LOCUS       sequence-192466-        2809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, 
            Tpm2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Skoumpla K, Coulton AT, Lehman W, Geeves MA, Mulvihill DP
  TITLE     Acetylation regulates tropomyosin function in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2007 May 1;120(Pt 9):1635-45.
  PUBMED    17452625
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-05-1"; volume: "120(Pt 9)"; pages: "1635-45"
COMMENT     Sequence Label: pJC20Tpm2
FEATURES             Location/Qualifiers
     source          1..2809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     392..411
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        392..410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             442..464
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1029..1047)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1043..1090
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1135..1153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1221..1325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1326..1394,1395..2186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1544..1563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2357..2809,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc aggatctcga tcccgcgaaa ttaatacgac tcactatagg gagaccacaa
      421 cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatataca tatggagaag
      481 atcaaagaga aattgaatag cttaaaatta gaatctgagt catggcaaga aaaatatgag
      541 gagctgagag aacagctcaa agaattagag caatctaaca ctgaaaaaga aaacgagatt
      601 aaatctttgt ccgctaaaaa cgagcaactc gacagtgaag tagagaagtt agagagtcaa
      661 ttgtctgata caaagcaatt ggctgaagac tccaataact tgagatctaa taatgagaac
      721 tacaccaaga aaaaccagga tctagaacaa cagctagaag acagtgaagc caaactgaag
      781 gaagcaatgg ataaactgaa agaggccgat ttgaactctg agcaaatggg tagaaggatc
      841 gttgctttgg aagaagaaag agatgaatgg gaaaagaagt gcgaagaatt ccaaagcaaa
      901 tatgaagaag ctcaaaaaga attggacgaa attgctaatt cattggaaaa tttatgagga
      961 tcccgggccc tagctaactg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     1021 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1081 gggttttttg ctgaaaggag gaactatatc cggataattc ttgaagacga aagggcctcg
     1141 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1201 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1321 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1381 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1441 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1501 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1561 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1621 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1681 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1741 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1801 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1861 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1921 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1981 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2041 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2101 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2161 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2221 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2281 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcag
//
