LOCUS       sequence-192535-       10526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert the Tre-Tight or UAS ifgMosaic Donor vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: pRosa26-Acceptor (AG103)
FEATURES             Location/Qualifiers
     source          1..10526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2263..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2891..2909
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3356..3372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3382..3400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3419..4501
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(4508..4573,4574..4611)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    4677..4724
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(4907..4924)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(5096..5112)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5125..5142)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5131..5355
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5445..9785
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(9836..9852)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9885..9903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9913..9929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10071..10499
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     10368..10389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcacgtgt tcgaaacgcg ttgacattga ttattgacta
     2281 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
     2881 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
     2941 gcttgtgacg ttggagtcca cgttctttaa tagtggactc ttgttgaacc atcaccctaa
     3001 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3061 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3121 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3181 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacwttcag gctgcgcaac
     3241 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     3301 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     3361 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc
     3421 ggcaggccct ccgagcgtgg tggagccgtt ctgtgagaca gccgggtacg agtcgtgacg
     3481 ctggaagggg caagcgggtg gtgggcagga atgcggtccg ccctgcagca accggagggg
     3541 gagggagaag ggagcggaaa agtctccacc ggacgcggcc atggctcggg gggggggggg
     3601 cagcggagga gcgcttccgg ccgacgtctc gtcgctgatt ggcttctttt cctcccgccg
     3661 tgtgtgaaaa cacaaatggc gtgttttggt tggcgtaagg cgcctgtcag ttaacggcag
     3721 ccggagtgcg cagccgccgg cagcctcgct ctgcccactg ggtggggcgg gaggtaggtg
     3781 gggtgaggcg agctggacgt gcgggcgcgg tcggcctctg gcggggcggg ggaggggagg
     3841 gagggtcagc gaaagtagct cgcgcgcgag cggccgccca ccctcccctt cctctggggg
     3901 agtcgtttta cccgccgccg gccgggcctc gtcgtctgat tggctctcgg ggcccagaaa
     3961 actggccctt gccattggct cgtgttcgtg caagttgagt ccatccgccg gccagcgggg
     4021 gcggcgagga ggcgctccca ggttccggcc ctcccctcgg ccccgcgccg cagagtctgg
     4081 ccgcgcgccc ctgcgcaacg tggcaggaag cgcgcgctgg gggcggggac gggcagtagg
     4141 gctgagcggc tgcggggcgg gtgcaagcac gtttccgact tgagttgcct caagaggggc
     4201 gtgctgagcc agacctccat cgcgcactcc ggggagtgga gggaaggagc gagggctcag
     4261 ttgggctgtt ttggaggcag gaagcacttg ctctcccaaa gtcgctctga gttgttatca
     4321 gtaagggagc tgcagtggag taggcgggga gaaggccgca cccttctccg gaggggggag
     4381 gggagtgttg caataccttt ctgggagttc tctgctgcct cctggcttct gaggaccgcc
     4441 ctgggcctgg gagaatccct tccccctctt ccctcgtgat ctgcaactcc agtctttcta
     4501 gcatctgtag ggcgcagtag tccagggttt ccttgatgat gtcatactta tcctgtccct
     4561 tttttttcca cagctcgcgg ttgaggacaa actcttcgcg gtctttccag tggttaatgc
