LOCUS       sequence-19259-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf182
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             481..522
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             532..549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(572..589)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    578..689
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctgaccac catgaagaac gtactgatta ttttcggtaa accgtattgt agtatttgtg
       61 aaaatgtcag tgatgcagta gaagaattaa aatccgagta tgatatactc catgttgata
      121 tcttatcatt ttttttaaag gatggtgatt caagtatgct gggtgacgtg aagcgcggaa
      181 ccctaatagg aaacttcgca gcgcatctat ctaactacat cgtttccatt ttcaaataca
      241 atccacagac aaaacaaatg gcatttgtgg acattaataa atccttggat ttcaccaaaa
      301 cagataaatc gctggtgaat ttggaaattc taaaatccga aatagaaaaa gctacctatg
      361 gagtttggcc acctgttacc gaataagcaa ttcccgggga tccgaataaa gggtcaagac
      421 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
      481 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac
      541 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      601 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      661 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttcta
//
