LOCUS       sequence-192666-        2949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  deletes glgP_gap1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: glgP-gap1-
FEATURES             Location/Qualifiers
     source          1..2949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1034..1849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1757..1776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1841..1860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 caaaccaatc agcagttcgc tgatgcccaa ggtgcgagca accgcgacag aattatcaat
       61 caggagcttt gccccgagga tcaggagccc cagaccagct gcgattagca agagttgctg
      121 ccataggggt gggcgatcgc ccaatccata ttcctcggca tattccgcct gaacctctgg
      181 gtcaggttct tctcccccct ggcgaacttg gaaaacggtg tagagaacgc ccaacaccac
      241 caagacaatg ccatcgaggc ggctgatccg accatctagg gccatcccat agaccaatcc
      301 tgccgtggca atcatgatcg gcacatcgag ccgaaccaac tgttgcgcca cctgtagggg
      361 tgtgattaag gccgagatcc cgagaataaa caacacattg aagatgttgc tgcccaccac
      421 attcccgagc gaaatatcgt tggagccctc cagaaccgcc tgaatactga ctgcgagctc
      481 tggagcgctc gtcccgtagg ccaccacagt gaggccaatg atgagtggtg ataaccccac
      541 gagtgccgcc agttttgagg ctccgcggac taaaaactca gctccggcaa cgaggataac
      601 taaaccgagc agcaagaaca gtaaattgat cagcattgag gcgatggcca acatcacccc
      661 ttcatcttac ggacgcgtct gcgatcgccc ataaaaaaac cccgcgtggg cagggtttaa
      721 cggtattgct ctctgactac tgactgggaa tggaaatcta cagcaggcca atttgagaca
      781 ggatgccttg gccggtcagc aattcggtgg ccaaaccaac cacgaacccg atcatagcca
      841 gacggccatt ccaggtttct gcgaatgcgg tgaaaccaaa cttggtattt tgctcagaca
      901 tgacagggac ctccctacgg ggtggtttgg tagctgttaa gcaatgtaac gcaaaaactc
      961 aaaatccgca acatttcttc aaaaaatctt ggttcagttc ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agggtagccg ggagagggga
     2041 attgccctga ctatagacag gggatcacgg cggtcgatcg gccttaagag gaatgaaaga
     2101 aagccgtagt gctttgcaat gctcttctca gatcaggttt gggatcccat gcagatcaaa
     2161 ttcggctcag ccgttggctg ggcaggctgg tagaaccggc aagacgtgat tgaatgaagg
     2221 cgatcctttt atccggcgga cccatgcctg cgatcgcctc gcgctttctt ttggtactgc
     2281 tttgcttttt accgctagca gcctgtcgta gcttgggtgg caatgagact gagagcaata
     2341 gtcgcctcaa ccaagtgcag gcgcgcggta agttgctctg cggagttgaa ggccgactgc
     2401 ctggctttag cttccttgat tcccagggca attacagtgg cttggatgtc gacatttgta
     2461 aggcgatcgc cgcagcactg ttcaatgatc ccaaggcgat cgaataccgc agtctcgatt
     2521 ctgttgaacg gtttccagcc ttagcaagtg gtgaagtcga tttgctctcg cgcaacacca
     2581 cttggaccct gagtcgcgat gccaagggcg gcaacaacct tgagtttgcg ccaaccactt
     2641 tctatgacgg ccaagggttg atggtgcggc gcaatagtgg catccaatcg ctgcaggatt
     2701 ttcagggcaa gtcaatctgc gtggagacgg gcaccacatc ggaactcaat ctggcggaca
     2761 cgatgcgcga gctgggtgtc caatatcagg agattaaatt tccgaactcc gatgccaact
     2821 acgctgccta tgcccaaggc cgttgtgagg gggtaacctc cgatcgctct cagctggccg
     2881 cccgtcggac gaccttgtcc gatgccgatc agcaccaatt gctggatgca gtgatctcca
     2941 aagagccgc
//
