LOCUS       sequence-192674-        3145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  drives expression of galP transporter from NS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::galP_NS1
FEATURES             Location/Qualifiers
     source          1..3145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     176..198
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        276..353
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             354..1436
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(373..392)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1668..1673,1674..1690,1691..1697)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1705..1721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1710..1732
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tgctagctcg agcccgcgac atcttcctgc tccagaagct cgaaattcga gctcggtacc
       61 cggggatctg ggccgcgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
      121 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      181 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat
      241 tcgcgcgcga aggcgaagcg gcatgcattt acgttgacac catcgaatgg tgcaaaacct
      301 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
      361 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
      421 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
      481 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
      541 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
      601 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
      661 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
      721 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
      781 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
      841 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
      901 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
      961 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
     1021 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
     1081 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
     1141 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
     1201 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     1261 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     1321 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1381 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     1441 aacgcaatta atgtaagtta gcgcgaattg atctggtttg acagcttatc atcgactgca
     1501 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
     1561 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
     1621 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
     1681 atccggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagac
     1741 catggaattc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     1801 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     1861 tggcgcactg ccgtttattg cagatgaatt ccagattact tcgcacacgc aagaatgggt
     1921 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt
     1981 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct
     2041 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcccgcgttc tactggggct
     2101 ggcggtgggt gtggcctctt ataccgcacc gctgtacctc tctgaaattg cgccggaaaa
     2161 aattcgtggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     2221 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     2281 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     2341 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     2401 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     2461 tggctgggcg ctgtttaaag agaacagcaa cttccgccgc gcggtgttcc ttggcgtact
     2521 gttgcaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     2581 cttcgaactg gcgggttata ccaacactac cgagcaaatg tgggggaccg tgattgtcgg
     2641 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     2701 accaacgcta acgctgggct tcctggcgat ggctgctggc atgggcgtac tcggtacaat
     2761 gatgcatatc ggtattcact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     2821 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     2881 tcagccgctg aaaggccgcg attttggcat cacctgctcc actgccacca actggattgc
     2941 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     3001 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     3061 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     3121 gcgcgaaata ggcgctcacg attaa
//
