LOCUS       sequence-192494-        2744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mTagRFPT-linker sequence for N-terminus tagging of
            human CETN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-22:CETN2-mTagRFP-T
FEATURES             Location/Qualifiers
     source          1..2744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1001..1729
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 agtgtacacg tcggttgcct aacaaccggc agcggactcc tttggctatg gtgagtgctg
       61 ccgctctgtc gcgccccacg cccagcggct gagcccttcc caggccccag ctcggccacg
      121 ccgcctctcc tgcgcctttc tcggctttag cgcaccagtc tttcctttga gctctttccc
      181 gggagagtag ggtacagacg ctaaccaacc tactgggcct gcgcttcgct gctgcctcct
      241 cttcacccac aacaggccca cacccgcccc tcgggctccc cttgcattcc ctgatcccct
      301 cggtgaacct ccctgggcaa aaaagactcg aatccctcac aggcccctga ctctctcaga
      361 ccttggcccc ctggcctcct cattctgcca aaatttccac acacagctgg gaaaaggtgt
      421 atcgtgttct gttacaatgt tttttacaat ttaaatacag gggcattcag gggatgcttt
      481 agcttcttgg agaaggcacg aaggtgccag atggattgag ttttattact cggtactgac
      541 acaatataca tcttaacaac ttactaatat ttggccaaat ctagaagtaa ttgggaagtc
      601 ggagtactta ctgggaaggg gagtactggg agtgggcatc gtaagactgt tttcagaacc
      661 aggatcccca cgttcgggaa gagctgatta tctctagtta ttcacagtca tcttcttgac
      721 tggctctctt ttgggatgat tttcagtaag aggtgtcctt actgaacaag tgatctccct
      781 gcttctagaa acaacagtaa agaaaaggga ggggtagaag ttcatggaaa gattcattgt
      841 tcttgccagt aaaccctgtt atctttcttt ttaagcaaag ctttggaatt ggttatataa
      901 atgactttaa aaatacatat gggtgtctgt aactatgtta tgtaaatatg gactgtatga
      961 tatattattg tgcttgtgta ttgcataaca tataaaggcc gtgtctaagg gcgaagagct
     1021 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     1081 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1141 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1201 cggcagcaga accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt
     1261 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1321 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1381 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     1441 cgagatgctg taccccgctg acggcggcct ggaaggcaga accgacatgg ccctgaagct
     1501 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     1561 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     1621 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     1681 ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaaga gcggcctgag
     1741 aagctccaat ttcaaaaagg caaacatggc atcaagttct cagcgaaaaa gaatgagtcc
     1801 taagcctgag cttactgaag agcaaaagca ggagatccgg gaagcttttg atcttttcga
     1861 tgcggatgga actggcacca tagatgttaa agaactgaag gcaagctctg tgcattcctg
     1921 ccgtactgcc cttcctttcc tctgcctctt ccttgtcatc tccctatcac ctctctaccc
     1981 cttttgccct cttctccctg cagtaagtaa caggggaggt tgtactgtgc tgttttcctg
     2041 tctttactta ctttgagtat tattcaataa aattgattgc aaatatcaat tagatcaatg
     2101 tcatagtgtt ccccaatcat attaacatga ccacatcaga tgctggctta atgctctcaa
     2161 cactgttatt tttgctcctg ctactttaga tgtgcttatt gagacgatcc tgttttcttt
     2221 ataggtggca atgagggccc tgggctttga acccaagaaa gaagaaatta agaaaatgat
     2281 aagtgaaatt gataaggaag ggacaggaaa aatgaacttt ggtgactttt taactgtgat
     2341 gacccagaaa atggtaagta gctaagacaa ttatcatggt atggagcccc agtcatctgc
     2401 agaggtccat ggcgtcttcc tgttaaaaca gtcatgtgac cacagagcag tccaggtagc
     2461 aactgcatca ggaggcctgc cctcccatca tgttactttg ggctcccaaa acaagccctt
     2521 gtgcttcttt ctcaagactt ccaagcagat acctcttccc acagaattcc aaatcagtta
     2581 ggaaatagat atgggatagg gagagatata gcgataggta gcaaacgtca ccttggatgg
     2641 agtgcctggc ttgcctggtg tttctttgag gccaaaatgg gcttcctgac acttgccgca
     2701 agtctagact cgagttacat tgttaaaaag gaaagggtga cctg
//
