LOCUS       sequence-192591-       11537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 13 of murine
            Bcan and intron 10 of murine Ntrk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Cook PJ, Thomas R, Kannan R, de Leon ES, Drilon A, Rosenblum MK, 
            Scaltriti M, Benezra R, Ventura A
  TITLE     Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent 
            glioma driver and therapeutic target.
  JOURNAL   Nat Commun. 2017 Jul 11;8:15987. doi: 10.1038/ncomms15987.
  PUBMED    28695888
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-07-11"; volume: "8:15987. doi"; pages: " 
            10.1038/ncomms15987"
COMMENT     Sequence Label: Adeno BN
FEATURES             Location/Qualifiers
     source          1..11537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   16..118
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     206..356
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        406..654
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        675..750
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        841..1089
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1111..1186
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1276..1561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1563..1840
                     /label=chicken beta-actin promoter
     intron          1841..2069
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2081..2090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2090..2155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2162..2182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2207..6307
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2207..6307
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6308..6355
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6378..6394)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6378..6394)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6414..6548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6438..6457)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6492..6511
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9790..10378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9870..9889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10549..11340,11341..11409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11172..11191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11410..11514)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
       61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
      121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
      181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
      241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
      301 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatattt
      361 gtctagggag atccggtacc gtttaaactc gagaatggcg catgtgaggg cctatttccc
      421 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
      481 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      541 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      601 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcatgc
      661 agttgaccaa gctcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      721 caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga aatagcaagt
      781 taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa tggctctaga
      841 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
      901 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      961 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     1021 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     1081 cgaaacaccg tggagctctg gagacctcgt gttttagagc tagaaatagc aagttaaaat
     1141 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
     1201 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
     1261 gacaaatggg gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1321 cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg
     1381 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1441 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat
     1501 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1561 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     1621 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     1681 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     1741 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     1801 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc
     1861 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     1921 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga
     1981 gcaagaggta agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg
     2041 agcacctgcc tgaaatcact ttttttcagg ttggaccggt gccaccatgg actataagga
     2101 ccacgacgga gactacaagg atcatgatat tgattacaaa gacgatgacg ataagatggc
     2161 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag
     2221 catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     2281 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     2341 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
     2401 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     2461 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     2521 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     2581 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     2641 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     2701 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     2761 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     2821 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     2881 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     2941 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     3001 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     3061 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     3121 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     3181 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     3241 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     3301 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     3361 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     3421 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     3481 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     3541 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     3601 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     3661 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     3721 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     3781 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     3841 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     3901 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     3961 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     4021 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     4081 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     4141 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     