LOCUS       sequence-192524-        9224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing parBp(mut)-m(sf)gfp fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   van Raaphorst R, Kjos M, Veening JW
  TITLE     Chromosome segregation drives division site selection in 
            Streptococcus pneumoniae.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114.
  PUBMED    28674002
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114."
COMMENT     Sequence Label: pMK17-02
FEATURES             Location/Qualifiers
     source          1..9224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3854..3876)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3962..4102
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4017..4036
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4117..4134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4288..4876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4368..4387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5047..5838,5839..5907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5670..5689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5908..6012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6080..6098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgtctgaatt gtaattcctc tctgacgttc taaaagcgta ttatccgtcc tcgttgtacc
       61 tttgtccacg cttcctaatt ctgtaatcgc tccactgtta tataataagc tttctgttaa
      121 ggtagttttt cctgcatcaa catgagctaa aactccaata ttaataattt tcatgtgatt
      181 ttcctccatt caaaagccca aaagggcata aaaatcccag tgataaatac tcttatcact
      241 gggattttta tgcataacca taggcataca aagcatacag atattctccg gatactttag
      301 aatcacatga taaaggtatt cttaaactgg ggacaaaaaa ctaagccctc ctaaaaaagg
      361 acgtccaatt atttgttccc gctatcaaat tgacagttta tttaagaata ccttgccgca
      421 tatttattaa ctccttttaa atagatactt aaattatagc acgtaagagc atatttgtaa
      481 aggaatctcc aattttttat caaaaagagt acatgattac aaagtatctg taatcatgta
      541 ccaatattta ttattttaca atcttccaat tatcttgttt ttctaatatc aactcaaatt
      601 gcgagatttg ggttgccttt gtttcttgat ctaaatattt cacagcgaca ttcacgatta
      661 cttggctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt
      721 ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact
      781 acgcgatcat ggcgaccaca cccgtcctgt ggatctatcg atgcatgcag ccgacggtac
      841 cgcatgctgc tgatacggcg gtcaatgtta gtcatatgga cacttaaggc aaattgttca
      901 gaactgaata aagctgacgt tttgcttcta tcctttcttt gagttttagt ggataatgat
      961 aatgaacaag gtgttcataa atctattata acaaaggaat gagaaatatg aaggcaaaat
     1021 atgctgtttg ggtggctttt ttcttaaatt tgacttatgc cattgttgag tttattgcag
     1081 gtggagtatt tggttctagc gctgttcttg ctgactctgt gcatgacttg ggagatgcga
     1141 ttgcaattgg aatatcagct tttctagaac tagtagatct cgcggccgcg gatccgaatt
     1201 cggatctcga gtactgatta actaataagg aggacaaaca tgagtaatag tttcggattt
     1261 acagacctta tgaacaagga cgaacacaag cgaaaaaaaa caaatacaaa aaatattcct
     1321 attgaggaga taaaggaaaa tgagaataac aactatgatt tagttgacat tgataagtta
     1381 gctgatagca tagacgagtt aggcttatta caacccgttc ttgttaaaca acgtgataaa
     1441 tatagctatg aattgattgc agggcataga cgatttaatg ctattaaaaa actaattagt
     1501 gaaaatagac tccctgaaga ttacgaagtt ctcgctaaga aagttgatga agacgaggac
     1561 gagcttgtaa caagactaaa gttgcatgaa actaacctac aaactcgctc gctcttaaaa
     1621 atgccagaag aagaaaaaat agccatcatt gatgactata tggatatttt agataaagcc
     1681 aaaaaacaag gggtacaaat caacggaaaa cctgttaaag gaaagactag ggacttaatt
     1741 gccgaaagat ttggtatcag tcattatacc gctcaaaaac tgattagaaa agctaaagaa
