LOCUS       sequence-192440-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression construct for S protein-Flag-Streptavidin 
            binding peptide (SFB)-tagged RNF169.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Chen J, Feng W, Jiang J, Deng Y, Huen MS
  TITLE     Ring finger protein RNF169 antagonizes the ubiquitin-dependent 
            signaling cascade at sites of DNA damage.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27715-22. doi: 
            10.1074/jbc.M112.373530. Epub 2012 Jun 25.
  PUBMED    22733822
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.373530"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27715-22"
COMMENT     Sequence Label: pMH-SFB-RNF169
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..82
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             116..139
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             143..166
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             176..289
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    362..386
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctagcgcta ccggactcag atctcgaggc caccatgaaa gaaaccgctg ctgctaaatt
       61 cgaacgccag cacatggaca gcggagccgg cgcaggagcc ggcgctgacg cgcctgacta
      121 caaagacgat gacgacaagg gagattacaa ggatgacgat gacaaaggcg cgtcgatgga
      181 cgagaagacc accggctggc ggggcggcca cgtggtggag ggcctggccg gcgagctgga
      241 gcagctgcgg gccaggctgg agcaccaccc tcagggccag cgggagccct ccggcggctg
      301 caagctgggc ggcgcgcctg aattctgcag tcgacggtac cgcgggccca tcgctcgaga
      361 gacaagtttg tacaaaaaag caggcttcat ggcggctgca ggtccgagta ctcgggcctc
      421 ttccgcggcg gcagcagccg ctctgagtcg gcggggccgg cggggccgct gtgacgagac
      481 ggcggcagct aagactgggg ccccaggccc ggcttctgga ccttcgctgt tggtgttgtc
      541 gccgccgttg ctgcagccgc cgctgccgcc gcggccggag gaatcgggct gcgccgggtg
      601 cctggagccc cccggagaag cagcggccct gccgtgcggc cactcgcttt gccgaggctg
      661 cgcccaacgc gccgccgacg cggcgggccc gggttgccct cgctgcc
//
