LOCUS       sequence-19264-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf187
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             642..683
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             693..710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catggataag aaaagtttgt ataaatactt
       61 actactacgt tcaactggag atacgcacag agccaaatct cccactataa tgacaagggt
      121 aaccaataat gtgtatttgg gaaattataa aaatgctatg gatgcaccat catctgaagt
      181 taagttcaaa tatgttttaa atttgacgat ggataaatat acattaccta actctaatat
      241 taatattatt catataccgt tggtagatga tacaactacc gatattagta aatattttga
      301 cgacgtaacc gcctttttat ctaaatgtga tcaacgaaac gagcccgtgt tggttcattg
      361 tgctgcggga gtaaatagaa gcggggctat gattttggca tatctaatgt ctaaaaataa
      421 ggagtcattg cctatgctat attttttata cgtgtatcat tctatgaggg acttgagagg
      481 cgcatttgtg gaaaatccat cgtttaaaag acagatcata gaaaaatatg ttattgataa
      541 gaattaagca attcccgggg atccgaataa agggtcaaga caattctgca gatatccagc
      601 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
      661 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaa
//
