LOCUS       sequence-192651-        2934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit part-entry vector position 9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1699 HC_Kan_RFP-p9
FEATURES             Location/Qualifiers
     source          1..2934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..39
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(46..73)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(89..160)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(205..882)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             889..900
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    918..934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(942..948,949..966,967..972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    987..1008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1109..1130
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1214..1245
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1246..1348
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1349..2164
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1422..1441)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2185..2212
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2224..2811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2304..2323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2833..2862)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cgtctcgagc aagagaccct gcagcggccg ctactagtat ataaacgcag aaaggcccac
       61 ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac
      121 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtagcga
      181 tctacactag cactatcagc gttattaagc accggtggag tgacgacctt cagcacgttc
      241 gtactgttca acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt
      301 tttgtaagca cccggcagct gaaccggttt tttagccatg taggtggttt taacttcagc
      361 gtcgtagtga ccaccgtctt tcagtttcag acgcattttg atttcacctt tcagagcacc
      421 gtcttccggg tacatacgtt cggtggaagc ttcccaaccc atggtttttt tctgcataac
      481 cggaccgtcg gacgggaagt tggtaccacg cagtttaact ttgtagatga actcaccgtc
      541 ttgcagggag gagtcctggg taacggtaac aacaccaccg tcttcgaagt tcataacacg
      601 ttcccatttg aaaccttccg ggaaggacag tttcaggtag tccgggatgt cagccgggtg
      661 tttaacgtaa gctttggaac cgtactggaa ctgcggggac aggatgtccc aagcgaacgg
      721 cagcggacca cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc
      781 ttcaccttca ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt
      841 gaaacgcatg aactctttga taacgtcttc ggaggaagcc atctagtatt tctcctcttt
      901 ctctagtatg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      961 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1021 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1081 cgcgcgggga gaggcggttt gcgtattgct ctagaagcgg ccgcgaattc ggtctctacg
     1141 ccgagacgtg acgaattctc tagatatcgc tcaatactga ccatttaaat catacctgac
     1201 ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag
     1261 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
     1321 gagattttca ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc
     1381 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1441 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     1501 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1561 gctaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1621 tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga
     1681 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1741 gcattcgatt cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca
     1801 ggcgcaatca cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa
     1861 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga
     1921 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1981 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2041 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2101 tggtattgat aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt
     2161 ctgagggccc aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc
     2221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag
     2281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2701 tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2821 ttgattttct accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgcagtc
     2881 cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt cgtt
//
