LOCUS       sequence-192533-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing parBp(mut)-mKate2 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   van Raaphorst R, Kjos M, Veening JW
  TITLE     Chromosome segregation drives division site selection in 
            Streptococcus pneumoniae.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114.
  PUBMED    28674002
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114."
COMMENT     Sequence Label: pMK11-02
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3441..3463)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3549..3689
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3604..3623
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3704..3721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3875..4463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3955..3974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4634..5425,5426..5494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5257..5276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5495..5599)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5667..5685
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgttgatgca atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc
       61 gcccagtcct gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca
      121 cacccgtcct gtggatctat cgatgcatgc agccgacggt accgcatgct gctgatacgg
      181 cggtcaatgt tagtcatatg gacacttaag gcaaattgtt cagaactgaa taaagctgac
      241 gttttgcttc tatcctttct ttgagtttta gtggataatg ataatgaaca aggtgttcat
      301 aaatctatta taacaaagga atgagaaata tgaaggcaaa atatgctgtt tgggtggctt
      361 ttttcttaaa tttgacttat gccattgttg agtttattgc aggtggagta tttggttcta
      421 gcgctgttct tgctgactct gtgcatgact tgggagatgc gattgcaatt ggaatatcag
      481 cttttctaga aacaatctcc aatcgtgaag aagacaatca gtacaccttg ggctataagc
      541 ggtttagcct gctaggagcc ttagtaacag ctgtgattct cgtaacgggc tctgttctag
      601 tcattttgga aaatgtcacg aagattttgc atccgcaacc agtcaatgat gaggggattc
      661 tctggttagg aattattgcg attactatca atctgttaac gagtctggtg gttggtaagg
      721 gaaagacaaa gaatgagtct attctgagtc tgcggatctt aaggaggcaa atatgagaat
      781 tccaatcccg ggtaaagctt ccagcggccg cataggatct cgagtactga ttaactaata
      841 aggaggacaa acatgagtaa tagtttcgga tttacagacc ttatgaacaa ggacgaacac
      901 aagcgaaaaa aaacaaatac aaaaaatatt cctattgagg agataaagga aaatgagaat
      961 aacaactatg atttagttga cattgataag ttagctgata gcatagacga gttaggctta
     1021 ttacaacccg ttcttgttaa acaacgtgat aaatatagct atgaattgat tgcagggcat
     1081 agacgattta atgctattaa aaaactaatt agtgaaaata gactccctga agattacgaa
     1141 gttctcgcta agaaagttga tgaagacgag gacgagcttg taacaagact aaagttgcat
     1201 gaaactaacc tacaaactcg ctcgctctta aaaatgccag aagaagaaaa aatagccatc
     1261 attgatgact atatggatat tttagataaa gccaaaaaac aaggggtaca aatcaacgga
     1321 aaacctgtta aaggaaagac tagggactta attgccgaaa gatttggtat cagtcattat
     1381 accgctcaaa aactgattag aaaagctaaa gaacaaggtg gtgaagaaga gggagcaaag
     1441 ttctctccac aaaagaaaac cgctaaaaag ccaatcacgc agttaaagaa aattgaaact
     1501 caattagaaa aattagagtt tgagggaacg gaagaagaac aagaaatcaa gaaaaaattg
     1561 attgagttgt tgatgaaatc tagaggatct ggaggagaag ctgcagctaa aggatcctca
     1621 tcagaactta tcaaggaaaa tatgcacatg aaattgtaca tggaaggaac agtaaataat
     1681 caccacttta aatgtacctc agaaggagaa ggaaaaccat atgaaggtac tcaaaccatg
     1741 cgtattaagg ccgttgaagg tggaccattg ccttttgcct ttgatattct tgctacatct
     1801 tttatgtacg gatcaaagac ttttatcaat catacccaag gtatcccaga tttctttaaa
     1861 cagtcatttc ctgaaggatt tacatgggaa cgtgtcacaa cttatgaaga tggtggagta
     1921 ttgacagcaa ctcaagatac atctttgcaa gatggttgtc ttatctacaa tgtaaagatc
     1981 cgtggagtta attttccaag taatggtcct gttatgcaga aaaagaccct tggatgggaa
     2041 gcatctaccg aaacattata tcctgccgat ggtggattgg aaggtcgtgc tgatatggca
     2101 ttgaaacttg tcggtggagg tcaccttatc tgtaatttga agaccacata ccgttctaaa
