LOCUS       sequence-192654-        4707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Useful for making infectious clones of RNA virus cDNAs; Has 35S 
            promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Lindbo JA
  TITLE     TRBO: a high-efficiency tobacco mosaic virus RNA-based 
            overexpression vector.
  JOURNAL   Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
  PUBMED    17720752
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007-12"; volume: "145(4)"; pages: "1232-40. Epub 
            2007 Aug 24"
COMMENT     Sequence Label: pJL 89
FEATURES             Location/Qualifiers
     source          1..4707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(156..1304)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(1603..2397)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2606..3241
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    3321..3345
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3425..3441
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3426..3442
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4161..4181
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        4170..4215
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    join(4685..4707,1..2)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 acctaagaga aaagagcgtt tattagaata atcggatatt taaagggcgt gaaaaggttt
       61 atccgttcgt ccatttgtat gtgcatgcca accacaggag atctcagtaa agcgctggct
      121 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
      181 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
      241 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
      301 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
      361 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
      421 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
      481 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
      541 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
      601 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
      661 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
      721 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
      781 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
      841 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
      901 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
      961 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
     1021 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
     1081 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
     1141 tcgatcttgg ccgtagcttg ctgggccatc gagccgacgg actggaaggt ttcgcggggc
     1201 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
     1261 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
     1321 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
     1381 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
     1441 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
     1501 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
     1561 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
     1621 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     1681 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     1741 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     1801 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     1861 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatgggg tgtcttcttc
     1921 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     1981 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     2041 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     2101 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     2161 aaggtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     2221 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     2281 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
     2341 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
     2401 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
     2461 acgaactcca attcactgtt ccttgcattc taaaacttta aataccagaa aacagctttt
     2521 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
     2581 caattatggg tgatgctgca actcgagcgg gccgggaggg ttcgagaagg gggggcaccc
     2641 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
     2701 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
     2761 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
     2821 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
     2881 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
     2941 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
     3001 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
     3061 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
     3121 ccacaacgcc ggcggccggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc
     3181 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt
     3241 ctctagtgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     3301 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     3361 caacttaata acacattgcg gacgttttta aagtactggg gtggttttgg taccgggccc
     3421 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcaggt caacatggtg
     3481 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
     3541 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     3601 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     3661 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     3721 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     3781 caagtggatt gatgtgataa catggtggag cacgacacac ttgtctactc caaaaatatc
     3841 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     3901 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     3961 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     4021 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     4081 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     4141 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     4201 tttcatttgg agaggcctcc ttttttccta ggatgcagcc cgggtcggca tggcatctcc
     4261 acctcctcgc ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag
     4321 ggagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     4381 tcctgttgcc ggtcttgcga ctagagtccg caaaaatcac cagtctctct ctacaaatct
     4441 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     4501 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     4561 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     4621 agcccggccg ggggatccac tagcagattg tcgtttcccg ccttcagttt aaactatcag
     4681 tgtttgacag gatatattgg cgggtaa
//
