LOCUS       sequence-19262-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf185
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             512..553
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             563..580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(603..620)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    609..720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctcggatc cagtaccctt caccgaattc cagctgacca ccatgacgga cgaacaaatt
       61 tatgcattct gtgatgctaa caaagacgat atacgatgta aatgtattta tcctgataaa
      121 agcatagtac ggataggaat agatacaaga ttaccctatt attgttggta cgagccatgt
      181 aaacgaagcg atgcgttgtt accagcctct ttaaaaaaaa atataacaaa atgcaatgta
      241 tcggattgta ccatttcatt gggaaacgtt tccattacag atagtaaatt agatgtaaat
      301 aatgtttgtg attccaaacg agtagctacc gagaatatag ctgtccgcta tctgaatcag
      361 gaaattagat accctattat agatatcaaa tggcttccga ttggattact gcgttaggca
      421 attcccgggg atccgaataa agggtcaaga caattctgca gatatccagc acagtggcgg
      481 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
      541 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
      601 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      661 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      721 gcattgtctg agtaggtgtc attctattct ggg
//
