LOCUS       sequence-192455-        2586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid vector pDGB2-Omega-1, adapted for S. cerevisiae genome 
            integration by flanking the GB cassette with recombination arms of 
            homology for integration at the YPRC-Delta-15 solo LTR loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: YPRC-Delta-15-Omega-1
FEATURES             Location/Qualifiers
     source          1..2586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1052..1375)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     1057..1076
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1270..1292
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1284..1301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1285..1301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1302..1358
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1371..1387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1371..1387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1384..1406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1395..1411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1419..1425,1426..1443,1444..1449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1464..1485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggccgcta gggataacag ggtaatcgag atagtttgac gttcgttttt tactttgaat
       61 atactcgtag tctttttact ttttgagtat aaaaaaaaaa tgactagcaa aataaaatta
      121 gtagtctaaa aaagaaagct cgcactcagg atcgaactaa ggaccaacag atttgcaatc
      181 tgctgcgcta ccactgcgcc atacgagctt ttgaattatg gtaattttga ttatcctaga
      241 atgttatatc tcaatatctc aatatatttt ggacatctat gaaacaccca taaagcagcc
      301 gctaccaaac agacaagatt cagtatgtaa ggtaaatacc tttttgcaca gttaaactac
      361 ccaaacttat taaagcttga taaattactg aaattccacc tttcagttag attcaggcct
      421 catatagatt agatataggg tacgtaacat tctgtcaacc aagttgttgg aatgaaagtc
      481 taaaatgtca tctattcggt agcactcatg ttactagtat actgtcacat gcggtgtaac
      541 gtggggacat aaaacagaca tcaaatataa tggaagctga aatgcaaaga tcgataatgt
      601 aataggaatg aaacatataa aacgaaagga gaagtaatgg taatattagt atgtagaaat
      661 accgattcaa ttttggggat tcttatattc tcgagagaat ttctagtata atctgtatac
      721 ataatattat aggctttacc aacaatggaa tttcgacaat tatcatatta ttcaccaatt
      781 aatcacaagt tggtaatgag tttgataaca agttactttc ttaacaacgt tagtatcgtc
      841 aaaacactcg gttttactcg agcttgtagc acaataatac cgtgtagagt tctgtattgt
      901 tcttcttagt gcttgtatat gctcatcccg accttccatt ggatccggtc tcaggagtga
      961 gacgcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1021 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     1081 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1141 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1201 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1261 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc
     1321 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     1381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc gtctcacgct gtcatgagac
     1561 cggatccttt gcgaaaccct atgctctgtt gttcggattt gaaattttaa aactacatta
     1621 atgtgttagt ttttctttct ttctttcttt gtcttgacgt gatttggact tctgtcttgc
     1681 attcgcgtcc attcatctga cccaatattc cttttggttt tgttatcctt ataaaaagaa
     1741 aggaagcttc ttagagggaa aaaaatgatg aagagtaatg ccaaaatata aataaataaa
     1801 taaatatgaa aatcattttc tatttttaat agaataagaa gagcatctta agattacaat
     1861 ttcaagaaat agtttacaca gtatatccaa taactccaat aaactacttt cctatacaaa
     1921 tttctatggt gggattaata gtaaaacttc tgtacttctc taattcacca agaaattaag
     1981 gtaaacatct ggtaagcact atccagcttt ttgctattac acatatggct tttctgcaat
     2041 catttcttcc cattttgtct caagccgtta gtcttgaaac cacaggcgga gtagagttac
     2101 ttgatgcggt attttacatg ccttttttca ctgcaaaaaa aatgaaatac atatttacac
     2161 gatttgcagg acagtttacg atagtgagta tgcagaatag ttaacacctt tgttttatcc
     2221 ttttgtgtct taattatatg atataaaggc gcctggcgtt atcggatagt aatagatgct
     2281 agttatcaac atttcacaat tgaaggaaat aaagttgaag tactcaacaa aaacttactt
     2341 cagaattaaa tttttggggg gaacataggc atcctatgac aggtgaccac aagcccctca
     2401 acgcaatcta atattttaca aagtggtaaa attctttcgt tcttcgtttt aatatacagt
     2461 catttattga ttctattaca ttaatattcc tacgcttcgg ctcacataat taacaggact
     2521 tcgagtccgt taaacttggg atcaactaat ttctacggat tagggataac agggtaatgc
     2581 ggccgc
//
