LOCUS       sequence-192531-        9544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing parBp-mKate2 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   van Raaphorst R, Kjos M, Veening JW
  TITLE     Chromosome segregation drives division site selection in 
            Streptococcus pneumoniae.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114.
  PUBMED    28674002
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114."
COMMENT     Sequence Label: pMK11-01
FEATURES             Location/Qualifiers
     source          1..9544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     67..89
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7389..7407)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7475..7579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7580..7648,7649..8440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7798..7817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8611..9199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9100..9119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9353..9370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9385..9525
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9451..9470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
       61 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
      121 agctagtctt cttttttacc tttagtaact actaatcctg cactcaaacc tagaagagct
      181 aaacctgctg ctactgctgc ttggcttgcc gcactacctg tatttggtaa ctgggcttta
      241 ttagtttgat cagcttcact tgaatcaatt ggttttgtat ctgctttttc tgacacttgt
      301 ggttttttag cttcttgagc tactggtttg gttccaacca agacgatgcg gtctgtcgga
      361 acttctacca cttcacggag tttttcttcc ttacttccat caggattaat cactgtaaag
      421 atacgttctt ttccaacttt tccttcttgt tctacacgag tttcacctag atacagtgtt
      481 gaatcttttt tctcaactgt cttgtaggcc aaatcttttt caacaaattc gagttttgga
      541 agatcttctt gtacagcaga aactggtttt tccttagtca actggatacg gtattccttg
      601 acttgttttc cactttctga aacgaggcga acaagtactg gaaggctatc ttctccacta
      661 tctaccacag ttgaagctac ttgattgttt tcttcaactg agacttttgg ccgttgacct
      721 ttataggtaa tttgatagtc ttgacgattt tcagcgaaat cagcaagttc ttttccatct
      781 acaagaatct ttgattgagt gctttcttga ggcaattcac ttggtgcaag gaaggtcatc
      841 tcaatcatcg caacaccgct cttgtcagct ttacgctcca tacgccatct catgactttg
      901 gctttgacag ctttaaatgt tacgttgatt tcatcaccag aaatagtaca taatggattt
      961 ccttacgcga aatacgggca gacatggcct gcccggttat tattattttt gacaccagac
     1021 caactggtaa tggtagcgac cggcgctcag cgatagcgct atcaaaagaa tcttgcttgg
     1081 caaggttctt ttttttgtct tttcaaaaaa gcacgaaaaa agcacccata aggtaaactt
     1141 ttgagtgctt tgctataaat acgcttcaca gtttcttctt catttttaac aaacaaaaaa
     1201 cccagctcat tgagctgggt ttaagctggc ttggcggcgt cctagtactg cagaattgcc
     1261 gaccttgact agtcacggtg tcccaattta ctaggcaaat cacaataacg tgcgacggct
     1321 acttcgtgct gttcaacata agtttcctta tctgcttctt tgatacgttc taaacgacga
     1381 tcgacgtagt aaacaccagg catcttaaga ttcttagctg gctttttaga acggtatgtg
     1441 gtcttcaaat tacagataag gtgacctcca ccgacaagtt tcaatgccat atcagcacga
     1501 ccttccaatc caccatcggc aggatataat gtttcggtag atgcttccca tccaagggtc
     1561 tttttctgca taacaggacc attacttgga aaattaactc cacggatctt tacattgtag
     1621 ataagacaac catcttgcaa agatgtatct tgagttgctg tcaatactcc accatcttca
     1681 taagttgtga cacgttccca tgtaaatcct tcaggaaatg actgtttaaa gaaatctggg
     1741 ataccttggg tatgattgat aaaagtcttt gatccgtaca taaaagatgt agcaagaata
     1801 tcaaaggcaa aaggcaatgg tccaccttca acggccttaa tacgcatggt ttgagtacct
     1861 tcatatggtt ttccttctcc ttctgaggta catttaaagt ggtgattatt tactgttcct
     1921 tccatgtaca atttcatgtg catattttcc ttgataagtt ctgatgagga tcctttagct
     1981 gcagcttctc ctccagatcc tctagatttc atcaacaact caatcaattt tttcttgatt
     2041 tcttgttctt cttccgttcc ctcaaactct aatttttcta attgagtttc aattttcttt
     2101 aactgcgtga ttggcttttt agcggttttc ttttgggggc taaatttagc cccctcttct
     2161 tcaccacctt gttctttagc ttttctaatc agtttttgag cggtataatg actgatacca
     2221 aatctttcgg caattaagtc cctagtcttt cctttaacag gttttccgtt gatttgtacc
     2281 ccttgttttt tggctttatc taaaatatcc atatagtcat caatgatggc tattttttct
     2341 tcttctggca tttttaagag cgagcgagtt tgtaggttag tttcatgcaa ctttagtctt
     2401 gttacaagct cgtcctcgtc ttcatcaact ttcttagcga gaacttcgta atcttcaggg
     2461 agtctatttt cactaattag ttttttaata gcattaaatc gtctatgccc tgcaatcaat
     2521 tcatagctat atttatcacg ttgtttaaca agaacgggtt gtaataagcc taactcgtct
     2581 atgctatcag ctaacttatc aatgtcaact aaatcatagt tgttattctc attttccttt
     2641 atctcctcaa taggaatatt ttttgtattt gttttttttc gcttgtgttc gtccttgttc
     2701 ataaggtctg taaatccgaa actattactc atgtttgtcc tccttattag ttaatcagta
     2761 ctcgagatcc tatgcggccg ctggaagctt tacccgggat tggaattctc atatttgcct
     2821 ccttaagatc cgcagactca gaatagactc attctttgtc tttcccttac caaccaccag
     2881 actcgttaac agattgatag taatcgcaat aattcctaac cagagaatcc cctcatcatt
     2941 gactggttgc ggatgcaaaa tcttcgtgac attttccaaa atgactagaa cagagcccgt
     3001 tacgagaatc acagctgtta ctaaggctcc tagcaggcta aaccgcttat agcccaaggt
     3061 gtactgattg tcttcttcac gattggagat tgtttctaga aaagctgata ttccaattgc
     3121 aatcgcatct cccaagtcat gcacagagtc agcaagaaca gcgctagaac caaatactcc
     3181 acctgcaata aactcaacaa tggcataagt caaatttaag aaaaaagcca cccaaacagc
     3241 atattttgcc ttcatatttc tcattccttt gttataatag atttatgaac accttgttca
     3301 ttatcattat ccactaaaac tcaaagaaag gatagaagca aaacgtcagc tttattcagt
     3361 tctgaacaat ttgccttaag tgtccatatg actaacattg accgccgtat cagcagcatg
     3421 cggtaccgtc ggctgcatgc atcgatagat ccacaggacg ggtgtggtcg ccatgatcgc
     3481 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
     3541 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcca
