LOCUS       sequence-192597-       11538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 17 of murine
            Fgfr3 and intron 6 of murine Tacc3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Cook PJ, Thomas R, Kannan R, de Leon ES, Drilon A, Rosenblum MK, 
            Scaltriti M, Benezra R, Ventura A
  TITLE     Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent 
            glioma driver and therapeutic target.
  JOURNAL   Nat Commun. 2017 Jul 11;8:15987. doi: 10.1038/ncomms15987.
  PUBMED    28695888
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-07-11"; volume: "8:15987. doi"; pages: " 
            10.1038/ncomms15987"
COMMENT     Sequence Label: Adeno FT
FEATURES             Location/Qualifiers
     source          1..11538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   16..118
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     206..356
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        406..654
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        676..751
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        842..1090
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1112..1187
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1277..1562
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1564..1841
                     /label=chicken beta-actin promoter
     intron          1842..2070
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2082..2091
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2091..2156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2163..2183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2208..6308
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2208..6308
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6309..6356
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6379..6395)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6379..6395)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6415..6549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6439..6458)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6493..6512
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9791..10379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9871..9890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10550..11341,11342..11410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11173..11192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11411..11515)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
       61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
      121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
      181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
      241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
      301 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatattt
      361 gtctagggag atccggtacc gtttaaactc gagaatggcg catgtgaggg cctatttccc
      421 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
      481 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      541 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      601 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggttt
      661 tatcggggta tgttggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      721 tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag aaatagcaag
      781 ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa atggctctag
      841 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      901 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      961 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     1021 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     1081 acgaaacacc ggttcaggct atgcccccta agttttagag ctagaaatag caagttaaaa
     1141 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag
     1201 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc
     1261 agacaaatgg ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1321 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
     1381 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1441 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
     1501 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1561 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     1621 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     1681 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
     1741 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     1801 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgacgctgc
     1861 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     1921 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
     1981 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
     2041 gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg gactataagg
     2101 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
     2161 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
     2221 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2281 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2341 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2401 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2461 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     2521 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     2581 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     2641 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     2701 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     2761 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     2821 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     2881 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     2941 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3001 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3061 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3121 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3181 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3241 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3301 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3361 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3421 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3481 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     3541 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     3601 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     3661 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     3721 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     3781 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     3841 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     3901 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     3961 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4021 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4081 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4141 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4201 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4261 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4321 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4381 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4441 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4501 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     4561 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     4621 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     4681 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     4741 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     4801 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     4861 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     4921 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     4981 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5041 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5101 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5161 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5221 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5281 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5341 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5401 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5461 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     5521 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     5581 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     5641 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     5701 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     5761 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     5821 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     5881 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     5941 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6001 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6061 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6121 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6181 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6241 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6301 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag
     6361 aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gggagatcca
     6421 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     6481 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6541 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcasgg ggaggtgtgg
     6601 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcccggc
     6661 tgcctcgcgc gtttcggtga tgacggtgaa aacctcttga cacatgcagc tcccggagac
     6721 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6781 gggtgttggc gggtgtcggg gcgcagccat gaggtcgact ctagtccccg cggtggcaga
     6841 tctggaaggt gctgaggtac gatgagaccc gcaccaggtg cagaccctgc gagtgtggcg
     6901 gtaaacatat taggaaccag cctgtgatgc tggatgtgac cgaggagctg aggcccgatc
     6961 acttggtgct ggcctgcacc cgcgctgagt ttggctctag cgatgaagat acagattgag
     7021 gtactgaaat gtgtgggcgt ggcttaaggg tgggaaagaa tatataaggt gggggtctta
     7081 tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagcaccaac tcgtttgatg
     7141 gaagcattgt gagctcatat ttgacaacgc gcatgccccc atgggccggg gtgcgtcaga
     7201 atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc cgcaaactct actaccttga
     7261 cctacgagac cgtgtctgga acgccgttgg agactgcagc ctccgccgcc gcttcagccg
     7321 ctgcagccac cgcccgcggg attgtgactg actttgcttt cctgagcccg cttgcaagca
     7381 gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac ggctcttttg gcacaattgg
     7441 attctttgac ccgggaactt aatgtcgttt ctcagcagct gttggatctg cgccagcagg
     7501 tttctgccct gaaggcttcc tcccctccca atgcggttta aaacataaat aaaaaaccag
     7561 actctgtttg gatttggatc aagcaagtgt cttgctgtct ttatttaggg gttttgcgcg
     7621 cgcggtaggc ccgggaccag cggtctcggt cgttgagggt cctgtgtatt ttttccagga
     7681 cgtggtaaag gtgactctgg atgttcagat acatgggcat aagcccgtct ctggggtgga
     7741 ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt gtagatgatc cagtcgtagc
     7801 aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag caagctgatt gccaggggca
     7861 ggcccttggt gtaagtgttt acaaagcggt taagctggga tgggtgcata cgtggggata
     7921 tgagatgcat cttggactgt atttttaggt tggctatgtt cccagccata tccctccggg
     7981 gattcatgtt gtgcagaacc accagcacag tgtatccggt gcacttggga aatttgtcat
     8041 gtagcttaga aggaaatgcg tggaagaact tggagacgcc cttgtgacct ccaagatttt
     8101 ccatgcattc gtccataatg atggcaatgg gcccacgggc ggcggcctgg gcgaagatat
     8161 ttctgggatc actaacgtca tagttgtgtt ccaggatgag atcgtcatag gccattttta
     8221 caaagcgcgg gcggagggtg ccagactgcg gtataatggt tccatccggc ccaggggcgt
     8281 agttaccctc acagatttgc atttcccacg ctttgagttc agatgggggg atcatgtcta
     8341 cctgcggggc gatgaagaaa acggtttccg gggtagggga gatcagctgg gaagaaagca
     8401 ggttcctgag cagctgcgac ttaccgcagc cggtgggccc gtaaatcaca cctattaccg
     8461 ggtgcaactg gtagttaaga gagctgcagc tgccgtcatc cctgagcagg ggggccactt
     8521 cgttaagcat gtccctgact cgcatgtttt ccctgaccaa atccgccaga aggcgctcgc
     8581 cgcccagcga tagcagttct tgcaaggaag caaagttttt caacggtttg agaccgtccg
     8641 ccgtaggcat gcttttgagc gtttgaccaa gcagttccag gcggtcccac agctcggtca
     8701 cctgctctac ggcatctcga tccagcatat ctcctcgttt cgcgggttgg ggcggctttc
     8761 gctgtacggc agtagtcggt gctcgtccag acgggccagg gtcatgtctt tccacgggcg
     8821 cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg tgcgctccgg gctgcgcgct
     8881 ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag cgctgccggt cttcgccctg
     8941 cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc agcccctccg cggcgtggcc
     9001 cttggcgcgc agcttgccct tggaggaggc gccgcacgag gggcagtgca gacttttgag
     9061 ggcgtagagc ttgggcgcga gaaataccga ttccggggag taggcatccg cgccgcaggc
     9121 cccgcagacg gtctcgcatt ccacgagcca ggtgagctct ggccgttcgg ggtcaaaaac
     9181 caggtttccc ccatgctttt tgatgcgttt cttacctctg gtttccatga gccggtgtcc
     9241 acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca gacttgagag gcctgtcctc
     9301 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     9361 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     9421 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     9481 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     9541 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     9601 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     9661 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     9721 acgaccatca gggacagctt caaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10201 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    10261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10561 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10621 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10681 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10741 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10801 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10861 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10921 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10981 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11041 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11101 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11161 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11221 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11281 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11341 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11401 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11521 ttccccgaaa agtgccac
//
