LOCUS       sequence-192480-        7382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R
  TITLE     The E6 and E7 genes of the human papillomavirus type 16 together are
            necessary and sufficient for transformation of primary human 
            keratinocytes.
  JOURNAL   J Virol. 1989 Oct . 63(10):4417-21.
  PUBMED    2476573
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 Oct . 63(10):4417-21."
COMMENT     Sequence Label: pB-actin
FEATURES             Location/Qualifiers
     source          1..7382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3453..3471)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3539..3643
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3644..3712,3713..4504)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3862..3881)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4675..5263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5164..5183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5417..5434
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcgccgctg ggttttatag ggcgccgccg cggccgctcg agccataaaa ggcaactttc
       61 ggaacggcgc acgctgattg gccccgcgcc gctcactcac cggcttcgcc gcacagtgca
      121 gcattttttt accccctctc ccctcctttt gcgaaaaaaa aaaagagcga gagcgagatt
      181 gaggaagagg aggagggaga gttttggcgt tggccgcctt ggggtgctgg gcccgggggc
      241 tgggggcgcg cgccgtggcc cccgcgcccc acgctgggca gtgcccggtt cggccccgca
      301 tggccaggcc tgcccccggc ctgcccgtct ctcgggcccc ccacccaccg cgggacatcc
      361 taggtgtgga catctcttgg gcactgagcg cccaggtggg gtgggccagg gtctgcacgg
      421 gtgccagggc cctgggttct gtacgctcct gcagaaggag ctcttggagg gcatggagtg
      481 gccaggcagt cactccccct tgccgacttc agagcaactg ccctgaaagc agggcctgag
      541 gacctctggc tgtggggctc agctagctaa atgtgctggg tgggtcacta gggagagacc
      601 tgggcttgag aggtagagtg tggtgttggg ggagtcaggt ggcttgcggc cacttagaag
      661 tcgcaggacc acactcccca ggacagggca ggggccagcg gtccagtggc tggaggtggc
      721 ccgtgatgaa ggctacaaac ctacccagcc gcagccctgg gaaggaaggt gggctctaca
      781 gggcagggca ccttttaccc tggagctgcc tgcttttgag ggtaacagtc acgcccagcc
      841 aagaccaggc ctggggcgtt agtgggtgac ctaggcactg cggggcgggg gggctgggtc
      901 tacacagcct gggtctgggc ccaccgtccg ttgtatgtct gctatgcgca gccacagctg
      961 aactgccctc ccagaccatc tggaggccgc tgggggactc tggggaccaa gactccatgt
     1021 gccacagagg attgggggcg gggcggtgct aggaactcaa agccagcctg ggaagaccct
     1081 gtccttgtca ccctttcttg ccttgggtct gtccactgag tagcacacaa gaccgggtgg
     1141 gcagggtccg ttctgctccg ggaatcacag actgtgtgta cccaggtggt gggcatgcag
     1201 cgatcagtgg cgtgggacca cagaggggca ttcggctcag cctagaggaa cctgccttcc
     1261 tcctcctccc cctccttgga ggtggtgaca agaagctgga tttttacaca aaaataggaa
     1321 gagtggtgag gtgggggcac ctcaggcctg cggggctctt tgcagcgcgt agtaggtgtt
     1381 cattgagtgt gtgtcgcaat aggcaaagat gagtccatat aaggacatgg aacctgtggc
     1441 caggaaggag gctgcttgtt ccagttcacg gtacaaggct gtgctcagcc aatgggacct
     1501 gctcctccct tgaaggttgc agaggccaca gcctggtggg aaagatgacc accacccagc
     1561 acacagtggc agacacaggt tcacagtcca gaagacttgc tgagcctcct ccatcaccac
     1621 cccacgcaag ggcaaacttc cccagcctgt atactccctg cctcctgggg atgggactgt
     1681 cctgtccctg gccttgtatc tttcagctgc aggcgtcttg ccagctgcct caggacctgc
     1741 caacgctaca gttcacccgg ctggcgttgt ggggcgatgg gcggggggcc cttcttgctg
     1801 cctgttgatg agggctggtt ctgggggttc gtggaacgtt gaactgggtg gagtgctggg
     1861 ggggtggggt gggcagtggg gatgggaccg gatctacaga gcagggatga gaggttgtca
     1921 ggtgggcggc tccaggcagc aaggtggggt gcagagcaga cctgatcccc aggcaggacc
     1981 tgtgttgccg ctgggcctcc attcagctgg ccaagagaca ggagctccag ccccaccccg
     2041 ccccgggctg cagcattcct ggcctgtcct cctggcttgt ggttccctct gtctgcagcg
     2101 gccacttcct agtgctctcc tatgcaccct tcaagtccca tcccagaaag cccctctttg
     2161 gggaagcgtt ttagcctctg cagccacgcc ttcctcccta cctcagacca aggcttgcag
     2221 ctggcattta gaactgggcc tcagccacca ggctgcgagc cctcccaggg tctccgtcac
     2281 ccccactcct tcccactaca cccaccccag ggctcctcgc tgttgaatga gtgactgcct
     2341 gggtgtcctc aggtggcagg ttggaggtgg ccgagcagcc tggggtgagg ggcagggagg
     2401 agctgaagag agaaagcaag cagcggagca ggaaaagagg aagtagaggt gcctggccag
     2461 ggggcgggcc gagcagggga gaagtgaaca ggttctcttc tgtgcttggg aactttgccc
     2521 tgtaagggag ctttcggagc tgtcactggg gcacgcacgc agcccccgga gaaccctcgg
     2581 ttgaactcag ccaaggggac tccacctgcc tggctgggac ttttgttttt tcccctccaa
     2641 gcctttgctt tggtgacacc cccaccctgc aaggcctcca ggcaaccaac tcttacagcg
     2701 cagacccagg gaccactcct tggccctccc gcaaaacaac cacccagcca ccttgccctg