     2161 aagccagcta agaatcttaa gatgcctggt gtttactacg tcgatcgtcg tttagaacgt
     2221 atcaaagaag cagataagga aacttatgtt gaacagcacg aagtagccgt cgcacgttat
     2281 tgtgatttgc ctagtaaatt gggacaccgt gactagtcaa ggtcggcaat tctgcagtac
     2341 taggacgccg ccaagccagc ttaaacccag ctcaatgagc tgggtttttt gtttgttaaa
     2401 aatgaagaag aaactgtgaa gcgtatttat agcaaagcac tcaaaagttt accttatggg
     2461 tgcttttttc gtgctttttt gaaaagacaa aaaaaagaac cttgccaagc aagattcttt
     2521 tgatagcgct atcgctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     2581 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
     2641 atttctggtg atgaaatcaa cgtaacattt aaagctgtca aagccaaagt catgagatgg
     2701 cgtatggagc gtaaagctga caagagcggt gttgcgatga ttgagatgac cttccttgca
     2761 ccaagtgaat tgcctcaaga aagcactcaa tcaaagattc ttgtagatgg aaaagaactt
     2821 gctgatttcg ctgaaaatcg tcaagactat caaattacct ataaaggtca acggccaaaa
     2881 gtctcagttg aagaaaacaa tcaagtagct tcaactgtgg tagatagtgg agaagatagc
     2941 cttccagtac ttgttcgcct cgtttcagaa agtggaaaac aagtcaagga ataccgtatc
     3001 cagttgacta aggaaaaacc agtttctgct gtacaagaag atcttccaaa actcgaattt
     3061 gttgaaaaag atttggccta caagacagtt gagaaaaaag attcaacact gtatctaggt
     3121 gaaactcgtg tagaacaaga aggaaaagtt ggaaaagaac gtatctttac agtgattaat
     3181 cctgatggaa gtaaggaaga aaaactccgt gaagtggtag aagttccgac agaccgcatc
     3241 gtcttggttg gaaccaaacc agtagctcaa gaagctaaaa aaccacaagt gtcagaaaaa
     3301 gcagatacaa aaccaattga ttcaagtgaa gctgatcaaa ctaataaagc ccagttacca
     3361 aatacaggta gtgcggcaag ccaagcagca gtagcagcag gtttagcctg cctcgcgcgt
     3421 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3481 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3541 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     3601 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3661 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     3721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3961 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4021 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4081 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4141 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4201 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4261 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4321 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4381 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4441 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4501 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4561 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4921 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     4981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5221 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     5281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     5641 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     5701 aattaattcc ttcttaacgc cccaagttca tcaccaatga catcaactca catgaactac
     5761 atgatgaacc cagttatcat ggttttggat aagatttttg aaaaattctt cccaggcctt
     5821 gataaatatg actttgatgc tgctaaattg aacaagaaaa tcggtttctg gggatctaaa
     5881 ttcttcatcg gtttcatcct tggtatcgtt atcggtatta tgggaactcc acatccaatt
     5941 gcaggtgttg cagatgcaga taaatggcgt cttgttatca aaggatggtt gtctcttggt
     6001 ttgactgccg gtgtatcttt ggaactcttc tcacttatcg gttcatggtt catcgcagcc
     6061 gtagaaccac tatcacaagg tattacaaac gttgctacta aacgtcttca aggacgtaaa
     6121 ttcaatatcg gtcttgactg gccattcatc gctggtcgtg ctgaaatctg ggcttgtgcc
     6181 aacgtacttg caccaatcat gttgattgaa gcagtgcttc tttcaaaagt tggaaatggt
     6241 atcttgccac ttgcaggtat catcgctatg ggtgttactc cagctctctt ggttgtaact
     6301 cgtggtaaat tgctccgtat gattatcttc ggaacactct tgttgccact cttccttctt
     6361 tcaggtacac ttattgcacc atttgcaaca gaacttgcta aaggtgtagg tgccttccca
