LOCUS       sequence-192658-        3022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deletes glgA locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: glgA-
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cttggctgat gccttggttg agattggttt tgacctgatc cactagctga tcaacctcac
       61 gatcgctgcg gacattaccc acagcaacga cacgacccaa aaactgttgg gttgcctgtg
      121 gaaatgctgt caaaagtcgc tgcagccgcc gccgaatcaa gcgcagatcc cgttggatga
      181 tttgctggcg agtcagaggg cgaggcatag caacgagagt tcaagtctgg gatcaccata
      241 gcctgttgtt gggtacgcct cccatcagac ctatcaatgg tcaagattcg cgactgacaa
      301 ttagcatctc agcgccaatc accggactgc gagccaagag gcgatcgcgc tcatctacga
      361 tttcgaggcg gcggaagcct agttcccgca cctgctgaaa gacctcttgc gccagttgat
      421 cctgctgact agccgcaagg ctgccccagc cgtcactcaa gcgcagacgg aggcggctac
      481 cgacaaagtt ggcctccacg cccaagatca gcgcctcact atagcgatcg ctaatcgctg
      541 agacttccga ctggatcgcc tcaatcaggc gggcattggg atcaatttcc gttggccgcg
      601 agggacgtgg cggttccggt gctggttttg gtgcgggggg cggcgtaacg ggaagggctt
      661 tcggcggcgg caataaatca ggagcgggct gcggttcgac gggcgtcacc acaggttctg
      721 gcagccgcac ttgcagtttg ggccaagata gcggttgttg gctcagccag actgcgatcg
      781 cgatcgccaa gcctgcgccg actagaccca gccagtcgcg cccttgcaaa cgccgatcga
      841 gttcaggacg atcgctgccg atcgctgctt ctgcagcttg cagtgccacc agttgttgcc
      901 gactccagcg attgagccgc gaccaaacgg ccacctgact cgcgcgccat gacccttgca
      961 gccgctgcca aaaccccata agtgatcgcc tcctccgacc ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc aggcagacct cagggggcaa
     2041 cgccctccac tatacgcctg cgccttaccg ggtttggcta gcagagccgt ggcagcgcag
     2101 aaactcggtc atatcagcag ctgatagggg cttcgagaag taatagccct gccccatatc
     2161 gcagcctagc tgccgcaacc agtagagctg agactcttct tcaatgcctt cggcaacaac
     2221 ctccaagcca aagctatggg ccagcgcgat cgtggcttgg gcgacctggc ggttatcccc
     2281 agccaagttg atttgatgga tgaaggagcg atcgattttg agggaattaa ccggcagcct
     2341 ttgcaagtag ctcagcgaag aataccctgt tccaaagtca tcgatgctca gctggactcc
     2401 catcgatttc agctcatcga gcagcttgag caactgctgg tcaatatctt cagcaacact
     2461 ggactcagta atttctagca tgagattctg agcggatatt tccgttcttt gcagaatatc
     2521 tttgatccag tcctcaaagt cttcgtgaca aatctgctgg gccgagatat tgacgtgcaa
     2581 gatgaagtcg ccaggagtaa tcaagtgatc ctgttgccag aggtgaatct gcaggcaggc
     2641 cttttcaaga ataaaattac cgagactgaa aattagtttc gatcgctcag cgagggtcac
     2701 aaattccaag gggggaatgt tgcccagctc aggatgaaac cagcgggcta atgcttccgc
     2761 cccgacgaga gcgccggtct caagatcgac gatgggctgg tagaaaagat caatatctgc
     2821 ttgcttaatt gcctcccgta gggctgtttc tagagcaagg ttcttgaggg aattggcaga
     2881 gagtttagcc gagaagatct gaatgccacg actgagggga ttgcgtttgg cagcatacat
     2941 tgctgtatcc gcgctgcgga gcaactcttc agcagcatcg atctgggcag agacaaaact
     3001 gatgccgatg ctgacaccga tg
//