     4621 acgccggcgc acattaatta actctggcgc gccgcggccg cgatcaattc ggtaccgaag
     4681 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcctcgag ggtaccccaa
     4741 ttccgccccc cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4801 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4861 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4921 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4981 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5041 tgcggccaaa agccaccgag ctcggatcca ctagtccagt gtggtggaat tgggggatcc
     5101 actagttcta gagcggccga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5161 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5221 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5281 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5341 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgaga tccactagtt
     5401 ctagcctcga ggctagagcg gccgccgcgc cgatatcgaa ttcgctagaa gatgggcggg
     5461 agtcttctgg gcaggcttaa aggctaacct ggtgtgtggg cgttgtcctg caggggaatt
     5521 gaacaggtgt aaaattggag ggacaagact tcccacagat tttcggtttt gtcgggaagt
     5581 tttttaatag gggcaaataa ggaaaatggg aggataggta gtcatctggg gttttatgca
     5641 gcaaaactac aggttattat tgcttgtgat ccgcctcgga gtattttcca tcgaggtaga
     5701 ttaaagacat gctcacccga gttttatact ctcctgcttg agatccttac tacagtatga
     5761 aattacagtg tcgcgagtta gactatgtaa gcagaatttt aatcattttt aaagagccca
     5821 gtacttcata tccatttctc ccgctccttc tgcagcctta tcaaaaggta ttttagaaca
     5881 ctcattttag ccccattttc atttattata ctggcttatc caacccctag acagagcatt
     5941 ggcattttcc ctttcctgat cttagaagtc tgatgactca tgaaaccaga cagattagtt
     6001 acatacacca caaatcgagg ctgtagctgg ggcctcaaca ctgcagttct tttataactc
     6061 cttagtacac tttttgttga tcctttgcct tgatccttaa ttttcagtgt ctatcacctc
     6121 tcccgtcagg tggtgttcca catttgggcc tattctcagt ccagggagtt ttacaacaat
     6181 agatgtattg agaatccaac ctaaagctta actttccact cccatgaatg cctctctcct
     6241 ttttctccat ttataaactg agctattaac cattaatggt ttccaggtgg atgtctcctc
     6301 ccccaatatt acctgatgta tcttacatat tgccaggctg atattttaag acattaaaag
     6361 gtatatttca ttattgagcc acatggtatt gattactgct tactaaaatt ttgtcattgt
     6421 acacatctgt aaaaggtggt tccttttgga atgcaaagtt caggtgtttg ttgtctttcc
     6481 tgacctaagg tcttgtgagc ttgtattttt tctatttaag cagtgctttc tcttggactg
     6541 gcttgactca tggcattcta cacgttattg ctggtctaaa tgtgattttg ccaagcttct
     6601 tcaggaccta taattttgct tgacttgtag ccaaacacaa gtaaaatgat taagcaacaa
     6661 atgtatttgt gaagcttggt ttttaggttg ttgtgttgtg tgtgcttgtg ctctataata
     6721 atactatcca ggggctggag aggtggctcg gagttcaaga gcacagactg ctcttccaga
     6781 agtcctgagt tcaattccca gcaaccacat ggtggctcac aaccatctgt aatgggatct
     6841 gatgccctct tctggtgtgt ctgaagacca caagtgtatt cacattaaat aaataaatcc
     6901 tccttcttct tctttttttt ttttttaaag agaatactgt ctccagtaga atttactgaa
     6961 gtaatgaaat actttgtgtt tgttccaata tggtagccaa taatcaaatt actctttaag
     7021 cactggaaat gttaccaagg aactaatttt tatttgaagt gtaactgtgg acagaggagc
     7081 cataactgca gacttgtggg atacagaaga ccaatgcaga ctttaatgtc ttttctctta
     7141 cactaagcaa taaagaaata aaaattgaac ttctagtatc ctatttgttt aaactgctag
     7201 ctttacttaa cttttgtgct tcatctatac aaagctgaaa gctaagtctg cagccattac
     7261 taaacatgaa agcaagtaat gataattttg gatttcaaaa atgtagggcc agagtttagc
     7321 cagccagtgg tggtgcttgc ctttatgcct ttaatcccag cactctggag gcagagacag
     7381 gcagatctct gagtttgagc ccagcctggt ctacacatca agttctatct aggatagcca
     7441 ggaatacaca cagaaaccct gttggggagg ggggctctga gatttcataa aattataatt
     7501 gaagcattcc ctaatgagcc actatggatg tggctaaatc cgtctacctt tctgatgaga
