LOCUS       sequence-192662-        3022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deletes gnd locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: gnd-
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gtctgtcgat cccagcagcg atagagatgg aggagttgat cgaaatagtg acgagctaat
       61 tccagctgag ggaaagactt caccgaaagc tgatcgctcc ggcaatccaa aacacgaact
      121 tgatgatctt caatattggc aactgcagga ctgagaatga cgatccagcg accgatatgt
      181 tgtcgttgcg tttccataag aaagacagat gattgtgcga ttaaagtagc gacaacaagg
      241 ctaacaaatc gttacctagg gcacgatttt gctctgaacc agagtcagtc agcacaacgc
      301 taaacttttg tagagcttta tcctcggctc ctaaacggtt gcgatcgcca tgttgattca
      361 gcctgctgca acgacgtcta ccccagaggt ggctctcgca atcatcaccc aaggtcaaca
      421 gtggttattg cagctgcgtg atgacttaga ttggattctc tatccgggga agtggggctt
      481 atttggcggc catttagaac cggatgaaac tccccttgcc gccctctatc gcgaactcca
      541 agaagagatt ggctactacc cctccgccgc aaccccactc tggacggaaa cagaagaaag
      601 ggtcactcgc tacctcttcc atgtgccttt gacctgtgaa ctatcgactt tgcggctcgg
      661 cgaagggcag gatttctgcc tcgtcgatgc cgccgcgatc gcccaaggtc gagttttctc
      721 gccccgttta cagcaggaac gagcgatcgg tgcgccccac cagcagattt tgctgcgctt
      781 ccaaggggag tattcgcaca agcttgcccc cgtcatcagg gcctgaccgg tgcgatcgcc
      841 gtcaagaatc cacccccagg cattcaattt ttctctgatc tgtaaataaa tccgcgatcg
      901 ccgccgtgat ccccgcaaag aaagtctaaa gaggttgcca gagaacccca aattggcagg
      961 ctaacctgag gtcattcgtt gtcctagcag gaattttcct ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agggcgacaa actccggtcg
     2041 ctgcgctggt cagacggacc aactcctttg gcagaatcag gactggcaag gcgatcggag
     2101 agcgtgatgg atgtgttggt ggtgcgcggc attcgcgcct acggctacac ggggttcttt
     2161 gatgccgagc aagaactggg ccagtggttt gaggtggact tgcgcgtctg ggtcgatctt
     2221 cagcctgctg gggacagcga tcagctcggc gacaccctcg actacagcca agcggtacag
     2281 gtcgtgcaaa cggggattcg ccagagtcgc tttcgcacga tcgagcgatt ggcggctgat
     2341 ctctgcgatc gcctgtttca gcagtttcca gccctaccgg aattggaact gacgttgcgg
     2401 aaactggccc ctcccattcc tgactttagt ggtgatgtca gcttggttct gcggcgatcg
     2461 cggaagttaa cgggtgcgga ttgatgctag caaggggtcg aagatgctcg ttctggagag
     2521 ctgacgagac tgttttatag acttcaatca agagataaaa gttgactcgc gatcgttaga
     2581 tttggggata taatcctgag ttctaatagc tctttttagg gttccaaact gtaagtttga
     2641 ggattcactt tggaacttaa gtcaatccga attgaattcg ggttggctct gattatcgtg
     2701 atcaattgag acctagccgc gatcgcaaat ccatagaacc cgtctattcc tatgacactc
     2761 gatttgcttg tagtcaactc taaaaattcg tgctagcgtc tgatcgcaat cctcaatcct
     2821 catgcagctt cgcaagcgag atggattgtt gcttgtcttg accgaagcaa aggcttgtcg
     2881 tagttccagc tgtcctcatt ctctaggtcg ttctgctatg agcctcgctg cagtcttggc
     2941 gatcgcaatt tttgtggcgg tgattctggc tgcctccttt gaactgaccg atctcacggt
     3001 agtcgccctg gtcggagcga tg
//
