LOCUS       sequence-19252-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf175
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             349..390
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             400..417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(440..457)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    446..557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cagtaccctt taccgaattc cagctgacca ccatggataa gttgtacgcc gctatatttg
       61 gtgtatttat ggggtctccg gaagatgatt tgacagactt tatagaaatt gttaaatctg
      121 ttctaagtga tgagaaaaca gtcacatcaa ctaataatac cggttgttgg ggttggtatt
      181 ggttaattat tatttttttt atagttctta ttctactact attgatatat ttgtatttaa
      241 aagttgtttg gtgagcaatt cccggggatc cgaataaagg gtcaagacaa ttctgcagat
      301 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
      361 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
      421 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      481 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      541 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg ggggtggggt
      601 ggggcaggac agcaaggggg
//
