LOCUS       sequence-19248-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf171
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             516..557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             567..584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(607..624)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catggaactc gttaatattt ttttagaaac
       61 ggatgctgga agagtaaagt ttgccataaa aaataccgac gatgtatgtg cctcggagtt
      121 aataaataaa tttgtggaac tgttaagtga atacattcac attgaccaat cagaatttta
      181 tttggtggta aaggataagg atatttttta ttttaagtgt gatagggggt ctatttcgat
      241 tgtaaacaat gagttttatg tctttgacga acccttgctg tttgttaaag atttcactaa
      301 tgtaacgggg gttgaattca tagtcacaga aaccatgccg tgtagaatta taccaaaaaa
      361 taatcacgcg gttatttcag tcgtgactaa tcataagttt tataatgggt taagtttata
      421 agcaattccc ggggatccga ataaagggtc aagacaattc tgcagatatc cagcacagtg
      481 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
      541 tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct
      601 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccc
//
