LOCUS       sequence-192498-        2746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mTagRFPT-linker sequence for N-terminus (second 
            exon) tagging of human TUBA1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-28:TUBA1B-mTagRFP-T
FEATURES             Location/Qualifiers
     source          1..2746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1001..1729
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 aaagcaataa gaggactgcg gaagagctcc ctgtcaatgt accgctctac accagtgtat
       61 tacgacagtt cgtacacaac agtctgtaga ggccacctgt ctctccctgc tgcgttagga
      121 attcagggga gcaggtggtg gcagtaaggg attttgaggg aacggaaatc ggatcttgac
      181 ccagatctgg gccgccgata atctcctact gcgctcagac tgctgtggag gtgttaggct
      241 gagcccgatg ccggcaggca agggaggatg ggcggcttgg gcagcgcctt tgcagacgtg
      301 gccatttcgt gcctctgcag caccgccggg gggcgcaaga gcgcgcgccc ggaattgctc
      361 attcatcctg tgccgcagag ccccgcccct tgtccctgcg gacagacatt tcttctgcgc
      421 tggtctggcc acgtgcttcc tgtgctagga gctgcccgga aatgtgacca cctagtctaa
      481 agtgggcttc tggggcctga gcgctggatg gatgcccacc ttcctgtctt ggtcctccaa
      541 aggaggaagc tgtgactgag ctgtcttggt ctggaaggag gccttcccgg tttaggatgg
      601 gaaggtaaca ttcattaaaa gcaacgtaga ctatagtgta gctgttctca aaagtagtac
      661 atcttagaaa aggatcttta gaaaagatcg ctttagaaaa ggaaattcgt tttcagatta
      721 cgtgagtagc ctaggtaaca cagccagacc tcatctccac aaaaaaaatg aaaaaattag
      781 ccagcttggt ggtctgtgcc tgtggtccca gctgctccag aggctgaggt ggggggatga
      841 ctggagccta ggctgcagtg agcctagatg gcatcactgc actcaagact gggcgacaga
      901 ccttatctct aaaaaaataa agattgcatg agtattttgt tccacttgac agtcatcaat
      961 agattggttt aaattgtgat atctttttta cttaccgcag gtgtctaagg gcgaagagct
     1021 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     1081 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1141 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1201 cggcagcaga accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt
     1261 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1321 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1381 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     1441 cgagatgctg taccccgctg acggcggcct ggaaggcaga accgacatgg ccctgaagct
     1501 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     1561 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     1621 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     1681 ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaagg gaggttcagg
     1741 aggcagcgag tgcatctcca tccacgttgg ccaggctggt gtccagattg gcaatgcctg
     1801 ctgggagctc tactgcctgg aacacggcat ccagcccgat ggccagatgc caagtgacaa
     1861 gaccattggg ggaggagatg actccttcaa caccttcttc agtgagacgg gcgctggcaa
     1921 gcacgtgccc cgggctgtgt ttgtagactt ggaacccaca gtcattggtg agttgacctc
     1981 agtaacctga gatcccagga tgctgggaca ggaggtctgt ccaggggctt ctcttgtcac
     2041 tcactcactc cctccgtcct tctctccctc ctccagatga agttcgcact ggcacctacc
     2101 gccagctctt ccaccctgag cagctcatca caggcaagga agatgctgcc aataactatg
     2161 cccgagggca ctacaccatt ggcaaggaga tcattgacct tgtgttggac cgaattcgca
     2221 agctggtaag caccacatat aaatatgcat ttaatgtggt gtgatagttc cagtgcaagt
     2281 tgggtggagt gactgacatc attcattctt tggcacctac caaaatgtgg aataggctgc
     2341 ttgctatatt aattggactt ctaaatcaga tagtccctag gttatggaca gtttgtggat
     2401 atgtctgttt tgccaattcc ttgtgcttac atcagtgaga tatggttcgt aatctaaaaa
     2461 gttgaaatag aaattctaag ataatgtgtc ctggcattaa aatattacat ttttttattc
     2521 ccctacaggc tgaccagtgc accggtcttc agggcttctt ggttttccac agctttggtg
     2581 ggggaactgg ttctgggttc acctccctgc tcatggaacg tctctcagtt gattatggca
     2641 agaagtccaa gctggagttc tccatttacc cagcacccca ggtttccaca gctgtagttg
     2701 agccctacaa ctccatcctc accacccaca ccaccctgga gcactc
//