     7561 tttgggtatt attttttctg tctctgctgt tggttgggtc ttttgacact gtgggctttc
     7621 tttaaagcct ccttcctgcc atgtggtctc ttgtttgcta ctaacttccc atggcttaaa
     7681 tggcatggct ttttgccttc taagggcagc tgctgagatt tgcagcctga tttccagggt
     7741 ggggttggga aatctttcaa acactaaaat tgtcctttaa tttttttttt aaaaaatggg
     7801 ttatataata aacctcataa aatagttatg aggagtgagg tggactaata ttaaatgagt
     7861 ccctccccta taaaagagct attaaggctt tttgtcttat acttaacttt ttttttaaat
     7921 gtggtatctt tagaaccaag ggtcttagag ttttagtata cagaaactgt tgcatcgctt
     7981 aatcagattt tctagtttca aatccagaga atccaaattc ttcacagcca aagtcaaatt
     8041 aagaatttct gacttttaat gttaatttgc ttactgtgaa tataaaaatg atagcttttc
     8101 ctgaggcagg gtctcactat gtatctctgc ctgatctgca acaagatatg tagactaaag
     8161 ttctgcctgc ttttgtctcc tgaatactaa ggttaaaatg tagtaatact tttggaactt
     8221 gcaggtcaga ttcttttata ggggacacac taagggagct tgggtgatag ttggtaaaat
     8281 gtgtttcaag tgatgaaaac ttgaattatt atcaccgcaa cctacttttt aaaaaaaaaa
     8341 gccaggcctg ttagagcatg cttaagggat ccctaggact tgctgagcac acaagagtag
     8401 ttacttggca ggctcctggt gagagcatat ttcaaaaaac aaggcagaca accaagaaac
     8461 tacagttaag gttacctgtc tttaaaccat ctgcatatac acagggatat taaaatattc
     8521 caaataatat ttcattcaag ttttccccca tcaaattggg acatggattt ctccggtgaa
     8581 taggcagagt tggaaactaa acaaatgttg gttttgtgat ttgtgaaatt gttttcaagt
     8641 gatagttaaa gcccatgaga tacagaacaa agctgctatt tcgaggtctc ttggtttata
     8701 ctcagaagca cttctttggg tttccctgca ctatcctgat catgtgctag gcctacctta
     8761 ggctgattgt tgttcaaata aacttaagtt tcctgtcagg tgatgtcata tgatttcata
     8821 tatcaaggca aaacatgtta tatatgttaa acatttgtac ttaatgtgaa agttaggtct
     8881 ttgtgggttt gatttttaat tttcaaaacc tgagctaaat aagtcatttt tacatgtctt
     8941 acatttggtg gaattgtata attgtggttt gcaggcaaga ctctctgacc tagtaaccct
     9001 acctatagag cactttgctg ggtcacaagt ctaggagtca agcatttcac cttgaagttg
     9061 agacgttttg ttagtgtata ctagtttata tgttggagga catgtttatc cagaagatat
     9121 tcaggactat ttttgactgg gctaaggaat tgattctgat tagcactgtt agtgagcatt
     9181 gagtggcctt taggcttgaa ttggagtcac ttgtatatct caaataatgc tggccttttt
     9241 taaaaagccc ttgttcttta tcaccctgtt ttctacataa tttttgttca aagaaatact
     9301 tgtttggatc tccttttgac aacaatagca tgttttcaag ccatattttt tttccttttt
     9361 tttttttttt ttggtttttc gagacagggt ttctctgtat agccctggct gtcctggaac
     9421 tcactttgta gaccaggctg gcctcgaact cagaaatccg cctgcctctg cctcctgagt
     9481 gccgggatta aaggcgtgca ccaccacgcc tggctaagtt ggatattttg ttatataact
     9541 ataaccaata ctaactccac tgggtggatt tttaattcag tcagtagtct taagtggtct
     9601 ttattggccc ttcattaaaa tctactgttc actctaacag aggctgttgg tactagtggc
     9661 acttaagcaa cttcctacgg atatactagc agattaaggg tcagggatag aaactagtct
     9721 agcgttttgt atacctacca gctttatact accttgttct gatagaaata tttcaggaca
     9781 tctagcttat cgataccgtc gacgttcgaa cacgtggaat tcctgcagcc cgggggatcc
     9841 actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta
     9901 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     9961 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    10021 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtactg
    10081 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    10141 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    10201 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    10261 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    10321 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    10381 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    10441 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
    10501 taacaaaata ttaacgctta caattt
//
