LOCUS       sequence-192499-        2759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human ST6GAL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-26:ST6GAL1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1046..1048,1049..1762)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1088..1109)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1349..1368)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1696..1717
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacaatttaa gggcgtaccc cttaaaaact cagtcatcaa gataaataat atttcactac
       61 agtatcttca gaaaacacaa ttaatgtaaa aattatgatg agtgaaatat caagtattta
      121 aataaagata ggatcagtaa cagtgctgtg cagagtttat tggaacaatg ctggccaggg
      181 gcccccttct tggattttct ctacatctca tcattcagac tgtggagtgc tcctgttcca
      241 gctgtccctt tgcccagcta agggaagaaa agtcttccct ccccaacact gtttcatgca
      301 aatgctataa ggaaaaatct gctcccaaat agcaccatcc aagaggttct gcaggatgtc
      361 acagtgctag aaagtgctaa ctacacatga tgccactgtc tcctcaatca gctcagtaat
      421 cttgtcctct ttctccaaat aaattattcc atggatacat tcattggctg attgatggag
      481 tcatttaatc tactagcatt tagggaggat ctattattca ccaggccctg gcctaggtgc
      541 tggggattca aaggtgtata accctcacac agccctgcat cctaaggaac actgctgggc
      601 ttggcttgct gaaacttaga ttgggaatca cagtcataaa tagaaactcc agagggaaag
      661 ctctccaaat ttggggtcat gagctgctga acccactggg cagagctctg gggtgctggg
      721 gtgggttgtc aggcatgact cacctctgct cccctctcca ggtatcatca tcatgatgac
      781 gctgtgtgac caggtggata tttatgagtt cctcccatcc aagcgcaaga ctgacgtgtg
      841 ctactactac cagaagttct tcgatagtgc ctgcacgatg ggtgcctacc acccgctgct
      901 ctatgagaag aatttggtga agcatctcaa ccagggcaca gatgaggaca tctacctgct
      961 tggaaaagcc acactgcctg gctttcgcac aattcactgc ctccagagca ccgtgccacg
     1021 ggctcgggac ccacctgtgg ccaccgtgag caagggcgag gagctgttca ccggggtggt
     1081 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1141 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1201 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1261 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1321 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1381 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1441 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1501 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1561 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1621 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     1681 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1741 catggacgag ctgtacaagt aagcacaccc tcctcactct tctccatcag gcattaaatg
     1801 aatggtctct tggccacccc agcctgggaa gaacattttc ctgaacaatt ccagcctgct
     1861 ccttttactc taggggcctc tgtcagcaag accatgggga cttcaagagc ctgtggtcag
     1921 gaaatcaggt ccagccttcc ctgtagccag acagtttatg agcccagagc ctcctgccac
     1981 acacatgcac acatatctag cattctttcc agacagcatc ctccccgcct tccaccttgg
     2041 tagatgcaag gtctatctct cccatcaggg ctgccaaagc tgggctttgt ttttcccagc
     2101 agaatgatgc cattctcaca aaccaatgct ctatattgct tgaagtctgc atctaaatat
     2161 tgatttcacg ttttaaagaa attctcttaa attacaattg tgcccaatgc agggtggctc
     2221 tggggggcaa gtaggtggta caggggattg gaaacatgct ccgcgcctcc agagaaaagt
     2281 tgctcccgag gtccatgccc ctggaacgtg ttcctatcac tctggctggt tgggctggtc
     2341 cttagactgg gtgcttatga ttaaagggtc ttggttagcc cactttccct ctccatgtgg
     2401 agatggaagg tagagaagga tacagtgtct atcctcaagt tgctacggtt cagtgagaga
     2461 ggcagacatc tgaacaggca ggtaggattc agtgtgctca gtgcactggg gatttggaga
     2521 gagatgggct tgctctctct gtgcacccag gagggccacg cacttaaaac tgtgtttgtg
     2581 gatcagagaa ggctttatag cacagggggc attcagatga gtcttagagg aagagaagaa
     2641 acatggcaag cagattacat ctgagccgtt tgaattgtgt ttttctttct tcccatgttt
     2701 attttctaag atctacctga acttagagac tcaagatatt tttttaggaa acctcctac
//
