LOCUS       sequence-192529-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of HA-RagA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Shen K, Choe A, Sabatini DM
  TITLE     Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 
            to Respond Rapidly to Amino Acid Availability.
  JOURNAL   Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026.
  PUBMED    29056322
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026."
COMMENT     Sequence Label: pRK5 HA-RagA
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aactgcactc ggttctatcg attgattccc gaccgacagt cggtctcttc accaaggcca
       61 ttcgcgccac catgggctac ccctatgatg tgcctgacta cgcagatctc aatggtggtg
      121 gtggtgggtc gacgatgcca aatacagcca tgaagaaaaa ggtgctgctg atggggaaga
      181 gcgggtcggg gaagaccagc atgaggtcga taatcttcgc caattacatt gctcgcgaca
      241 cccggcgcct gggggccacc attgacgtgg aacactccca cgtccgattc ctagggaacc
      301 tggtgctgaa cctgtgggac tgtggcggtc aggacacctt catggaaaat tacttcacca
      361 gccagcgaga caatatcttc cgtaacgtgg aagttttgat ttacgtgttt gacgtggaga
      421 gccgcgaact ggaaaaggac atgcattatt accagtcgtg tctggaggcc atcctccaga
      481 actctcctga cgccaaaatc ttctgcctgg tgcacaaaat ggatctggtt caggaggatc
      541 agcgtgacct gatttttaaa gagcgagagg aagacctgag gcgtctgtct cgcccgctgg
      601 agtgtgcttg ttttcgaacg tccatctggg atgagacgct ctacaaagcc tggtccagca
      661 tcgtctacca gctgattccc aacgttcagc agctggagat gaacctcagg aattttgccc
      721 aaatcattga ggccgatgaa gttctgctgt tcgaaagagc tacattcttg gttatttccc
      781 actaccagtg caaagagcag cgcgacgtcc accggtttga gaagatcagc aacatcatca
      841 aacagttcaa gctgagctgc agtaaattgg ccgcttcctt ccagagcatg gaagttagga
      901 attccaactt cgctgctttc atcgacatct tcacctcaaa tacgtacgtg atggtggtca
      961 tgtcagatcc gtcgatccct tctgcggcca ctctgatcaa cattcgcaat gcccggaaac
     1021 actttgagaa gctggagaga gtggatggcc ccaagcacag tctccttatg cgttgagcgg
     1081 ccgctaagta agtaaggatc cccagctgcc gccatg
//
