LOCUS       sequence-192454-        2587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid vector pDGB2-alpha-2, adapted for S. cerevisiae genome 
            integration by flanking the GB cassette with recombination arms of 
            homology for integration at the YPRC-Delta-15 solo LTR loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: YPRC-Delta-15-alpha-2
FEATURES             Location/Qualifiers
     source          1..2587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1056..1379)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     1061..1080
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1274..1296
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1288..1305
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1289..1305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1306..1362
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1375..1391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1375..1391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1388..1410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1399..1415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1423..1429,1430..1447,1448..1453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1468..1489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggccgcta gggataacag ggtaatcgag atagtttgac gttcgttttt tactttgaat
       61 atactcgtag tctttttact ttttgagtat aaaaaaaaaa tgactagcaa aataaaatta
      121 gtagtctaaa aaagaaagct cgcactcagg atcgaactaa ggaccaacag atttgcaatc
      181 tgctgcgcta ccactgcgcc atacgagctt ttgaattatg gtaattttga ttatcctaga
      241 atgttatatc tcaatatctc aatatatttt ggacatctat gaaacaccca taaagcagcc
      301 gctaccaaac agacaagatt cagtatgtaa ggtaaatacc tttttgcaca gttaaactac
      361 ccaaacttat taaagcttga taaattactg aaattccacc tttcagttag attcaggcct
      421 catatagatt agatataggg tacgtaacat tctgtcaacc aagttgttgg aatgaaagtc
      481 taaaatgtca tctattcggt agcactcatg ttactagtat actgtcacat gcggtgtaac
      541 gtggggacat aaaacagaca tcaaatataa tggaagctga aatgcaaaga tcgataatgt
      601 aataggaatg aaacatataa aacgaaagga gaagtaatgg taatattagt atgtagaaat
      661 accgattcaa ttttggggat tcttatattc tcgagagaat ttctagtata atctgtatac
      721 ataatattat aggctttacc aacaatggaa tttcgacaat tatcatatta ttcaccaatt
      781 aatcacaagt tggtaatgag tttgataaca agttactttc ttaacaacgt tagtatcgtc
      841 aaaacactcg gttttactcg agcttgtagc acaataatac cgtgtagagt tctgtattgt
      901 tcttcttagt gcttgtatat gctcatcccg accttccatt aagcttcgtc tcagtcagga
      961 gagagaccca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     1021 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     1081 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     1141 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     1201 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     1261 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
     1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
     1381 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1441 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1501 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctggggtctc tcgcttgaga
     1561 cgaagctttt tgcgaaaccc tatgctctgt tgttcggatt tgaaatttta aaactacatt
     1621 aatgtgttag tttttctttc tttctttctt tgtcttgacg tgatttggac ttctgtcttg
     1681 cattcgcgtc cattcatctg acccaatatt ccttttggtt ttgttatcct tataaaaaga
     1741 aaggaagctt cttagaggga aaaaaatgat gaagagtaat gccaaaatat aaataaataa
     1801 ataaatatga aaatcatttt ctatttttaa tagaataaga agagcatctt aagattacaa
     1861 tttcaagaaa tagtttacac agtatatcca ataactccaa taaactactt tcctatacaa
     1921 atttctatgg tgggattaat agtaaaactt ctgtacttct ctaattcacc aagaaattaa
     1981 ggtaaacatc tggtaagcac tatccagctt tttgctatta cacatatggc ttttctgcaa
     2041 tcatttcttc ccattttgtc tcaagccgtt agtcttgaaa ccacaggcgg agtagagtta
     2101 cttgatgcgg tattttacat gccttttttc actgcaaaaa aaatgaaata catatttaca
     2161 cgatttgcag gacagtttac gatagtgagt atgcagaata gttaacacct ttgttttatc
     2221 cttttgtgtc ttaattatat gatataaagg cgcctggcgt tatcggatag taatagatgc
     2281 tagttatcaa catttcacaa ttgaaggaaa taaagttgaa gtactcaaca aaaacttact
     2341 tcagaattaa atttttgggg ggaacatagg catcctatga caggtgacca caagcccctc
     2401 aacgcaatct aatattttac aaagtggtaa aattctttcg ttcttcgttt taatatacag
     2461 tcatttattg attctattac attaatattc ctacgcttcg gctcacataa ttaacaggac
     2521 ttcgagtccg ttaaacttgg gatcaactaa tttctacgga ttagggataa cagggtaatg
     2581 cggccgc
//
