LOCUS       sequence-192663-        3022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deletes zwf_fbp_opcA locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: zwf-fbp-opcA-
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ctcgcgaatt gacctgggtc actggcgtcg tcatggcagt gatcaccgtg accttcgggg
       61 tgaccggcta ctcgctgcct tgggaccaag tcggctactg ggccgtcaaa attgtctcgg
      121 gtgttcccga agcgattccg gtggttgggt ctgccatggt tgaactactg cggggcggcc
      181 aaagcgtcgg tcaggcaact ctgacccgct tctacagcct ccacaccttt gtcttgcctt
      241 ggctgattgc agtgtttatg ctgcttcact tcctgatgat ccggaagcaa ggcatctctg
      301 gtcccctcta agggcaaaca agcttgggtt tggcgctggt taaaacggtc agcgcctttc
      361 tcctctctac tgactcgaac gagaccttcc caaggagagc acgcatcatg tccatcttga
      421 agaagccgga tcttagcgat ccgattctgc gccaaaagct ggccaaaggt atgggccata
      481 actactatgg cgagcccgct tggcccaacg atttgctcta catgttccca gtggtgattc
      541 tgggaacgat cgcctgcctg accggcttgg cagttctgga tccagctttg gtgggtgaac
      601 cggctgatcc cttcgcgacc ccgctggaaa ttctgccgga gtggtacctc taccccgtct
      661 tccagatcct gcggatcgtc ccgaataagc tgctgggcat cgtcctgcag tccatgattc
      721 ctttgggctt gattgcaatt ccgttcatcg aaagcgtcaa caagttccaa aatcctttcc
      781 gtcgcccgat cgcaacggct gtcttcctgt ttggtacggt ctttaccctc tacctcggaa
      841 ttggtgcagc gttaccgatc gataagtcgc tgaccctcgg cttgttctaa gcaccgagag
      901 ttaatgctca ttcactgaca gtaaaagggg gtctgcaagc agaccccttt tttgtggtgc
      961 tgcaactcag gcagttaaca ctggcaaagc ggcttgcaac ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc aggaagacat ccccgcgaag
     2041 gctgcgcgaa tttccccttc agcgtagaaa gatccccgag aatcgacagc cttcttcaga
     2101 taaactcaag ggccgtgatc gataatgctg cggagctttg gggtttgagg actagctcag
     2161 ccagagaaat ccgatcgtcc taggctcgac agaatgcgat cgcaccccag gcccagactg
     2221 ctttgaaacg gctcgcaggc acagccaaag gatgcgatcg cacagagccc caatcgggat
     2281 gactacatca gggctgggga gagctgctgg ttcagccaga cttctgcttg acgcgaggtt
     2341 tctaattcag tccgtccact gacaaccgcc agcaggcgat cgccttgaaa aatatggcca
     2401 aggtagcttg gctgttgacc tttttgatga tgaatgcgaa cacgaacgga ggtcatatac
     2461 ccctcctgta caagtgaggt gaaggcttgg gggaaagcta agcaagcatg agcatttagt
     2521 tcagagatta gcgatcgctc tgtcagggat ctcaaatctc accaactgat ttaacgaatg
     2581 gcgggaacat tcagcaaaac aaaggctggg ccaatctttt cgcaggctaa cgttgacgct
     2641 ctggccctcg gcgatcgccc ctctcaattg gggctggcag gcaattgagc agcgctgaca
     2701 gcggagtccc cacagcctgg aatcgtgccc tcatttaata gctgcaaccg ctgagccaaa
     2761 cgcgcccatt ggaaactacg cgggtccatg cgtgacccac ttctatccac agctgcatgg
     2821 gtggtaatgc gatcgccggg gattgtataa gttgtcatcc actgctgggc aagcagggcc
     2881 aacgagtcgt actgtcgagc cgtgtaacca ctgtgtcgcc ggcgattatc acggccatcg
     2941 gctggcgatt caaggctgac atggagcgag aaattgttga ccgagcccgg cagtttggga
     3001 ttaaatcgca cggcttcgcc cc
//