     1801 caaggtggtg aagaagaggg agcaaagttc tctccacaaa agaaaaccgc taaaaagcca
     1861 atcacgcagt taaagaaaat tgaaactcaa ttagaaaaat tagagtttga gggaacggaa
     1921 gaagaacaag aaatcaagaa aaaattgatt gagttgttga tgaaatctag ttctagagga
     1981 tctggtggag aagctgcagc taaagctgga tcaaaaggag aagagctgtt cacaggtgtt
     2041 gtgccgattc tcgttgagct tgacggagat gtaaacggac acaaattctc tgttcgcggt
     2101 gaaggtgaag gagatgcaac aaacggcaag ctgacattga agtttatttg cacaactgga
     2161 aagctgccgg ttccttggcc gacacttgta acgacgctga cttacggcgt tcaatgcttc
     2221 tctcgttatc cagaccacat gaaacgccat gatttcttca aatctgcaat gcctgaaggc
     2281 tacgttcaag agcgtacgat cagcttcaaa gatgacggaa cgtacaaaac aagagcagaa
     2341 gtgaagtttg aaggtgacac acttgtgaac cgcattgaat tgaaaggcat tgatttcaaa
     2401 gaagatggaa acatccttgg acacaaactt gaatacaact tcaacagcca caacgtatac
     2461 atcactgctg acaaacaaaa aaacggcatc aaagcaaact tcaaaatccg tcataacgta
     2521 gaggacggtt ctgttcagct tgctgatcat tatcagcaaa atacaccgat cggtgacggc
     2581 ccggttcttc ttcctgataa ccattattta tcaactcaaa gcaaattatc aaaagaccca
     2641 aatgaaaagc gtgaccacat ggtgctgctt gaatttgtga cagctgctgg tatcactcac
     2701 ggcatggatg agctttataa gtaagctagt caaggtcggc aattctgcag tactaggacg
     2761 ccgccaagcc agcttaaacc cagctcaatg agctgggttt tttgtttgtt aaaaatgaag
     2821 aagaaactgt gaagcgtatt tatagcaaag cactcaaaag tttaccttat gggtgctttt
     2881 ttcgtgcttt tttgaaaaga caaaaaaaag aaccttgcca agcaagattc ttttgatagc
     2941 gctatcgctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
     3001 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttctg
     3061 gtgatgaaat caacgtaaca tttaaagctg tcaaagccaa agtcatgaga tggcgtatgg
     3121 agcgtaaagc tgacaagagc ggtgttgcga tgattgagat gaccttcctt gcaccaagtg
     3181 aattgcctca agaaagcact caatcaaaga ttcttgtaga tggaaaagaa cttgctgatt
     3241 tcgctgaaaa tcgtcaagac tatcaaatta cctataaagg tcaacggcca aaagtctcag
     3301 ttgaagaaaa caatcaagta gcttcaactg tggtagatag tggagaagat agccttccag
     3361 tacttgttcg cctcgtttca gaaagtggaa aacaagtcaa ggaataccgt atccagttga
     3421 ctaaggaaaa accagtttct gctgtacaag aagatcttcc aaaactcgaa tttgttgaaa
     3481 aagatttggc ctacaagaca gttgagaaaa aagattcaac actgtatcta ggtgaaactc
     3541 gtgtagaaca agaaggaaaa gttggaaaag aacgtatctt tacagtgatt aatcctgatg
     3601 gaagtaagga agaaaaactc cgtgaagtgg tagaagttcc gacagaccgc atcgtcttgg
     3661 ttggaaccaa accagtagct caagaagcta aaaaaccaca agtgtcagaa aaagcagata
     3721 caaaaccaat tgattcaagt gaagctgatc aaactaataa agcccagtta ccaaatacag
     3781 gtagtgcggc aagccaagca gcagtagcag caggtttagc ctgcctcgcg cgtttcggtg
     3841 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3901 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3961 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     4021 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4081 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5341 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5641 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     5701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6001 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6061 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattaat
     6121 tccttcttaa cgccccaagt tcatcaccaa tgacatcaac tcacatgaac tacatgatga
     6181 acccagttat catggttttg gataagattt ttgaaaaatt cttcccaggc cttgataaat
     6241 atgactttga tgctgctaaa ttgaacaaga aaatcggttt ctggggatct aaattcttca
     6301 tcggtttcat ccttggtatc gttatcggta ttatgggaac tccacatcca attgcaggtg
     6361 ttgcagatgc agataaatgg cgtcttgtta tcaaaggatg gttgtctctt ggtttgactg
