaaggatgcc
     1801 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     1861 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     1921 gaaaaacacg atgataatac catggtgagc aagggcgagg aggataacat ggccatcatc
     1981 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2041 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2101 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2161 tccaaggcct acgggaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2221 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2281 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2341 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     2401 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     2461 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     2521 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     2581 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     2641 ctgtacaagt aagtcgacca taattcgata tcaagcttat cgataatcaa cctctggatt
     2701 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2761 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2821 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2881 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2941 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3001 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3061 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     3121 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3181 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3241 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgtcgagacc
     3301 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     3361 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     3421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3481 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     3541 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     3601 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     3661 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     3721 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     3781 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3961 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4021 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4081 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4141 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4201 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4261 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4321 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4621 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4681 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4801 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4861 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4921 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4981 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5041 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     5101 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     5161 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     5221 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     5281 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     5341 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     5401 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     5461 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     5521 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     5581 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5641 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5701 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5761 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     5821 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5881 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5941 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6001 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6661 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7981 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     8041 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     8101 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     8161 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     8221 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     8281 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8341 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8401 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8461 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     8521 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     8581 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     8641 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     8701 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     8761 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     8821 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8881 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8941 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9001 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     9061 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9121 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9181 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9241 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9301 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9361 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9421 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9481 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     9541 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9601 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9661 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9721 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9781 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     9841 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9901 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9961 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    10021 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    10081 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    10141 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    10201 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    10261 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    10321 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    10381 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
    10441 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10501 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    10561 gtttattaca gggacagcag agatccagtt tggttacgcg tccccgaaaa gtgccacctg
    10621 acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag
    10681 aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc
    10741 tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca
    10801 cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga
    10861 tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacc ccctatataa
    10921 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
    10981 tccatagaag acaccgggac cgatccagcc tgctagcatc aacaagtttg tacaaaaaag
    11041 caggctccga attcgccctt gagcgccatg ttaccgaccc aagctggggc cgcggcggct
    11101 ctgggccggg gctcggccct ggggggcagc ctgaaccgga ccccgacggg gcggccgggc
    11161 ggcggcggcg ggacacgcgg ggctaacggg ggccgggtcc ccgggaatgg cgcggggctc
    11221 gggcccggcc
//
