LOCUS       sequence-192496-        2729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human MAP1LC3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-25:MAP1LC3B-mEGFP
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatgactgcc ctttcacaga tgagtctgag actcaaaaac tgtcactagc gtaaggttgt
       61 ccagcgagtt tagtggctgg gaggctgagg ggtagacagg gcggctagcg atgtggggca
      121 ggcctggcgg tcgccacaga cgacctaacg gtaggaaaat cttacagcca ccaggagagt
      181 tccaggcgcc gcggcagggg gactgggaga ggggactgcg cccagaatga aggctcggga
      241 caaaagcagt tgcgcaaacg cgccaaggct gggcgtcgag tgaccgcggg cggaggtcac
      301 cagcggccgc tccccggaag ccacccacgg accacgcgcg cccctgcacg cagagggggc
      361 cagggctcca cgggcgagcg gcgaccctgc ctccgggaga cggcgcggcc tgccctgcgc
      421 gcctcagccc cgggtgccgg cgtctcgggc agcaccacca agtctctctg gaggggaaag
      481 gatggtcgga tttgccccat gtcccttcct ctgacccctc cctcaagagt gccccgggac
      541 accccgcctg tggctcagcc tcccccgccc cgcgctgcca tctcctcagg gccgggcagc
      601 aggctcccga gcgcccacag acccggggtg cggcccagcc cacaaccgtc acctcagggg
      661 cctcaggcgc ccagcggtgc tgggcggggc tggggcacga ccgggagcat gcgcagagcg
      721 cgcgtttcgc ccatcgcgca cgcgcacaca cctgctccgc ccccacgctg cgtgccgctg
      781 ctgggttccg ccacgcccgt catggcggcg gccccggccg gctctggccc cgcccctcgg
      841 tgacgcgtcg cgagtcacct gaccaggctg cgggctgagg agatacaagg gaagtggcta
      901 tcgccagagt cggattcgcc gccgcagcag ccgccgcccc cgggagccgc cgggaccctc
      961 gcgtcgtcgc cgccgtagcc gcccagatcc ccgcaccatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagagcggc ctgagaagcc cgtcggagaa
     1741 gaccttcaag cagcggcgca ccttcggtga gtgtcgccgc gagggcggcg ggtgcggcgg
     1801 ggccggggtc cgagctgtgg agggcggcag ggcctgggac gccgtgaggg gtcggggccg
     1861 agccgggagg ccgagcgggg ccggtggctg ccggccggcg gggccgaggg atgcggggcc
     1921 cggggccccg tgagggaccc aggccggggc cgagccggga gggccagggg ctggtctggg
     1981 ccggggcggc ctgcggaccc ctcggaggcc tgggaggagg cagccggcgg agggcgggcg
     2041 ggggccggtg cgctcggggc cccgggcctg ctgaatcacc ccgcgccctc cgcggcgtgg
     2101 tgcctgccgt accccggcca ccgccgcccc cgcaagcgcg ctgcgggcga gggtcgcgct
     2161 tctggggccc aacagccccc ggggccgcgc tgggccgttc ggctctgaag cggggctgcg
     2221 ccgggaccca gcgcgtcacc ttcagggctg gttttcccgt cgagggagcc cgcccgggtg
     2281 cgagtgcctc ttacagacct cagtgcctcg gtcgagagga ggggaatgtg ctgggccccg
     2341 gcctcgccgc gcccgggaca gcccacagcg gtgttgccag ttcctgtctg tagcccagga
     2401 gctggggtgg gagacttctc ttgggtttgc gattacgttc tcaccttggc accaggaata
     2461 ccgaatacat cccttttctg ccggttcaga ctggaaaccc agcttaggtg tgctggcatt
     2521 tggttaaacg tgcaggtgtc accatcacaa tatttactcc acgcagatga tgcgaagtga
     2581 ttatttcccg cttgagcaag agaacgccgc agatccaggt atctgttgtt gcctaagttc
     2641 cagacaccgg tttggaaggg aagcaccgtg ttcatcgcgg tggactgcga atgcctgccg
     2701 ccatcctcgt caggagccca cacagtggt
//