     6421 ccggtgtatc tttggaactc ttctcactta tcggttcatg gttcatcgca gccgtagaac
     6481 cactatcaca aggtattaca aacgttgcta ctaaacgtct tcaaggacgt aaattcaata
     6541 tcggtcttga ctggccattc atcgctggtc gtgctgaaat ctgggcttgt gccaacgtac
     6601 ttgcaccaat catgttgatt gaagcagtgc ttctttcaaa agttggaaat ggtatcttgc
     6661 cacttgcagg tatcatcgct atgggtgtta ctccagctct cttggttgta actcgtggta
     6721 aattgctccg tatgattatc ttcggaacac tcttgttgcc actcttcctt ctttcaggta
     6781 cacttattgc accatttgca acagaacttg ctaaaggtgt aggtgccttc ccagaaggtg
     6841 tgagccaaac tcaattgatt actcactcta ctcttgaagg accaatcgaa aaacttcttg
     6901 gttggacaat tggtaacact acaactggtg atatcaaagc aatccttggt gcagtagcct
     6961 tccttgtatt ctatatcggt atctttgctt ggtacagaaa acaaatgatc aaacgtaacg
     7021 aagagtacgc agcaaaagca aaataatgcg ctccgctagc tttacagaca aagaactatc
     7081 cttaatggaa gcactagcaa aaggtatcaa tgaagcaaga aatattgaag gctagtcagt
     7141 aaaattcaga taaacaaaaa gagccgataa gatgagagtt tcatttctca aattatcggc
     7201 tctgcgtctt ggcgtctggc tctttgattg ttattatatt cattttttga acattaatta
     7261 aagtttcgtt cttacatttg gggcagtata aagggaaatt tttaagtata gtatccactc
     7321 gtattcgtag tcttgtttta tttccacaaa taggacacaa tatccacttg tagtttataa
     7381 taacaatctc ctcctttcca ctttaattca aatctatatt aaagaatatt tcatcttatt
     7441 taataagaaa ccatatttat ataacaacat aaaacgcact aagttatttt attgaacata
     7501 tatctcactt tatctatctg actatttaga cgacgggtct ggcaaacagg ttcgccagtg
     7561 gtaacctgat atccttttag ctctgctaaa caaacactaa gcccatttgt aaaaaaagtt
     7621 aaatcattgc gataatcttg aatacatcga gcaggaattt ctccaataat aatgacctca
     7681 ttatttttca gttgagtatt tacgatattt gcacaatatt tgggagcatc gttatatgcc
     7741 cgtgaaagat attcctgtgg tgcataaact ttaaaactaa gatatggctc taacaattct
     7801 gttccagctt ttctaaaggc ttgctccaat acaataggag caagcatccg aaaatctgct
     7861 ggggtactaa cagggctata gtataagcca tacttaaaac agattttaca gtccgtcaca
     7921 ttccaaccat acaatccttg ttcacaacca tagcgtatcc cttccataac tgcattttga
     7981 aatgattgat ttaagtatcc aagagaaacc gagctctcat actgcattcc acttcccaac
     8041 ggaagcggtg atacagataa accaatggaa gcccagaaag gatttggcgg cacttcgatg
     8101 tgaatggtat attctgcatt ttttaacggt ctctccatat aaatgactgt aggctctttt
     8161 agttctatct ccacatgata cttttcttgc aacagtgcac taatcacttc catttgtact
     8221 ttccctaaga aagaaagtat aatttcatgt gtcgtagaat ccacgtaata tcgtagaagc
     8281 ggatcactat ctgagatttc caaaagggca tcaagcaaca tttctctctg ttcaggttta
     8341 ctcggttcaa cagttgtttg tagtagaggg tgcggatttt caatcttttt tctctgtggc
     8401 aatagttttg tatctccaag aacactattt aacttcaaaa gctcattttg caaaataaca
     8461 atttctccag aataagctcg atcaatctta cataattcac catttattga agtatacatt
     8521 tctgtaactt ttattttttc tttttctgat actctaaccg aatctcgtaa atgtagtact
     8581 ccactataaa ggcgtatata tgcaagacgt tgtctttttt ttgtatattc aattttgaaa
     8641 acatttccgc aaagttcaga cggacctcga tgtgttgatg aataaaattt attagtaata
     8701 acttctataa ggttatcaat ccctatatta ctttttgcac ttccatgata aagagggaac
     8761 agagaacaat tctgaaatct tatgctttcc tcttgttcga gttccaatgc ttctaatgat
     8821 ttaccggaca tatatttctc taaaaggtca tcgtttccct ctattaccgt atcccattgt
     8881 tcagattcgg taaagttcgt cacacacaca ttaggataca gttctacctt ctgtttgatt
     8941 acaatttcgg cagaaagttt ctctttaata tcctgataaa ccgttgataa atcaattcca
     9001 ttttggtcaa tcttattgat aaaaaagatt gtgggaatcc ccattttcct aagtgcatga
     9061 aataatatac gagtttgtgc ttgtacgcca tcttttgcag aaatcagtag aattgcccca
     9121 tctaaaactg ataatgaacg atatacttct gctaagaaat ccatatgtcc tggcgtgtct
     9181 atgatgttca ccttcgtatt ttcccactga aaagaggtta ttcc
//