     6421 gaaggtgtga gccaaactca attgattact cactctactc ttgaaggacc aatcgaaaaa
     6481 cttcttggtt ggacaattgg taacactaca actggtgata tcaaagcaat ccttggtgca
     6541 gtagccttcc ttgtattcta tatcggtatc tttgcttggt acagaaaaca aatgatcaaa
     6601 cgtaacgaag agtacgcagc aaaagcaaaa taatgcgctc cgctagcttt acagacaaag
     6661 aactatcctt aatggaagca ctagcaaaag gtatcaatga agcaagaaat attgaaggct
     6721 agtcagtaaa attcagataa acaaaaagag ccgataagat gagagtttca tttctcaaat
     6781 tatcggctct gcgtcttggc gtctggctct ttgattgtta ttatattcat tttttgaaca
     6841 ttaattaaag tttcgttctt acatttgggg cagtataaag ggaaattttt aagtatagta
     6901 tccactcgta ttcgtagtct tgttttattt ccacaaatag gacacaatat ccacttgtag
     6961 tttataataa caatctcctc ctttccactt taattcaaat ctatattaaa gaatatttca
     7021 tcttatttaa taagaaacca tatttatata acaacataaa acgcactaag ttattttatt
     7081 gaacatatat ctcactttat ctatctgact atttagacga cgggtctggc aaacaggttc
     7141 gccagtggta acctgatatc cttttagctc tgctaaacaa acactaagcc catttgtaaa
     7201 aaaagttaaa tcattgcgat aatcttgaat acatcgagca ggaatttctc caataataat
     7261 gacctcatta tttttcagtt gagtatttac gatatttgca caatatttgg gagcatcgtt
     7321 atatgcccgt gaaagatatt cctgtggtgc ataaacttta aaactaagat atggctctaa
     7381 caattctgtt ccagcttttc taaaggcttg ctccaataca ataggagcaa gcatccgaaa
     7441 atctgctggg gtactaacag ggctatagta taagccatac ttaaaacaga ttttacagtc
     7501 cgtcacattc caaccataca atccttgttc acaaccatag cgtatccctt ccataactgc
     7561 attttgaaat gattgattta agtatccaag agaaaccgag ctctcatact gcattccact
     7621 tcccaacgga agcggtgata cagataaacc aatggaagcc cagaaaggat ttggcggcac
     7681 ttcgatgtga atggtatatt ctgcattttt taacggtctc tccatataaa tgactgtagg
     7741 ctcttttagt tctatctcca catgatactt ttcttgcaac agtgcactaa tcacttccat
     7801 ttgtactttc cctaagaaag aaagtataat ttcatgtgtc gtagaatcca cgtaatatcg
     7861 tagaagcgga tcactatctg agatttccaa aagggcatca agcaacattt ctctctgttc
     7921 aggtttactc ggttcaacag ttgtttgtag tagagggtgc ggattttcaa tcttttttct
     7981 ctgtggcaat agttttgtat ctccaagaac actatttaac ttcaaaagct cattttgcaa
     8041 aataacaatt tctccagaat aagctcgatc aatcttacat aattcaccat ttattgaagt
     8101 atacatttct gtaactttta ttttttcttt ttctgatact ctaaccgaat ctcgtaaatg
     8161 tagtactcca ctataaaggc gtatatatgc aagacgttgt cttttttttg tatattcaat
     8221 tttgaaaaca tttccgcaaa gttcagacgg acctcgatgt gttgatgaat aaaatttatt
     8281 agtaataact tctataaggt tatcaatccc tatattactt tttgcacttc catgataaag
     8341 agggaacaga gaacaattct gaaatcttat gctttcctct tgttcgagtt ccaatgcttc
     8401 taatgattta ccggacatat atttctctaa aaggtcatcg tttccctcta ttaccgtatc
     8461 ccattgttca gattcggtaa agttcgtcac acacacatta ggatacagtt ctaccttctg
     8521 tttgattaca atttcggcag aaagtttctc tttaatatcc tgataaaccg ttgataaatc
     8581 aattccattt tggtcaatct tattgataaa aaagattgtg ggaatcccca ttttcctaag
     8641 tgcatgaaat aatatacgag tttgtgcttg tacgccatct tttgcagaaa tcagtagaat
     8701 tgccccatct aaaactgata atgaacgata tacttctgct aagaaatcca tatgtcctgg
     8761 cgtgtctatg atgttcacct tcgtattttc ccactgaaaa gaggttattc ctgtctgaat
     8821 tgtaattcct ctctgacgtt ctaaaagcgt attatccgtc ctcgttgtac ctttgtccac
     8881 gcttcctaat tctgtaatcg ctccactgtt atataataag ctttctgtta aggtagtttt
     8941 tcctgcatca acatgagcta aaactccaat attaataatt ttcatgtgat tttcctccat
     9001 tcaaaagccc aaaagggcat aaaaatccca gtgataaata ctcttatcac tgggattttt
     9061 atgcataacc ataggcatac aaagcataca gatattctcc ggatacttta gaatcacatg
     9121 ataaaggtat tcttaaactg gggacaaaaa actaagccct cctaaaaaag gacgtccaat
     9181 tatttgttcc cgctatcaaa ttgacagttt atttaagaat accttgccgc atatttatta
     9241 actcctttta aatagatact taaattatag cacgtaagag catatttgta aaggaatctc
     9301 caatttttta tcaaaaagag tacatgatta caaagtatct gtaatcatgt accaatattt
     9361 attattttac aatcttccaa ttatcttgtt tttctaatat caactcaaat tgcgagattt
     9421 gggttgcctt tgtttcttga tctaaatatt tcacagcgac attcacgatt acttggctag
     9481 cgctatatg
//
