LOCUS       sequence-19245-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf168
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             355..396
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             406..423
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(446..463)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    452..678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 taccgagctc ggatccagta cccttcaccg aattccagct gaccaccatg ctacatgagt
       61 tccatgtgcg gagattgtta aagtttaggg tagagagttg ttctagcatc cattcattgt
      121 ctttacatcc gttatatgta attatgtcac ccatggacat tataggtttt aatagtactt
      181 cagatactgg aataagatat tgttcgtata tgtgtttgat aatcatttgt tgcgtttctg
      241 gagaactttt ctctgcatga gcaattcccg gggatccgaa taaagggtca agacaattct
      301 gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
      361 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
      421 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
      481 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
      541 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
      601 tgggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
      661 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtat
//