4201 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     4261 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     4321 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     4381 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     4441 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     4501 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     4561 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     4621 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     4681 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     4741 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     4801 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     4861 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     4921 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     4981 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     5041 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     5101 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     5161 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     5221 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     5281 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     5341 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     5401 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     5461 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     5521 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     5581 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     5641 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     5701 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     5761 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     5821 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     5881 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     5941 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     6001 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     6061 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     6121 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     6181 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     6241 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     6301 aggcgacaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagtaaga
     6361 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg ggagatccag
     6421 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     6481 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6541 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcasggg gaggtgtggg
     6601 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcccggct
     6661 gcctcgcgcg tttcggtgat gacggtgaaa acctcttgac acatgcagct cccggagacg
     6721 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6781 ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagtccccgc ggtggcagat
     6841 ctggaaggtg ctgaggtacg atgagacccg caccaggtgc agaccctgcg agtgtggcgg
     6901 taaacatatt aggaaccagc ctgtgatgct ggatgtgacc gaggagctga ggcccgatca
     6961 cttggtgctg gcctgcaccc gcgctgagtt tggctctagc gatgaagata cagattgagg
     7021 tactgaaatg tgtgggcgtg gcttaagggt gggaaagaat atataaggtg ggggtcttat
     7081 gtagttttgt atctgttttg cagcagccgc cgccgccatg agcaccaact cgtttgatgg
     7141 aagcattgtg agctcatatt tgacaacgcg catgccccca tgggccgggg tgcgtcagaa
     7201 tgtgatgggc tccagcattg atggtcgccc cgtcctgccc gcaaactcta ctaccttgac
     7261 ctacgagacc gtgtctggaa cgccgttgga gactgcagcc tccgccgccg cttcagccgc
     7321 tgcagccacc gcccgcggga ttgtgactga ctttgctttc ctgagcccgc ttgcaagcag
     7381 tgcagcttcc cgttcatccg cccgcgatga caagttgacg gctcttttgg cacaattgga
     7441 ttctttgacc cgggaactta atgtcgtttc tcagcagctg ttggatctgc gccagcaggt
     7501 ttctgccctg aaggcttcct cccctcccaa tgcggtttaa aacataaata aaaaaccaga
     7561 ctctgtttgg atttggatca agcaagtgtc ttgctgtctt tatttagggg ttttgcgcgc
     7621 gcggtaggcc cgggaccagc ggtctcggtc gttgagggtc ctgtgtattt tttccaggac
     7681 gtggtaaagg tgactctgga tgttcagata catgggcata agcccgtctc tggggtggag
     7741 gtagcaccac tgcagagctt catgctgcgg ggtggtgttg tagatgatcc agtcgtagca
     7801 ggagcgctgg gcgtggtgcc taaaaatgtc tttcagtagc aagctgattg ccaggggcag
     7861 gcccttggtg taagtgttta caaagcggtt aagctgggat gggtgcatac gtggggatat
     7921 gagatgcatc ttggactgta tttttaggtt ggctatgttc ccagccatat ccctccgggg
     7981 attcatgttg tgcagaacca ccagcacagt gtatccggtg cacttgggaa atttgtcatg
     8041 tagcttagaa ggaaatgcgt ggaagaactt ggagacgccc ttgtgacctc caagattttc
     8101 catgcattcg tccataatga tggcaatggg cccacgggcg gcggcctggg cgaagatatt
     8161 tctgggatca ctaacgtcat agttgtgttc caggatgaga tcgtcatagg ccatttttac
     8221 aaagcgcggg cggagggtgc cagactgcgg tataatggtt ccatccggcc caggggcgta
     8281 gttaccctca cagatttgca tttcccacgc tttgagttca gatgggggga tcatgtctac
     8341 ctgcggggcg atgaagaaaa cggtttccgg ggtaggggag atcagctggg aagaaagcag
     8401 gttcctgagc agctgcgact taccgcagcc ggtgggcccg taaatcacac ctattaccgg
     8461 gtgcaactgg tagttaagag agctgcagct gccgtcatcc ctgagcaggg gggccacttc
     8521 gttaagcatg tccctgactc gcatgttttc cctgaccaaa tccgccagaa ggcgctcgcc
     8581 gcccagcgat agcagttctt gcaaggaagc aaagtttttc aacggtttga gaccgtccgc
     8641 cgtaggcatg cttttgagcg tttgaccaag cagttccagg cggtcccaca gctcggtcac
     8701 ctgctctacg gcatctcgat ccagcatatc tcctcgtttc gcgggttggg gcggctttcg
     8761 ctgtacggca gtagtcggtg ctcgtccaga cgggccaggg tcatgtcttt ccacgggcgc
     8821 agggtcctcg tcagcgtagt ctgggtcacg gtgaaggggt gcgctccggg ctgcgcgctg
     8881 gccagggtgc gcttgaggct ggtcctgctg gtgctgaagc gctgccggtc ttcgccctgc
     8941 gcgtcggcca ggtagcattt gaccatggtg tcatagtcca gcccctccgc ggcgtggccc
     9001 ttggcgcgca gcttgccctt ggaggaggcg ccgcacgagg ggcagtgcag acttttgagg
     9061 gcgtagagct tgggcgcgag aaataccgat tccggggagt aggcatccgc gccgcaggcc
     9121 ccgcagacgg tctcgcattc cacgagccag gtgagctctg gccgttcggg gtcaaaaacc
     9181 aggtttcccc catgcttttt gatgcgtttc ttacctctgg tttccatgag ccggtgtcca
     9241 cgctcggtga cgaaaaggct gtccgtgtcc ccgtatacag acttgagagg cctgtcctcg
     9301 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     9361 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     9421 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     9481 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     9541 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     9601 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     9661 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     9721 cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10201 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10621 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10861 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10981 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11161 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11221 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11401 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11461 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11521 tccccgaaaa gtgccac
//
