LOCUS       sequence-192660-        3022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deletes glgC locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: glgC-
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 caattgccct aagacagttg tcgtctttcg aagtctagtt aacattaggg gcgattcttt
       61 gtttccactg agtggaagca aacggtatca aggttgcagg cagactcaag gtctagattg
      121 cttcacagct tgtgtggcta tatttattat cttcattatt gatggtagtt gtgggtggat
      181 ttaagatgga aaagtaacag ataaatgtcg tctttaaggg cgatctagat cgtatcgttt
      241 ttaattccta ggtcggcatt tattaatcaa cctcgataca atattttttt gtaaaaactt
      301 ctagataaat gactcaagtc tcattgaaag tctggggtgt tgcctcccca gtcaattcaa
      361 gattaccaag gcctcgcatc gcctcttcta ttttgtttga aggggaccta acgtgttgcg
      421 ccaagctagt tctcgacaga gcatctcaag agcgcgttgc tcgcgggggg caaacagttg
      481 gagatcagcc agctcttgca agacttgttg ggtgaggtgg ctggcaaagc taccggcata
      541 gcgcagtaag agactgtagt agcgaaattg cggttggccg ttgcaatcgc ggtgaaaggc
      601 agcaatttct gcttcgctga ggcagtagac aggggtattg accggcacga tcgcgcgagg
      661 aatgccctgt tcgccgtagc gatcgccgcc ctctgtttcc gctaagctgc cgatcaaaac
      721 cgtggagagc agcgtgcggg tttcattgat taaatcaggc gtgaatagtg ggtcgggccc
      781 acttgaaaga cgcggcccgt tgttcagaaa gaggggatta aacaactgcg agttgtagac
      841 cactccgatc gcccaatggc gatcgccttc caaacgaaca aagctgccaa atccatagct
      901 ctcggcggct ggtggattgg agacatccat gtcgtcatcc acttggacaa cgtagtcaca
      961 gtgcgagttg gatttgacaa ctttgccgag gcgcatggtg ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaatcaatc tcccccaagt
     2041 caagcggcgc tgagacccag tgtctgccgg tgagtcagtc ttggcaagca aactgtgcct
     2101 ttgcgatttc ttaccctacg cagctccggg atcgatcgga ggtaaccaag gctacggaca
     2161 atggcgcggg caccagctgt tggtaaactg aggagcgatc gccgcttcag tccaaaggct
     2221 atgacgcaaa aatccgttgt tattgctccg tccattctgt cagcggattt cagccgcttg
     2281 ggcgacgatg tccgcgctgt tgaccaggct ggcgctgact ggattcacgt cgatgtgatg
     2341 gatggtcgct tcgtccctaa catcaccatt ggaccgctga tcgttgaagc gctgcgcccg
     2401 gtgacccaaa agccgttgga cgtccacttg atgatcgtcg agccggaaaa atatgtgccg
     2461 gatttcgcga aagcaggggc tgacatcatc tcggtccaag cagaagcttg cccccacctg
     2521 caccgcaact tggctcagat caaagacctc ggcaagcaag caggcgtcgt cctcaacccc
     2581 tctaccccag tcgaaaccct ggaatacgtg ctggagttgt gcgacctgat tttgatcatg
     2641 agcgtcaacc ctggcttcgg tggtcagagt ttcatcccag ctgtcctgcc gaaaatccgt
     2701 aagctgcgcg ccatgtgcga tgagcgtggc cttgatcctt ggattgaagt cgatggcggc
     2761 ttgaaagcca ataacacttg gcaggtgctg gaagccggtg ctaacgcaat tgtggcgggc
     2821 tcggcagtct tcaacgcgcc ggactatgct gaagcgatcg cggcgattcg caacagcaag
     2881 cgtcctgaac ttgtcactgc ttaggcttct cgctcaacgc tcagtggagc aatctgaatc
     2941 ttgcagccct tcagtggatc agtctgctga ggggttttgc tttaggatgg gcgatcgcga
     3001 gtagggacac ggatcgctgg ta
//
