LOCUS       sequence-192495-        2777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human LAMP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-19:LAMP1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1064..1066,1067..1780)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1106..1127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1367..1386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1714..1735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggtttttcc tacaaggaat ccagttgaat acaattcttc ctgacgccag aggtgagaac
       61 ccacaatctc tgcgagcccc gcccccgccc ccgcccgcgc ccccagggta ttctggagcc
      121 actagacctc tgtgtgtgtt gcagaccctg cctttaaagc tgccaacggc tccctgcgag
      181 cgctgcaggc cacagtcggc aattcctaca agtgcaacgc ggaggagcac gtccgtgtca
      241 cgaaggcgtt ttcagtcaat atattcaaag tgtgggtcca ggctttcaag gtggaaggtg
      301 gccagtttgg ctctggtgag tgtcaccgag ggcagctgtc gcggggtgtg gaggacgtgc
      361 ttcagactcc gcctgtggac gtttagtcgc ttccgtgtgg gctggggcga cgcccctgtt
      421 cctctgcaag gagctgtttc ttcttgccgg tctgagattc tagaggtaac tccccctgct
      481 ttagagaggc ccagcgtgtc tctcagctgg gagcccctgg taccatttga gagtaaggga
      541 atcattttaa gaaacagtgg tggcgcttct cccatgaacg ttgaatacaa cactgtcatc
      601 tgatgtgcac agaggccctg gacgcagcac agctgtccgg ccacagcccc tgattccaga
      661 cgggggagag acgtgtgtgg tttcctcgcc gtgcagagag caccagtctc tgcagcggct
      721 tccccaagtg acaattccag ctagagctgg aaccttccgc cagggttcct ctttgccttt
      781 tggctgatgt gggggagtgg tggggagaga ggcctgtttg caggcccctg tgtgagcaga
      841 gccctgacac catccgtctg tcttggcagt ggaggagtgt ctgctggacg agaacagcat
      901 gctgatcccc atcgctgtgg gtggtgccct ggcggggctg gtcctcatcg tcctcatcgc
      961 ctacctcgtc ggcaggaaga ggagtcacgc aggctatcag actatcgaat ttggcagcac
     1021 cggcagcacc ggcagcaccg gcgcggatcc gccggtggcg accgtgagca agggcgagga
     1081 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1141 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1201 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1261 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1321 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1381 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1441 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1501 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1561 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1621 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1681 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1741 cgccgggatc actctcggca tggacgagct gtacaagtag cctggtgcac gcagccacag
     1801 cagctgcagg ggcctctgtt cctttctctg ggcttagggt cctgtcgaag gggaggcaca
     1861 ctttctggca aacgtttctc aaatctgctt catccaatgt gaagttcatc ttgcagcatt
     1921 tactatgcac aacagagtaa ctatcgaaat gacggtgtta attttgctaa ctgggttaaa
     1981 tattttgcta actggttaaa cattaatatt taccaaagta ggattttgag ggtgggggtg
     2041 ctctctctga gggggtgggg gtgccgctgt ctctgagggg tgggggtgcc gctgtctctg
     2101 aggggtgggg gtgccgctct ctctgagggg gtgggggtgc cgctttctct gagggggtgg
     2161 gggtgccgct ctctctgagg gggtgggggt gctgctctct ccgaggggtg gaatgccgct
     2221 gtctctgagg ggtgggggtg ccgctctaaa ttggctccat atcatttgag tttagggttc
     2281 tggtgtttgg tttcttcatt ctttactgca ctcagattta agccttacaa agggaaagcc
     2341 tctggccgtc acacgtagga cgcatgaagg tcactcgtgg tgaggctgac atgctcacac
     2401 attacaacag tagagaggga acatcctaag acagaggaac tccagagatg agtgtctgga
     2461 gcgcttcagt tcagctttaa aggccaggac gggccacacg tggctggcgg cctcgttcca
     2521 gtggcggcac gtccttgggc gtctctaatg tctgcagctc aagggctggc acttttttaa
     2581 atataaaaat gggtgttatt tttattttta tttgtaaagt gatttttggt cttctgttga
     2641 cattcggggt gatcctgttc tgcgctgtgt acaatgtgag atcggtgcgt tctcctgatg
     2701 ttttgccgtg gcttggggat tgtacacggg accagctcac gtaatgcatt gcctgtaaca
     2761 atgtaataaa aagcctc
//