     3601 agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc
     3661 gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt
     3721 ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc
     3781 ctttacaaat atgctcttac gtgctataat ttaagtatct atttaaaagg agttaataaa
     3841 tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa ataattggat
     3901 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
     3961 ttctaaagta tccggagaat atctgtatgc tttgtatgcc tatggttatg cataaaaatc
     4021 ccagtgataa gagtatttat cactgggatt tttatgccct tttgggcttt tgaatggagg
     4081 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
     4141 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
     4201 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
     4261 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
     4321 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
     4381 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
     4441 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
     4501 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
     4561 tagaactgta tcctaatgtg tgtgtgacga actttaccga atctgaacaa tgggatacgg
     4621 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
     4681 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
     4741 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
     4801 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
     4861 atacaaaaaa aagacaacgt cttgcatata tacgccttta tagtggagta ctacatttac
     4921 gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa
     4981 taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa
     5041 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
     5101 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
     5161 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
     5221 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
     5281 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag
     5341 tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc
     5401 caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa
     5461 tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta
     5521 tggaagggat acgctatggt tgtgaacaag gattgtatgg ttggaatgtg acggactgta
     5581 aaatctgttt taagtatggc ttatactata gccctgttag taccccagca gattttcgga
     5641 tgcttgctcc tattgtattg gagcaagcct ttagaaaagc tggaacagaa ttgttagagc
     5701 catatcttag ttttaaagtt tatgcaccac aggaatatct ttcacgggca tataacgatg
     5761 ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag gtcattatta
     5821 ttggagaaat tcctgctcga tgtattcaag attatcgcaa tgatttaact ttttttacaa
     5881 atgggcttag tgtttgttta gcagagctaa aaggatatca ggttaccact ggcgaacctg
     5941 tttgccagac ccgtcgtcta aatagtcaga tagataaagt aagatatatg ttcaataaaa
     6001 taacttagtg cgttttatgt tgttatataa atatggtttc ttattaaata agatgaaata
     6061 ttctttaata tagatttgaa ttaaagtgga aaggaggaga ttgttattat aaactacaag
     6121 tggatattgt gtcctatttg tggaaataaa acaagactac gaatacgagt ggatactata
     6181 cttaaaaatt tccctttata ctgccccaaa tgtaagaacg aaactttaat taatgttcaa
     6241 aaaatgaata taataacaat caaagagcca gacgccaaga cgcagagccg ataatttgag
     6301 aaatgaaact ctcatcttat cggctctttt tgtttatctg aattttactg actagccttc
     6361 aatatttctt gcttcattga taccttttgc tagtgcttcc attaaggata gttctttgtc
     6421 tgtaaagcta gcggagcgca ttattttgct tttgctgcgt actcttcgtt acgtttgatc
     6481 atttgttttc tgtaccaagc aaagataccg atatagaata caaggaaggc tactgcacca
     6541 aggattgctt tgatatcacc agttgtagtg ttaccaattg tccaaccaag aagtttttcg
     6601 attggtcctt caagagtaga gtgagtaatc aattgagttt ggctcacacc ttctgggaag
     6661 gcacctacac ctttagcaag ttctgttgca aatggtgcaa taagtgtacc tgaaagaagg
     6721 aagagtggca acaagagtgt tccgaagata atcatacgga gcaatttacc acgagttaca
     6781 accaagagag ctggagtaac acccatagcg atgatacctg caagtggcaa gataccattt
     6841 ccaacttttg aaagaagcac tgcttcaatc aacatgattg gtgcaagtac gttggcacaa
     6901 gcccagattt cagcacgacc agcgatgaat ggccagtcaa gaccgatatt gaatttacgt
     6961 ccttgaagac gtttagtagc aacgtttgta ataccttgtg atagtggttc tacggctgcg
     7021 atgaaccatg aaccgataag tgagaagagt tccaaagata caccggcagt caaaccaaga
     7081 gacaaccatc ctttgataac aagacgccat ttatctgcat ctgcaacacc tgcaattgga
     7141 tgtggagttc ccataatacc gataacgata ccaaggatga aaccgatgaa gaatttagat
     7201 ccccagaaac cgattttctt gttcaattta gcagcatcaa agtcatattt atcaaggcct
     7261 gggaagaatt tttcaaaaat cttatccaaa accatgataa ctgggttcat catgtagttc
     7321 atgtgagttg atgtcattgg tgatgaactt ggggcgttaa gaaggaatta attcttgaag
     7381 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7441 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7561 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7621 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7681 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7741 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7801 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     7861 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7921 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7981 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8041 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8101 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8161 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8221 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8281 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8341 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8401 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9301 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9361 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     9421 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9481 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     9541 acac
//
