LOCUS       sequence-192667-        5275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces native promoter of zwf_fbp_opcA with Ptrc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::zwf_fbp_opcA
FEATURES             Location/Qualifiers
     source          1..5275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1734..2525
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2701..2723
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2801..2878
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2879..3961
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2898..2917)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(4193..4198,4199..4215,4216..4222)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4230..4246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4235..4257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ggcgaagccg tgcgatttaa tcccaaactg ccgggctcgg tcaacaattt ctcgctccat
       61 gtcagccttg aatcgccagc cgatggccgt gataatcgcc ggcgacacag tggttacacg
      121 gctcgacagt acgactcgtt ggccctgctt gcccagcagt ggatgacaac ttatacaatc
      181 cccggcgatc gcattaccac ccatgcagct gtggatagaa gtgggtcacg catggacccg
      241 cgtagtttcc aatgggcgcg tttggctcag cggttgcagc tattaaatga gggcacgatt
      301 ccaggctgtg gggactccgc tgtcagcgct gctcaattgc ctgccagccc caattgagag
      361 gggcgatcgc cgagggccag agcgtcaacg ttagcctgcg aaaagattgg cccagccttt
      421 gttttgctga atgttcccgc cattcgttaa atcagttggt gagatttgag atccctgaca
      481 gagcgatcgc taatctctga actaaatgct catgcttgct tagctttccc ccaagccttc
      541 acctcacttg tacaggaggg gtatatgacc tccgttcgtg ttcgcattca tcatcaaaaa
      601 ggtcaacagc caagctacct tggccatatt tttcaaggcg atcgcctgct ggcggttgtc
      661 agtggacgga ctgaattaga aacctcgcgt caagcagaag tctggctgaa ccagcagctc
      721 tccccagccc tgatgtagtc atcccgattg gggctctgtg cgatcgcatc ctttggctgt
      781 gcctgcgagc cgtttcaaag cagtctgggc ctggggtgcg atcgcattct gtcgagccta
      841 ggacgatcgg atttctctgg ctgagctagt cctcaaaccc caaagctccg cagcattatc
      901 gatcacggcc cttgagttta tctgaagaag gctgtcgatt ctcggggatc tttctacgct
      961 gaaggggaaa ttcgcgcagc cttcgcgggg atgtcttccc ggatcaattc ccctgctcgc
     1021 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
     1081 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
     1141 cactgatccg catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca
     1201 gaaaaccgag gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg
     1261 gccgaggtct tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga
     1321 agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg
     1381 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac
     1441 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1501 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1561 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1621 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1681 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
     1741 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1801 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     1861 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     1921 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     1981 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2041 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2101 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2161 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2221 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2281 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2341 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2401 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2461 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2521 aataatgcta gctcgagccc gcgacatctt cctgctccag aagctcgaaa ttcgagctcg
     2581 gtacccgggg atctgggccg cgggtcatgg ctgcgccccg acacccgcca acacccgctg
     2641 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2701 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga
     2761 tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt gacaccatcg aatggtgcaa
     2821 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt
     2881 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     2941 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     3001 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     3061 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     3121 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     3181 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     3241 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     3301 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     3361 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     3421 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
     3481 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
     3541 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
     3601 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
     3661 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
     3721 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     3781 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
     3841 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
     3901 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3961 agcgcaacgc aattaatgta agttagcgcg aattgatctg gtttgacagc ttatcatcga
     4021 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
     4081 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
     4141 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
     4201 taatcatccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     4261 cagaccatgg aattcatggc tcaatccacc acttccgaga cccacaccag agatctcgat
     4321 cgggattgca ccaccctgtc gcgccatgtt ctcgaacagt tgcaaagctt tagccccgaa
     4381 gctcaagacc ttgcggcgtt gatgcagcgc attggccttg ccgctaagct aatcgcccgt
     4441 cgcctcagcc acgcggggct ggtggatgat gccctcggtt tcacggggga aatcaacgtc
     4501 cagggcgaag ccgtcaaacg gatggacgtc tacgctaacc aagttttcat ctcagttttt
     4561 cggcagagtg gcttggtctg tcggctggct tccgaggaaa tggagaagcc ctactacatt
     4621 cctgaaaatt gcccgatcgg tcgctatacg ctgctctacg accctttaga tggctcggcc
     4681 aacgtcgatg tcgatctcaa cgtcggttcc atttttgcgg tgcggcgtca agagttctac
     4741 gacgagtccc atgaagccaa ggatcttctg cagccaggcg atcgccaaat tgctgcgggc
     4801 tatgtcctct atggggccag caccttgctg gtctacagca tgggtcaggg cgtccatgtt
     4861 tttgtactgg accccagctt aggtgagttt gtcctcgcgc agtccgacat ccagctcccg
     4921 aactcgggtc agatctacag cgtcaatgaa gggaactttt ggcagtggcc cgagggctat
     4981 cgccagtaca tccgggaaat gcatcgccgc gaaggctaca gcggccgcta tagcggagcg
     5041 ttggtagctg acttccatcg cattttgatg cagggtggtg tgtttctcta tcccgaaacc
     5101 gtcaagaatc ccactggcaa gctgcgactg ctctacgaag cagcaccgat ggcctttctg
     5161 gctgaacaag caggcggcaa ggccagtgac ggccagaagc ctatcctctt gcgccaaccc
     5221 caagccctgc acgagcggtg cccgctaatt atcggtagcg ccgcagatgt ggact
//
