LOCUS       sequence-19267-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf190
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             556..597
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             607..624
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(647..664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccgaatttca gctgaccacc atgtccatca atatcgatat aaaaaaaata actgatttac
       61 tcaacagtag tattttattc ccagatgatg tgcaagaact tcttcgagag aaatatatag
      121 tattagaaag aaaatcaaat ggtacaccta cagtagctca catctataag acaatggcta
      181 gatttgataa taagagtata tatagaatcg ccaagttttt atttatgaac aggccaggtg
      241 ttatcaaact tttattttta aaagacgtag aacctctgtt acccgacaaa agtattaata
      301 tatctattaa caatacagag tatccacagt tggaaggtcc tataggaaca aaaatcgctc
      361 tattggaatt atttaatgca tttagaactg ggagatcaga acccatacca tattattatt
      421 taccgcttag aaaagacata aacaacatag taactaagta agcaattccc ggggatccga
      481 ataaagggtc aagacaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag
      541 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
      601 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
      661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgg cttccttg
//
