LOCUS       sequence-192641-        1743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942
            genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     ppGpp Controls Global Gene Expression in Light and in Darkness in S.
            elongatus.
  JOURNAL   Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 
            10.1016/j.celrep.2017.11.067.
  PUBMED    29241543
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.11"; journalName: "Cell Rep"; date: 
            "2017-12-12- 12"; volume: "21"; issue: "11"; pages: "3155-3165"
COMMENT     Sequence Label: relA+_NS1
FEATURES             Location/Qualifiers
     source          1..1743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(205..210,211..227,228..234)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    242..258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1717..1740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctgctccag aagctcgaaa attcgagctc gactgcacgg tgcaccaatg cttctggcgt
       61 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
      121 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
      181 tggcaaatat tctgaaatga gctgttgaca attaatcatc cggctcgtat aatgtgtgga
      241 attgtgagcg gataacaatt tcatacgctc acaattggta ccggtgatac cagcatcgtc
      301 ttgatgccct tggcagcacc ctgctaagga ggcaacaaga tggttgcggt aagaagtgca
      361 catatcaata aggctggtga atttgatccg gaaaaatgga tcgcaagtct gggtattacc
      421 agccagaagt cgtgtgagtg cttagccgaa acctgggcgt attgtctgca acagacgcag
      481 gggcatccgg atgccagtct gttattgtgg cgtggtgttg agatggtgga gatcctctcg
      541 acattaagta tggacattga cacgctgcgg gcggcgctgc ttttccctct ggcggatgcc
      601 aacgtagtca gcgaagatgt gctgcgtgag agcgtcggta agtcggtcgt taaccttatt
      661 cacggcgtgc gtgatatggc ggcgatccgc cagctgaaag cgacgcacac tgattctgtt
      721 tcctccgaac aggtcgataa cgttcgccgg atgttattgg cgatggtcga tgattttcgc
      781 tgcgtagtca tcaaactggc ggagcgtatt gctcatctgc gcgaagtaaa agatgcgccg
      841 gaagatgaac gtgtactggc ggcaaaagag tgtaccaaca tctacgcacc gctggctaac
      901 cgtctcggaa tcggacaact gaaatgggaa ctggaagatt actgcttccg ttacctccat
      961 ccaaccgaat acaaacgaat tgccaaactg ctgcatgaac ggcgtctcga ccgcgaacac
     1021 tacatcgaag agttcgttgg tcatctgcgc gctgagatga aagctgaagg cgttaaagcg
     1081 gaagtgtatg gtcgtccgaa acacatctac agcatctggc gtaaaatgca gaaaaagaac
     1141 ctcgcctttg atgagctgtt tgatgtgcgt gcggtacgta ttgtcgccga gcgtttacag
     1201 gattgctatg ccgcactggg gatagtgcac actcactatc gccacctgcc ggatgagttt
     1261 gacgattacg tcgctaaccc gaaaccaaac ggttatcagt ctattcatac cgtggttctg
     1321 gggccgggtg gaaaaaccgt tgagatccaa atccgcacca aacagatgca tgaagatgca
     1381 gagttgggtg ttgctgcgca ctggaaatat aaagagggcg cggctgctgg cggcgcacgt
     1441 tcgggacatg aagaccggat tgcctggctg cgtaaactga ttgcgtggca ggaagagatg
     1501 gctgattccg gcgaaatgct cgacgaagta cgtagtcagg tctttgacga ccgggtgtac
     1561 gtctttacgc cgaaaggtga tgtcgttgat ttgcctgcgg gatcaacgcc gctggacttc
     1621 gcttaccaca tccacagtga tgtcggacac cgctgcatcg gggcaaaaat tggcgggcgc
     1681 attgtgccgt tcacctacca gctgggcagc ggcagcgatt acaaagatga tgatgataaa
     1741 tag
//
