LOCUS       sequence-192629-        3022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deletes synpcc7942_1377 (spoT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     ppGpp Controls Global Gene Expression in Light and in Darkness in S.
            elongatus.
  JOURNAL   Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 
            10.1016/j.celrep.2017.11.067.
  PUBMED    29241543
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.11"; journalName: "Cell Rep"; date: 
            "2017-12-12- 12"; volume: "21"; issue: "11"; pages: "3155-3165"
COMMENT     Sequence Label: spoT-
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tggatggcgg cataactggc gctagacatc ggggctaatc gacagtgtca aaccaaaacg
       61 gttagccagc cctgaacagc gatcgcttcc agccagacag ggcagccgtt tccacttcta
      121 ggcaggtcaa cgtccagact gccgttgatt cgagcttaac gttcccttac caagcactgg
      181 tcagcaagac ttccagcact tgattgggtt gaagactcgt cgccggcaag ccactcccaa
      241 aggctgatgc agctccgaca gcagcccccc cgactaaccc cacgggtcct ccaaccacta
      301 gtcctgccgc tgcgcctaca cctgcattgt tggcaacacg accgccatca gccgtaggcg
      361 ggccggtgat gacctctgaa atggcataga tcggtactga gactccattg aggacaagcg
      421 cttgtgcaac aaaccgactg ccctgctgat tagtttcaaa gcggccgagc actggcgtcc
      481 cgatcggtag gatgacctgg cccctgctat ccgtgacatt ttggtcaaca gtcatgacct
      541 cttgaattgg tagagcgctt tgcaagctca gaccaggacc tgtgtagcgg agccgcacca
      601 caaaccgatc gctcaagggt ttcaaggggg ttgtgctgag tgacgagcca ccgccagccg
      661 gcacggtcgg cagtggaata ctggactggg cccaagcggt cgaagctgac agtgcggcta
      721 acacgccagc gatcgcgact gcagatttgc gcatcatttc catccatcgc ctttcgcaca
      781 gctagcttgc cattttccca ccaggaatca agacctaggt ttgccgtttg caccaagaat
      841 gactggattc agacccagaa tcttgcgatt cagtccacta gcattcctgt cacccccctg
      901 actcacagga aattaacggt taagatcggt gcacaactgc cgagcttccc aatgaactgc
      961 tgcagttgca ccagccaaaa ggggagcgat cgcacgttcg ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agtcactcag ctatccaact
     2041 gatgctgata aataccgcct acaggcactt attgcgctgc aggagttagc aattgagccg
     2101 cgatcgccgc tagtttttgg gccgcaccgg cctcaccgcg aaccgctctg agttccgctt
     2161 gcatggccga cagtttctct gggttctgga gatagtcgag agcgatcgcg gcaatatcct
     2221 cgggatagta ttcgccgatc aactcgggca cgatcgcccg cttcgcccaa atattcggcc
     2281 aagccagcag acccactttg cgcagcgcca aggtattaat caggctagcg atcgcattgc
     2341 ccaacagtgg caccttgacc agcaagcccg gcaagccgtc ccaggctttc atcgcatccc
     2401 gtttttgggt tggcagcagc accaacatcg gcaccgccag cgcgcctaat tctgctgtat
     2461 tcgcgccgat cgtggtcagg cagatctggc actgggccaa ctcgacatag gctggaaatt
     2521 ccgtgatcaa ccttacggtt agcccttttt cggtcgtcag cacccaacct gtgggttgct
     2581 cttctaaacg ggcggaacta ccccgaaaca ttgcaatgac gggattttga tccgcatccg
     2641 catagcgagc gatctgctgc ggttgcacgg tcggtgcgag tggcagaata aactccatct
     2701 ctggttgctg agccgcgatc gcttcagccg ccgccagcat gaagggcaag ccgatctgca
     2761 atttagctgc tttggatccg ggcagaagac ccacccgcga tcgtgtggat tcagatccag
     2821 cctgcgctga gacctccgcc attaaatcgc caaccaactg aaacttgtga cggtctttct
     2881 gcggtgcttt ctcaatcacc tggggcgtca ttaccgcaaa ggcatcagcc cagccagtcc
     2941 agcgggcttc ccattccgcg tagatcaagc agcggtagcc caagcgtcgg gcaatccaga
     3001 gggcaaaaaa ctgatcgccg cc
//