     2761 ggcactgcag cgccctccct atcctgccag gagggaatcc aacctccttt ttattattat
     2821 tactattttt gatacaaggt cttgctgtga tgcccaggct ggagtgcgat cacagctcac
     2881 tgcagcctcg acctcctggg ctcaaagcct tcacgagtag ctgggactat aggcacacac
     2941 ttccaagcct ggctaatttt taaattatct gtagaggtat ggcttctcac catgttgccc
     3001 aggctggtct ccagctccgg ggggctcaag cgatcctccc acctcagcct cccaaagtcc
     3061 tgggattaca ggcatgagcc accgtgcccg tcctcaagct ccattttgta gaggaaggaa
     3121 ctgagacaga gcttccctcc aagacttgag ttagctctag gaggacaggg accctgaaca
     3181 cggtaggtgc tcagtgagag gagaatgaat gaatgagggg gggcaacatt ccaggagaca
     3241 cacccccatt cacagactag gaaactgagt caggactggt gaagtgctca agtccccaaa
     3301 tgcggtggga aggactctag gctcagggtc cctgaggcgg cagccctgcc tcctgcctca
     3361 cactgcccct ccctggtaaa gggtcccctg gaatgtcccc aaggcctgag tccaaaggct
     3421 gtttgaaagt cggaattcga tgataagaaa gggcctcgtg atacgcctat ttttataggt
     3481 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3661 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3721 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3781 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3841 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     3901 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3961 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4021 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4081 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4141 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     4201 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4261 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4321 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4381 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4441 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4501 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4561 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4801 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5341 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5401 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     5461 ttttctcctt acgcatctgt gcggtatttc acaccgcata gatcctcacc cccttttctt
     5521 ccttcattct ctgctggtgt caatcacagc ttctagcaga gggaaggcag gccaggcttg
     5581 gtttcttctt gcgagagtat ttaactcagg gccctgtaga acaatgagaa tctgacctgc
     5641 aactagctgg gcgtgctggg gcatgcctgt gtagtttcag ctacttggga ggctgaggca
     5701 ggagaattgc ttgagcccaa agttgaggct gcagtgagcc atggttgtgc cattacactc
     5761 cagcctgggc aacacaagac cccgtctcag aaataaaaag agaacctggc ctgcagtgcc
     5821 aggcaggccc tgaggtccag gagcctgggt atctccctct gcagcatggg tcacgaacaa
     5881 actgggccct cagaggccac gggatggcgc ccagtctcca gtcacaaggc agaatccaga
     5941 cctcagccca tagctaacca gagctgtctg caggccagat atggccccat ggacccccca
     6001 ccccaacttg actttgattc caggtccccc tctgtctgga tgaacaggta ggaattgcgg
     6061 cctctcctaa gcctatggga gcctgggcca ggctgaccct caaggctgac agccacgatc
     6121 ccataggtga aggcaaaggc gtggcagcta ggaacactca ctacaccccc actcaaggga
     6181 acaccgtgcc cagctaccct gagacagccc cactcccagg aaatgcaggt gccaaccagc
     6241 cccagtgagg catggcacct gagccagaca cccccaaata gtcccttccc acctcctcaa
     6301 gcatctgcac tctgggtgaa cccagaaaaa ctggggtacc tggggagaca tagaagggcc
     6361 aggccaggaa ctccccaata agcaggaaca gagacctgac ccctgagcct cccccaccct
     6421 ctaaggctgc tcaatgtcaa ggcaggaggg atctccacac acctgcactc tgggtgagga
     6481 caagttggcc cccactgccc cacccttttg cagggttcac cctcctgcag ggttcaccct
     6541 ctgctgcccc caacccagcc acaccacaaa gtcacacttg gcctcatttt taaggtgtgc
     6601 acttttattc aactggtctc aagtcagtgt acaggtaagc cctggctgcc tccacccact
     6661 cccagggaga ccaaaagcct tcatacatct caagttgggg gacaaaaaag ggggaagggg
     6721 gggcacgaag gctcatcatt caaaataaaa caaaataaaa aagtattaag gcgaagatta
     6781 aaaaaatttt gcattacata atttacacga aagcaatgct atcacctccc ctgtgtggac
     6841 ttgggagagg actgggccat tctccttaga gagaagtggg gtggctttta ggatggcaag
     6901 ggacttcctg taacaatgca tctcatattt ggaatgacta ttaaaaaaac aacaatgtgc
     6961 aatcaaagtc ctcggccaca ttgtgaactt tgggggatgc tcgctccaac cgactgctgt
     7021 caccttcacc gttccagttt ttaaatcctg agtagcttat cgatgataag ctgtcaaaca
     7081 tgagaattcc aagcttgggc tgcaggtcga ctgatcctgc gagaatagcc gggcgcgctg
     7141 tgagccgaag tcgcccccgc cctggccact tccggcgcgc cgagtcctta ggccgccagg
     7201 gggcgccggc gcgcgcccag attggggaca aaggaagccg ggccggccgc gttattacca
     7261 taaaaggcaa acactggtcg gaggcgtccc cgcggcgcgc ggcaggaagc caggccccaa
     7321 ccccctccca accgggcgcc agccccgcct ccgcccggtt caaacagcgc cgggtcggcg
     7381 cg
//
