LOCUS       sequence-192522-        8372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid used to target the pre-modified Rosa26 allele with Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: px330 Rosa26 AscI LH291Gu1 (LH500)
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      238..247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             247..312
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             319..339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             364..4464
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             364..4464
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4465..4512
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4540..4557)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4546..4753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4762..4902
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4762..4891
                     /label=AAV2 ITR
     rep_origin      4977..5432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5064..5083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5274..5295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5449..5468)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5568..5590
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5628..5646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5714..5818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5819..5887,5888..6679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6037..6056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6850..7438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7339..7358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7500..7748
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7500..7520
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7671..7690
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7770..7845
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        7942..8227
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
       61 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      121 gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt
      181 aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc
      241 accatggact ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac
      301 gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
      361 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      421 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      481 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      541 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      601 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      661 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
      721 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
      781 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
      841 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
      901 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
      961 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1021 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     1081 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1141 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1201 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1261 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1321 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1381 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1441 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     1501 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     1561 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     1621 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     1681 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     1741 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     1801 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     1861 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     1921 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     1981 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2041 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     2101 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     2161 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     2221 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     2281 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     2341 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     2401 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     2461 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     2521 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     2581 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     2641 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     2701 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     2761 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     2821 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     2881 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     2941 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3001 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3061 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     3121 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     3181 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     3241 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     3301 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     3361 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     3421 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     3481 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     3541 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     3601 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     3661 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     3721 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     3781 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     3841 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     3901 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     3961 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4021 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4081 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     4141 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     4201 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     4261 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     4321 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     4381 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     4441 gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca
     4501 aaaaagaaaa agtaagaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt
     4561 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4621 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4681 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     4741 ggcatgctgg ggagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4801 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4861 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4921 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4981 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5041 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5101 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5161 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     5221 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5281 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     5341 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5401 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     5461 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5521 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5581 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5641 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5701 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5761 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5821 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5881 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5941 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6001 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6061 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6121 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6181 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6241 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6301 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6361 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6421 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6481 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggaagccg
     6541 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6601 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6661 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6721 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6961 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg
     7501 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     7561 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     7621 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     7681 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     7741 gaaacaccgc cgcggcgcgc cgaatgatag ttttagagct agaaatagca agttaaaata
     7801 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
     7861 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
     7921 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc
     7981 gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg
     8041 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8101 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca
     8161 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8221 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     8281 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     8341 gggggggggg cgcgcgccag gcggggcggg gc
//
